Browsing by Author "Celep, Nevra Aydemir"

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Effects of Dietary Thyme and Rosemary Essential Oils on Biochemical Parameters, Anti-Oxidant Metabolism, Small Intestinal Morphology and Myofiber Structure of Superficial Pectoral and Biceps Femoris Muscles in Broilers
(Urmia Univ, 2023) Gumus, Recep; Kara, Adem; Ozkanlar, Seckin; Imik, Halit; Celep, Nevra Aydemir
This study was aimed at determining the effects of dietary supplementation with thyme essential oil (TEO) and rosemary essential oil (REO) on blood parameters, the anti-oxidant metabolism in the liver, breast and drumstick muscle tissues, the morphology of the small intestine, and the myofibril structure of the superficial pectoral and biceps femoris muscles. For this purpose, 400 three-day-old male Ross 308 chicks were used. Five groups, each comprising 80 broilers, were established. The control group was fed on a basal diet alone and groups thyme -1, thyme-2, rosemary-1 and rosemary-2 received basal diets supplemented with 0.15 g kg-1 of TEO, 0.30 g kg-1 of TEO, 0.10 g kg-1 of REO and 0.20 g kg-1 of REO, respectively. The serum total cholesterol and low-density lipoprotein levels were decreased significantly in group thyme-1. Dietary TEO and REO significantly increased glutathione levels in all tissues. Drumstick catalase activity was significantly increased in groups thyme-1, thyme-2 and rosemary-2. Superoxide dismutase activity was significantly increased in the breast muscle of all groups that received dietary TEO and REO. Histomorphometrical analyses demonstrated that dietary supplementation with TEO and REO increased both crypt depth and villus height in the small intestine. In result, the tested doses of dietary TEO and REO were ascertained to improve the intestinal morphology and to increase the anti-oxidant metabolism mainly in the breast muscle, the drumstick muscle and liver. (c) 2023 Urmia University. All rights reserved.
Evaluating the Potential Adverse Effects of Favipiravir on Biochemical, Histopathological, and Spermatological Parameters in Male Rats’ Testicular Tissue
(Wiley, 2025) Omur, Ali Dogan; Ucak, Gamze; Kara, Adem; Erbas, Elif; Akarsu, Serkan Ali; Ozkanlar, Seckin; Celep, Nevra Aydemir
Favipiravir is a selective RNA polymerase inhibitor and a broad-spectrum antiviral drug. Favipiravir reduces cell proliferation by inhibiting RNA transcription, particularly in rapidly proliferating cells such as spermatogonia. The aim of this study was to investigate the effects and mechanism of action of favipiravir (T-705) on sperm quality and testicular tissue in rats. A total of 60 Sprague-Dawley rats, 30 in each group, were used in our study. Rats were randomly divided into two groups, control and experimental. Rats were killed on Day 14, Day 21, and Day 50 to observe short- and long-term effects. Oxidative stress, apoptosis, proliferation, aromatase activity, inflammation, histopathological changes, and epididymal sperm quality were examined in the testicular tissue of rats. Favipiravir administration decreased SOD activity and GSH levels and increased MDA levels and 8-OHdG levels in the testes of rats. It increased the levels of Caspase-3 and NF-& kgreen;B, which are apoptotic markers, and decreased the levels of NRF2, PI3K, and Bcl-2, which play a role in the regulation of apoptosis. Favipiravir led to disruption of the seminiferous tubules and disturbances in the structure of cells in the testis. In spermatological analysis, total motility value and epididymal spermatozoa density decreased. On Day 50, the favipiravir groups had higher rates of abnormal spermatozoa, DNA damage, and acrosome damage. In conclusion, favipiravir administration induced oxidative stress by increasing MDA levels and decreasing SOD activity and GSH levels in the testicular tissue of rats. It also affects the release of reproductive hormones by altering the hypothalamic-pituitary axis. Favipiravir administration decreases the expression of genes that induce sperm capacitation and acrosome reaction and decreases sperm quality by causing changes in testicular histoarchitecture. Study results reveal that favipiravir treatment negatively affects testes and semen quality.
