Browsing by Author "Eraslan, Ersen"

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Over İskemi-Reperfüzyon Hasarında Sefarantinin Terapötik Potansiyeli: Rat Over Torsiyon-Detorsiyon Modelinden Bulgular
(2025) Eraslan, Ersen; Tanyeli, Ayhan; Akdemir, Fazile Nur Ekinci; Guler, Mustafa Can; Erdoğan, Derya Güzel; Polat, Elif; Yazıcı, Şahin
Bu çalışma, Sefarantin’in (CEP)’in, sıçanlarda torsiyon–detorsiyon ile oluşturulan over iskemi– reperfüzyon (İ/R) hasarına karşı koruyucu etkisini ve histopatolojik hasar ile oksidatif stres ve inflamasyon göstergeleri üzerindeki etkisini değerlendirmiştir. Yirmi dört dişi Sprague-Dawley sıçanı rastgele üç deney grubuna ayrılmıştır: sham, T/D ve CEP 10 mg/kg. Çalışma, over doku örneklerinde malondialdehit (MDA), miyeloperoksidaz (MPO) ve süperoksit dismutaz (SOD) gibi oksidatif stres biyobelirteçleri ile tümör nekroz faktör-alfa (TNF-α) ve interlökin-1 beta (IL-1β) gibi proinflamatuar mediyatörleri ölçmüştür. T/D grubu, yüksek MDA ve MPO seviyeleri ile düşük SOD aktivitesi ile karakterize önemli oksidatif stres ve yüksek TNF-α ve IL-1β seviyelerinin gösterdiği artmış inflamatuar yanıtlar sergiledi. CEP uygulaması bu zararlı etkileri azalttı, oksidatif stresi ve inflamatuar sitokinseviyeleriniönemli ölçüdeazaltırken SODaktivitesinirestoreetti. CEP, over İ/R hasarında oksidatif stres ve inflamasyonu etkili bir şekilde azaltmıştır. Elde edilen preklinik bulgular, CEP’nin potansiyel terapötik etkisini desteklemektedir, ancak klinik uygulamalar için ileri çalışmalara ihtiyaç vardır.
Therapeutic Effects of Apocynin on Ovarian Ischemia-Reperfusion Induced Lung Injury
(Taylor & Francis Ltd, 2022) Tanyeli, Ayhan; Erdogan, Derya Guzel; Comakli, Selim; Polat, Elif; Guler, Mustafa Can; Eraslan, Ersen; Doganay, Songul
Ovarian ischemia-reperfusion (I-R) injury may damage remote organs, including the lungs. We investigated whether apocynin, a NADPH oxidase inhibitor, might protect against ovarian I-R induced apoptosis in the lungs of rats. Bilateral ovarian I-R was induced for 3 h, then apocynin was applied at two concentrations. Lung tissue was evaluated using spectrophotometric and immunohistochemical methods. We found that I-R increased total oxidant status (TOS), oxidative stress index (OSI) and myeloperoxidase (MPO) levels, and immunostaining of nuclear factor kappa-B (NF-kappa B), light chain 3B (LC3B), interleukin 1-beta (IL-1 beta), caspase-3 and tumor necrosis factor-alpha (TNF-alpha), but decreased superoxide dismutase (SOD) values. Apocynin application to I-R injured rats enhanced recovery of lung tissue oxidants and improved both histology and frequency of apoptosis.
Therapeutic Potential of Irisin in Ovarian Torsion-Detorsion: Mitigating Oxidative Stress and Inflammatory Response
(Marmara Univ, Inst Health Sciences, 2025) Guler, Mustafa Can; Bircan, Burak; Eraslan, Ersen; Akdemir, Fazile Nur Ekinci; Yazici, Sahin; Erdogan, Derya Guzel; Tanyeli, Ayhan
Objective: Ovarian torsion (OT) is a critical gynecological emergency marked by partial or complete rotation of the ovary around its ligamentous supports, resulting in impaired blood flow and potential ischemic injury or necrosis if not treated promptly. This study explores the potential of irisin in mitigating ischemia-reperfusion (I/R) injury following ovarian torsion-detorsion (T/D), with a focus on oxidative stress and inflammatory mediators. Methods: Twenty-four female Sprague-Dawley rats were allocated into three groups: Sham, T/D, and irisin 10 mu g/kg. Oxidative stress markers-total oxidant status (TOS), oxidative stress index (OSI), malondialdehyde (MDA), and myeloperoxidase (MPO)-were measured to assess tissue damage. Antioxidant parameters, including superoxide dismutase (SOD) activity and total antioxidant status (TAS) were evaluated. Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) were analyzed to assess systemic inflammation and irisin's modulatory role. Results: The T/D group showed elevated MPO and MDA levels relative to the sham group (p Conclusion: The findings support the potential of irisin as a therapeutic agent for mitigating oxidative stress and inflammation in ovarian I/R injury. Irisin may offer clinical benefits in preserving ovarian function during conditions related to T/D.
Urapidil Alleviates Ovarian Torsion Detorsion Injury Via Regulating Oxidative Stress, Apoptosis, Autophagy, and Inflammation
(Mashhad University of Medical Sciences, 2021) Guler, Mustafa Can; Tanyeli, Ayhan; Erdogan, Derya Guzel; Eraslan, Ersen; Comakli, Selim; Polat, Elif; Doganay, Songul
Objective(s): This study aimed to determine anti-inflammatory, antioxidant, and antiapoptotic properties of urapidil (Ura) against ovarian torsion detorsion (T/D) injury in rats. Materials and Methods: 40 female Wistar albino rats were grouped as sham, T/D, T/D+dimethyl sulfoxide (DMSO), T/D+Urapidil (Ura) 0.5 mg/kg (low dose), and T/D+Urapidil (Ura) 5 mg/kg (high dose) groups. In treatment groups, Ura was administered intraperitoneally just before detorsion. Biochemical parameters (TAS, TOS, MDA, MPO, and SOD) and immunohistochemical (IL-1 beta, TNF-alpha, NF-kappa B, LC3B, and Caspase-3) analyzes were performed. Results: In the T/D group, OSI and MPO levels were elevated significantly while TAS values decreased compared with the sham group. A significant difference occurred in the low dose treatment group in TAS and OSI levels compared with the T/D group. In the high dose treatment group, significant elevation in TAS but reduction in OSI and MDA levels were observed compared with the T/D group. Immunohistochemical staining resulted in IL-1 beta, TNF-alpha, NF-kappa B, LC3B, and caspase-3 immunopositivity in the T/D group, while Ura treatment decreased those parameters. Intensive congestion and hemorrhage were observed in the T/D group, but contrary to this, treatment groups had alleviated congestion and hemorrhage. Conclusion: These results suggest that Ura demonstrated protective effects against ovarian T/D injury via anti-oxidative, anti-inflammatory, and anti-apoptotic features.