Browsing by Author "Akbaba, Giray Bugra"

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Citation - WoS: 4
Citation - Scopus: 8
In Vitro Genotoxicity Evaluation of Tungsten (VI) Oxide Nanopowder Using Human Lymphocytes
(Allied Acad, 2016) Akbaba, Giray Bugra; Turkez, Hasan; Sonmez, Erdal; Akbaba, Ugur; Aydin, Elanur; Tatar, Abdulgani; Cerig, Salim
Tungsten (VI) oxide (or tungsten trioxide) (WO3, <100 nm particle size) nanoparticles (NPs) are used for many purposes including production of electrochromic windows, or smart windows, x-ray screen and gas sensors in everyday life. However, their carcinogenicity and genotoxicity have not been sufficiently evaluated. Therefore, the genotoxic potential of WO3 nanoparticle was examined in cultured human lymphocytes by the use of the micronucleus (MN) test and the comet (SCGE) assay. Freshly isolated human lymphocytes were exposed to WO3 nanoparticle at concentrations ranging from 0 to 500 mu M for 72 hours at 37 degrees C. Our results indicated that 400 and 500 mu M of WO3 nanoparticle treatment caused slight increases of the MN frequencies in cultured human lymphocytes. Likewise, WO3 nanoparticle (at concentrations above 200 mu M) led to increases of DNA damage (estimated with the comet assay) in human lymphocytes. The observed alterations in the MN and the comet assay parameters revealed that WO3 nanoparticles have genotoxic potential and could pose environmental and human health risk.
Citation - WoS: 20
Citation - Scopus: 22
Investigation of the Genotoxicity of Aluminum Oxide, -Tricalcium Phosphate, and Zinc Oxide Nanoparticles in Vitro
(Sage Publications inc, 2018) Akbaba, Giray Bugra; Turkez, Hasan
The aim of this study was to investigate the genotoxicity of aluminum oxide (Al2O3), -tricalcium phosphate (-TCP) (Ca-3(PO4)(2)), and zinc oxide (ZnO) nanoparticles (NPs) that were 4.175, 9.058, and 19.8 nm sized, respectively, on human peripheral blood lymphocytes using micronucleus (MN) and chromosome aberration (CA) techniques. Aluminum oxide and -TCP NPs did not show genotoxic effects on human peripheral blood cultures in vitro, even at the highest concentrations; therefore, these materials may be suitable for use as biocompatible materials. It was observed that, even at a very low dose (12.5 ppm), ZnO NPs had led to genotoxicity. In addition, at high concentrations (500 ppm and above), ZnO NPs caused mortality of lymphocytes. For these reasons, it was concluded that ZnO NPs are not appropriate for using as a biocompatible biomaterial.