Browsing by Author "Algur, Omer Faruk"

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The First Study on Bacterial Flora and Biological Control Agent of the Little Spruce Sawfly, Pristiphora Abietina (Christ.) (Hymenoptera: Tenthredinidae)
(Ars Docendi, 2017) Iskender, Nurcan Albayrak; Ortucu, Serkan; Algur, Omer Faruk; Aksu, Yasar; Saral, Aysegul
The aim of this study was to determine the bacterial flora of Pristiphora abietina and to find the performance of the members of this flora as a biocontrol agent for this pest. For this purpose, eleven bacteria were isolated from living, diseased and dead larvae. Morphological and biochemical properties, metabolic enzyme profiles by BIOLOG microtiter plate system and total cellular fatty acid profile by Microbial Identification Systems (MIS) of the bacterial isolates were determined. In addition, 16S rRNA gene sequence analysis was performed. The isolates were identified as Bacillus pumilus (Pa1), Lysinibacillus fusiformis (Pa2, Pa10), Stenotrophomonas maltophilia (Pa3), Acinetobacter johnsonii (Pa4, Pa9), Bacillus cereus (Pa5), Rhodococcus sp. (Pa6), Staphylococcus sciuri (Pa7), Ralstonia pickettii (Pa8), Neisseria perflava (Pa11). All these bacteria were tested against P. abietina larvae. The highest insecticidal activity was obtained from S. maltophilia and L. fusiformis (65.47%, 60.71%, respectively), (p < 0.05), whereas the lowest insecticidal activity (17.26%) was obtained from N. perflava within seven days. Our result indicates that L. fusiformis (Pa2, Pa10) show potential to be used as biological control agents of P. abietina.
L-Lactic Acid Production by Rhizopus Oryzae Mbg-10 Using Starch-Rich Waste Loquat Kernels as Substrate
(Wiley-V C H Verlag GmbH, 2013) Taskin, Mesut; Ortucu, Serkan; Unver, Yagmur; Arslan, Nazli Pinar; Algur, Omer Faruk; Saghafian, Amir
The objective of this work was to perform production of L-lactic acid from starch-rich waste loquat kernels by newly isolated Rhizopus oryzae MBG-10 fungus. Loquat kernel flour (LKF) was used as substrate (mainly as carbon source). The most favorable conditions for L-lactic acid production were LKF concentration of 80g/L, CaCO3 concentration of 20g/L, ammonium sulfate concentration of 3g/L and incubation time of 108h. Under these conditions, L-lactic acid and biomass concentrations were 45.4 and 8.2g/L, respectively, and -amylase activity was 81.6U/mL. No significant pH changes were observed in the medium thanks to the buffering capacity of LKF. L-lactic acid could be produced in a single-stage from starch-rich LKF without prior saccharification by the fungus with high amylolytic enzyme activity. This is the first report on use of waste loquat kernels as a lactic acid production substrate.
A Laboratory Assessment of Two Local Strains of the Beauveria Bassiana (Bals.) Vuill. Against the Tetranychus Urticae (Acari: Tetranychidae) and Their Potential as a Mycopesticide
(Hindawi Ltd, 2017) Ortucu, Serkan; Algur, Omer Faruk
This study was conducted to assess highly pathogenic Beauveria bassiana isolates to be used in biocontrol and to determine their potentials as mycopesticide. For this purpose, two B. bassiana isolates, which were locally isolated from T. urticae, were chosen. Firstly, three suspensions were investigated at the degree of humidity of 65 +/- 5% and 100% RH. Secondly, these strains were selected according to their tendency to mass production, tolerance to UV radiation, and capability of producing spore at the different temperatures. Finally, identification of the selected isolate was performed by using ITS rDNA analysis. Both tested fungal isolates were pathogenic to the T. urticae. Mycelial growths of isolate AT076 at 20 degrees C and 30 degrees C were found to be greater than isolate AT007. It was observed that isolate AT076 had more spore production with 1.61 x 107 spore/disc at 30 degrees C and 44.33% germination after UV radiation for 15 min. The numbers of spores per 5mm disk area for isolates AT076 and AT007 were found to be 1.2 x 106 and 1.0 x 106. These results show that isolate AT076 was more virulent and more UV-tolerant and had higher tendency to mass production compared to isolate AT007 against T. urticae. As a result of this study, isolate AT076 can be used in the biocontrol as mycopesticide.
The Preliminary Assessment and Isolation of Entomopathogenic Fungi to Be Used in Biological Control with Twospotted Spider Mite [Tetranychus Urticae (Acari, Tetranychidae)] from East Anatolia
(American Institute of Physics, 2017) Ortucu, Serkan; Algur, Omer Faruk
This study was conducted to isolation entomopathogenic fungi for possible use in biocontrol of two-spotted spider mite Tetranychus urticae Koch. and to determine their pathogenicity. For this purpose, plant leaves infected with T. urticae were collected from Erzurum, Kars and Ardahan. At laboratory, the internal and external mycoflora of T. urticae individuals on plant leaves were determined. As a result of isolation, twenty-five different fungi species belonging to the genera Acremonium, Alternaria, Aspergillus, Beauveria, Cladosporium, Gliocladium, Humicola, Penicillium, Trichoderma, Isaria, Ulocladium and Verticillium were obtained. Pathogenicity of this forty-five isolate belonging to twenty-five species were evaluated. As a test organism, T. urticae was used and suspensions (1x10(8) conidia ml(-1)) were prepared in Tween 80. 2ml suspension of a single dose was sprayed onto down side of bean leaf discs using hand sprayer. Mortality was recorded daily for 7 days. A total of twelve isolates belonging to three species were determined to be pathogen against T. urticae. According to scale used: AT020 Isaria farinosa and AT025 Cladosporium cladosporioides were determined as least pathogen, AT037 and AT101 Beauveria bassiana, and AT019 and AT026 C. cladosporioides, and AT035 and AT036 I. farinosa as moderate pathogen, AT007, AT021, AT034 and AT076 B. bassiana as highly pathogen. The other thirty-three isolates found that not pathogenic against T. urticae.