Browsing by Author "Aslan, A."

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An Antidote for Imazalil-Induced Genotoxicity in Vitro: The Lichen, Dermatocarpon Intestiniforme (Korber) Hasse
(Akademiai Kiado Zrt, 2012) Turkez, H.; Aydin, Elanur; Aslan, A.
Imazalil (IMA), a commonly used fungicide in both agricultural and clinical domains, is suspected to produce serious toxic effects in vertebrates. In recent years, a number of studies have suggested that lichens might be easily accessible sources of natural drugs that could be used as a possible food supplement. Extensive research is being performed to explore the importance of lichen species, which are known to contain a variety of pharmacological active compounds. In this context, the antigenotoxic effect of aqueous Dermatocarpon intestiniforme (Korber) Hasse. extract (DIE) was studied against the genotoxic damage induced by IMA on cultured human lymphocytes (n = 6) using chromosomal aberration (CA) and micronucleus (MN) as cytogenetic endpoints. Human peripheral lymphocytes were treated in vitro with varying concentrations of DIE (0, 25, 50 and 100 mu g/ml), tested in combination with IMA (336 mu g/ml). DIE alone were not genotoxic and when combined with IMA treatment, it reduced the frequency of CAs and the rate of MNs. A clear dose-dependent decrease in the genotoxic damage of IMA was observed, suggesting a genoprotective role of DIE. The results of the present study suggest that this plant extract per se does not have a genotoxic potential, but can alleviate the genotoxicity of IMA on cultured human lymphocytes. In conclusion our findings may have an important application for the protection of cultured human lymphocyte from the genetic damage and side effects induced by medical and agricultural chemicals hazardous for people.
The Effects of Lichens Extracts İn the Healthy Rats And the Medical Utility of These Extracts İn the Prevention of Diabetes-Associated Multiple Organ Failures
(Veteriner Fakultesi Dergisi vetdergi@kafkas.edu.tr, 2015) Çolak, S.; Geyikoǧlu, F.; Türkez, H.; Bakır, M.; Husseinigouzdaganii, M.; Can, S.; Aslan, A.
In the present study, we firstly assessed Cetraria islandica and Pseudevernia furfuracae to avoid detrimental effects on multiple tissues of rats. Diabetes mellitus (DM) with the subsequent generation of oxidative stress represents a major risk factor for organs. The second aim of this study is to investigate whether administration of both lichens could prevent type 1 diabetes (T1D)-induced organ dysfunctions. During two weeks, both control and diabetic rats were treated with aqueous lichen extracts. The metabolic changes were determined. On day 14, after animals were decapitated, required samples for biochemical and genetic analysis were collected. Oxidative damage of DNA was estimated by measuring the increase in 8-hydroxy-2’-deoxyguanosine formation. Biochemical parameters were used to observe and evaluate the functional changes in tissues. Experimental data showed that the increasing doses of lichens alone have not any detrimental effect on above parameters. Moreover, C. islandica decreased the diabetes-induced glucose and malondialdehyde (MDA) levels. Thus, it seemed that the antioxidant treatment has an important effect on the organ failure in ill rats. However, the protective effect of C. islandica was inadequate on diabetes-induced disorders and DNA damages. Lichens are a safe in the studied dose range but the power of C. islandica is limited because of intensive oxidative stress in essential organs of T1D rats. © 2015, Veteriner Fakultesi Dergisi. All rights reserved.
Evaluation of Antioxidant and Cytotoxic Effects of Olivetoric and Physodic Acid in Cultured Human Amnion Fibroblasts
(Sage Publications Ltd, 2017) Emsen, B.; Turkez, H.; Togar, B.; Aslan, A.
It is known that lichens are utilized for the treatment of many diseases including ulcer, diabetes, and cancer for many years. Secondary metabolites in the structure of the lichens provide various activity properties for them. In the present study, cytotoxic and oxidative effects of main constituents of Pseudevernia furfuracea (L.) Zopf (Parmeliaceae), olivetoric acid (OA), and physodic acid (PA) were investigated on cultured human amnion fibroblasts (HAFs). OA and PA were isolated from P. furfuracea using column chromatography and their structures were determined by proton nuclear magnetic resonance and carbon-13 nuclear magnetic resonance. HAFs were incubated during 48 h in the presence of OA and PA, at different concentrations from 6.25 mg/L to 200 mg/L. OA showed higher cytotoxicity than PA. In fact, median inhibitory concentration values of OA and PA were 571.27 and 3373.69 mg/L, respectively. The lower concentrations (<50 mg/L) of OA and PA did not cause oxidative stress and genotoxicity; furthermore, they supported anti-oxidative capacity of HAFs. Therefore, all these data suggested that both tested metabolites, especially PA might be developed as natural health medicine to protect human body against oxidative stress and genotoxicity. As far as we know, this is the first report on the cytotoxic and anti-oxidative activities of OA and PA on HAFs.