Pürinerjik Reseptör Antagonisti A438079’un Sıçanlarda LPS’nin Neden Olduğu Böbrek Hasarı Üzerindeki Koruyucu Etkileri
(2023) Celep, Nevra Aydemir; Erbaş, Elif; Kara, Adem; Özkanlar, Seçkin; Ulas, Nergis
Sepsis, organ disfonksiyonu ve yüksek mortalite ile ilişkili önemli bir klinik problemdir. Bu pürinerjik reseptörün sepsiste böbrek fonksiyon bozukluğu üzerindeki etkisini araştırmak için intraperitoneal LPS (Lipopolisakarit) enjeksiyonu ile sıçan modeli oluşturuldu. Çalışma Kontrol, A438079, LPS, LPS ve LPS+A438079 gruplarından oluşmaktaydı. Çalışma gruplarına LPS (8 mg/kg) enjeksiyonunu takiben A438079 (15 mg/kg) uygulandı (i.p). Daha sonra anestezi altında sakrifiye edilen ratlardan analizler için kan ve böbrek dokuları toplandı. Histopatolojik değerlendirmelerde A438079’un sepsisin neden olduğu hasarı azalttığı görüldü. LPS+A438079 grubuna ait serum örneklerinin kreatinin ve BUN (Blood Urea Nitrogen) konsantrasyonlarının LPS grubuna ait serum örneklerine göre daha düşük olduğu belirlendi. Böbrek dokularında AQP2, Bcl-2, Kaspaz-3 ve NfkB-p65 protein ekspresyonları western blot ile değerlendirildi. Öte yandan, A438079 ile tedavi edilen gruplarda bu proteinlerin ifadesinde azalma olduğu gözlendi. Sonuç olarak, A438079, LPS’nin neden olduğu akut böbrek hasarında enflamatuar yanıtı azaltabilir ve böbrek fonksiyon bozukluğunu iyileştirebileceği belirlenmiştir.
Silymarin Reduces Paclitaxel-Induced Lung Damage Via Down-Regulating P2x7r Expression and Inhibiting Inflammation and Apoptosis in the Rats
(Univ Agriculture, Fac Veterinary Science, 2024) Celep, Nevra Aydemir; Erbas, Elif; Kara, Hulya; Kara, Adem
This study explores the impact of silymarin, a potent natural flavonoid with robust antioxidant properties, on paclitaxel-induced lung injury. Paclitaxel, a widely used chemotherapeutic agent for cancer, is known for its adverse effects on various organs. The research involved four groups: the Control group (6 animals) received oral saline; the SIL group (6 animals) received 200 mg/kg silymarin orally for 10 days; the PAX group (6 animals) received intraperitoneal paclitaxel (2 mg/kg) for 5 days; and the PAX+SIL group (6 animals) received both treatments. All groups were sacrificed on the 10th day. Histopathological analysis revealed pathological changes in the lung tissue of the PAX group. Immunopositivity of Bax, iNOS, Nf-kB, and IL-6 antibodies was higher in the PAX group compared to silymarin-treated groups. Conversely, Bcl2 and MUC1 immunopositivity was lower in the PAX group but higher in silymarintreated groups. Silymarin administration decreased IL-6, Caspase-3, P2x7 and NFkB p65 immunoreactivity, while increased Bcl-2 immunoreactivity. Protein expression levels of IL-6, Caspase-3, P2x7, and NF-KB were higher in the PAX group but decreased in the PAX+SIL group. Bcl-2 protein expression was lower in the PAX group but higher in the PAX+SIL group. Additionally, antioxidant enzyme levels increased, while MDA levels decreased in the PAX+SIL group. In conclusion, the findings indicate that silymarin effectively ameliorated paclitaxel-induced lung damage, highlighting its potential therapeutic role in mitigating chemotherapy-related side effects.