Browsing by Author "Cicek, Mustafa"

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Can Mecsina Hemostopper, Which Has a Cytotoxic Effect on Mcf-7 Cells, Be Considered an Anticarcinogenic Agent Due to Its Immunological Properties
(2024) Cicek, Mustafa; Tumer, Mehmet Kemal; Turgut, Alpgiray
Aim: Mecsina is used as a hemostatic agent such as Ankaferd and Tranexamic acid. To struggle with breast cancer, which is a serious public health issue, new, effective, and less toxic therapeutic agents are needed. Hence, it is aimed to compare and evaluate the effects of Mecsina and Ankaferd, both of which contain natural biomolecules in their structure, and synthetic Tranexamic acid on MCF-7 cells. Material and Method: For this study, MCF-7 immortalized cell lines were commercially purchased. The cells, 5000 cells per flask for each different dose group, were distributed to the 9 groups (mecsina 1:1, 1:2, 1:10, 1:50, 1:100, 1:200, 1:500, distilled water administered negative and control without any administration). Cytotoxicity, ELISA cytokine levels were evaluated, and flow cytometric analyzes were performed for each group using the XTT analysis method, after 24 hours of incubation. Results: A significant difference was observed between different doses of drug administration groups of Mecsina Hemostopper hemostatic agent in MCF-7 cells (p<0.001). Besides, cytokine levels were found to be significantly higher than those of other possible therapeutic agents. Conclusion: Mecsina Hemostopper has been found to have anti-tumoral activity in MCF-7 cancer cell lines by producing a hemostatic effect.
The Genotoxic, Hepatotoxic, Nephrotoxic, Haematotoxic and Histopathological Effects in Rats After Aluminium Chronic Intoxication
(Sage Publications Inc, 2013) Geyikoglu, Fatime; Turkez, Hasan; Bakir, Tulay Ozhan; Cicek, Mustafa
Aluminium (Al) is used in water purification and is also present in several manufactured foods and medicines. Al is known to induce a broad range of physiological, biochemical and behavioural dysfunctions in laboratory animals and humans. This investigation was carried out to investigate the effects of subchronic exposure to Al (as AlCl3) in rats. Sprague-Dawley rats were randomly separated into two groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with Al (as AlCl3, 5mg/kg body weight) intraperitonally for 10 weeks. Animals were killed and blood samples were analyzed for blood serum alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) enzyme activities and creatinine, urea (U) and uric acid (UA) levels for evaluating hepatotoxicity and nephrotoxicity. Blood parameters including red blood cells (RBCs), haemoglobin (Hb) concentration, haematocrit (Ht), platelets (PLTs) and white blood cells (WBCs) were compared between control and experimental group to assess haematoxicity. In order to determine the genotoxicity, the number of micronucleated hepatocytes (MNHEPs) was counted in isolated hepatocytes. In addition, histological alterations in liver and kidney samples were investigated. After exposure with Al, the enzymatic activities of ALP, AST, ALT and LDH, and the levels of U and UA significantly increased. RBC, WBC, PLT, Hb and Ht revealed significant decreases in experimental group compared to the control. AlCl3 caused a significant increase in MNHEPs. Furthermore, severe pathological damages were established in both liver and kidney samples. Subchronic exposure to low doses of Al can produce serious dysfunctions in rat blood, liver and kidney, and exposure to this metal can result in greater damages.