Browsing by Author "Alayli, Azize"

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In Vitro Effects of CaO Nanoparticles Ontriticalecallus Exposed to Short and Long-Term Salt Stress
(Springer, 2021) Yazicilar, Busra; Boke, Fatma; Alayli, Azize; Nadaroglu, Hayrunisa; Gedikli, Semin; Bezirganoglu, Ismail
Key message Ca(2+)NPs enhanced tolerance ofTriticalecallus under salt stress by improving biochemical activity and confocal laser scanning analysis, conferring salt tolerance on callus cells. CaO NPs (Ca2+) are significant components that act as transducers in many adaptive and developmental processes in plants. In this study, effect of Ca(2+)NPs on the response and regulation of the protective system inTriticalecallus under short and long-salt treatments was investigated. The activation of Ca(2+)NPs was induced by salt stress in callus ofTriticalecultivars. MDA, H2O2, POD, and protein activities were determined in callus tissues. Concerning MDA, H2O2, protein activities, it was found that the Ca(2+)NPs treatment was significant, and it demonstrated a high correlation with the tolerance levels of cultivars.Tatlicakcultivar was detected for better MDA activities in the short time with 1.5 ppm Ca(2+)NPs concentration of 50 g and 100 g NaCl. Similarly, the same cultivar responded with better H(2)O(2)activity at 1.5 ppm Ca(2+)NPs 100 g NaCl in the short time. POD activities exhibited a decreasing trend in response to the increasing concentrations of Ca(2+)NPs. The best result was observed at 1.5 ppm Ca(2+)NPs 100 g NaCl in the short term. Based on the protein content, treatment of short-term cultured callus cells with 1.5 ppm Ca(2+)NPs inhibited stress response and it significantly promoted Ca(2+)NPs signals as compared to control callus. Confocal laser scanning analysis proved that the application of Ca(2+)NPs could alleviate the adverse effects of salt stress by the inhibition of stress severity in callus cells. This study demonstrated, under in vitro conditions, that the application of Ca(2+)NPs can significantly suppress the adverse effects of salt stress onTriticalecallus; it was also verified that the concentration of Ca(2+)NPs could be important parameter to be considered in adjusting the micronutrient content in the media for this plant.
Mitigation of Drought Stress Effects on Alfalfa (Medicago Sativa L.) Callus Through Cao Nanoparticles and Graphene Oxide in Tissue Culture Conditions
(Springer, 2024) Yazicilar, Buesra; Nadaroglu, Hayrunnisa; Alayli, Azize; Nadar, Muthukumar; Gedikli, Semin; Bezirganoglu, Ismail
Drought stress poses a significant threat to fertile soils worldwide, triggering profound physiological, biochemical, and molecular changes in plants that adversely impact agricultural productivity. This study explores the potential of nanotechnology, specifically Calcium Oxide Nanoparticles (CaO NPs) and Graphene Oxide (GO), to ameliorate the negative effects of drought stress on two distinct alfalfa ecotypes. Seeds from Erzurum and Konya regions were regenerated in the Murashige and Skoog (MS) medium, and ensuing callus formation was induced through 1 mg L-1 2,4-D and 1 mg L-1 kinetin MS medium. The callus samples underwent a one-month treatment with varying concentrations of mannitol (50 and 100 mM), CaO NPs, and GO (0.5 and 1.5 ppm). Results revealed a decrease in dry/wet weight with increasing mannitol concentration, contrasting with an increase in weight under CaO NPs and GO treatment. Proline, DNSA, MDA, and H2O2 exhibited proportional increases under drought stress, while CaO NPs and GO treatments mitigated these effects. Physiological and biochemical analyses identified optimal conditions for Erzurum as 50 mM mannitol/2 CaO NPs/0.5 ppm GO, and for Konya as 50 mM mannitol/0.5 ppm GO. Gene expression analysis indicated up-regulation of mtr-miR159 and mtr-miR393 with heightened drought stress, with down-regulation observed in CaO NPs and GO treatments. Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM) confirmed Ca2+ accumulation in alfalfa tissues. In conclusion, CaO NPs and GO treatments exhibited a significant reduction in the adverse effects of drought stress on alfalfa callus under tissue culture conditions. This research sheds light on the potential of nanotechnological interventions to alleviate the impact of environmental stressors on crop plants, opening avenues for sustainable agriculture in the face of changing climatic conditions. Further investigations are warranted to elucidate the underlying mechanisms and scalability of these findings for field applications.