Browsing by Author "Gormez, Arzu"

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The Alternative Methods for Disinfection of E. Coli
(Amer Inst Physics, 2016) Yetim, Tuba; Gormez, Arzu; Gurkok, Sumeyra
Recently, advanced oxidation processes have gained significant interest for bacterial inactivation. In the present study, the efficacy of sonolysis, photocatalysis and sonophotocatalysis was evaluated for disinfection of Escherichia coli O157:H7 strain. Sonophotocatalysis proved to be the most effective disinfection methods by generating greater amount of (OH)-O-center dot radical.
Antibacterial Activity and Chemical Composition of Essential Oil Obtained from Nepeta Nuda Against Phytopathogenic Bacteria
(Taylor & Francis Inc, 2013) Gormez, Arzu; Bozari, Sedat; Yanmis, Derya; Gulluce, Medine; Agar, Guleray; Sahin, Fikrettin
The increase in agricultural production can be possible with the discovery and utilization of chemical compounds for plant disease control. However, indiscriminate use of chemical compounds against microorganisms causes many negative side effects on environment and mammalian health. Therefore, we aimed to find a new natural antimicrobial product from medicinal plants against phytopathogenic bacteria. In this study, the essential oil (EO) of Nepeta nuda was isolated by the hydrodistillation method and the chemical composition of the EO was analyzed by gas chromatography-mass spectrometry (GC-MS), as its biological activity was connected to its content. Then, the antibacterial property of EO was evaluated against twenty phytopathogenic bacteria. Nepeta contains 4a,7,7a-nepetalactone (18.10%), germacrene (15.68%), elemol (14.38%), -caryophyllene (8.81%), spathulenol (6.14%) and cubenol (5.10%) as major components. In conclusion, the present study is valuable for future investigations on controlling plant pathogenic bacteria.
Antibacterial Activity of Essential Oils Extracted from Satureja Hortensis Against Selected Clinical Pathogens
(American Institute of Physics, 2017) Gormez, Arzu; Yanmis, Derya; Bozari, Sedat; Gurkok, Sumeyra
The antibiotic resistance of pathogenic microorganisms has become a worldwide concern to public health. To overcome the current resistance problem, new antimicrobial agents are extremely needed. The aim of the present study was to evaluate the antibacterial activity of Satureja hortensis essential oils against seven clinical pathogens. Chemical compositions of hydro distillated essential oils from S. hortensis were analyzed by GS-MS. The antibacterial activity was investigated against Corynebacterium diphtheria, Salmonella typhimurium, Serratia plymuthica Yersinia enterocolitica, Y. frederiksenii, Y. pseudotuberculosis and Vibrio cholerae by the use of disc diffusion method and broth micro dilution method. The minimum inhibitory concentration (MIC) values of essential oils were found as low as 7.81 mu g/mL. Notably, essential oils of S. hortensis exhibited remarkable antimicrobial activities against the tested clinical pathogens. The results indicate that these essential oils can be used in treatment of different infectious diseases.
Carbonate and Silicate Dissolving Bacteria Isolated from Home-Made Yogurt Samples
(Academia Brasileira de Ciências, 2021) Orhan, Furkan; Demirci, Abdullah; Gormez, Arzu
In the current study, twenty-eight bacterial strains were isolated from home-made yogurt samples from Agri Province, Turkey. The bacterial strains were identified by conventional and molecular techniques. Among the twenty- eight isolates, seventeen isolates were identified according to the 16 5 rDNA region and determined to belong to five different genus including Sphingomonas (8 isolates), Burkholderia (5 isolates), Lactobacillus (2 isolates), Lactococcus (1 isolate), Staphylococcus (1 isolate). In this study, the presence of Burkholderia in home-made yogurt samples were reported for the first time, whereas Sphingomonas was detected for the second time. We also investigated the carbonate (CaCO3 and MgCO3) and silicate CCaSiO3 and MgSiO3) dissolving potential of seventeen bacterial isolates. Among these seventeen bacterial isolates, fifteen bacterial isolates have CaCO (3)-dissolving and 10 bacterial isolates have MgCO3-dissolving potential. The silicates dissolution ability was relatively less than that of carbonates dissolving. We observed that six bacterial isolates have CaSiO3 and only two bacterial isolates have MgSiO3 dissolution abilities. In conclusion, this work clearly shows the diversity of bacteria existing in fermented cow milk samples in Agri Province, Turkey, which could be considered as valuable sources for lactic acid bacteria (LAB) isolation and further probiotic potential.
Characterization and Biotechnological Application of Protease from Thermophilic Thermomonas Haemolytica
(Springer Heidelberg, 2020) Oztas Gulmus, Ebru; Gormez, Arzu
In this study, it was aimed to determine the ability to produce protease enzyme of Thermomonas haemolytica isolated from geothermal Nenehatun hot spring in Turkey and utilization of this enzyme in the detergent industry to remove protein stains. The protease-producing strains were screened from hot springs, and a potential strain was identified as T. haemolytica according to morphological, physiological and biochemical characteristics and sequence of 16S rRNA gene. Maximum protease activity was observed at 55 degrees C and pH 9.0 at 72 h of incubation. Activity was very stable between 50 and 65 degrees C and pH 8.0-10.0, respectively. The enzyme activity was significantly inhibited by PMSF and partly inhibited by EDTA, EGTA, SDS, and urea. Some divalent metal ions such as Ca2+, Mg2+, and Mn2+ increased the enzyme activity, while Zn2+ and Cu2+ decreased. Michaelis-Menten constant (K-m) and maximum velocity (V-max) values were calculated by Lineweaver-Burk plot as 125 EU/ml and 1262 mg/ml, respectively. The biochemical characterization of the protease obtained from T. haemolytica was performed and applied on the blood and grass-stained fabrics with detergent to evaluate the stain removal performance of the enzyme. It was observed that the application of detergent with enzyme was more effective than the detergent without enzyme to clean up the stained fabrics. This is the first report of characterization of the protease of T. haemolytica. According to results obtained from this study, this new strain is a promising candidate for industrial applications in production of detergent.
Characterization of the Potential Cytotoxicity of Klebsiella pneumoniae Isolated from Colorectal Cancer Patients Against a Normal Human Fibroblast Model
(Amer Chemical Soc, 2025) Kaci, Fatma Necmiye; Daglioglu, Cenk; Gormez, Arzu
Colorectal cancer is one of the most common types of cancer worldwide, with a multifactorial digestive pathology. Evidence has suggested that gut microbial dysbiosis is connected to the development of colorectal cancer by generating cancer cell-conducive microenvironments. Nevertheless, the relationship between colorectal cancer pathogenesis and microorganisms has not been fully clarified to date. Here, we addressed this issue and determined the cancer-causing potential of the culture filtrate and proteins of Klebsiella pneumoniae on healthy cells. In this study, the culture filtrate and total proteins of K. pneumoniae isolated from patients with colorectal cancer were investigated to determine their cytotoxic effects against the normal human fibroblast PCS-201-012 cell model. As a result of the isolation procedure, three different K. pneumoniae strains (named Kp1, Kp2, and Kp3) were obtained from biopsy samples. Their 16S rRNA gene sequences were submitted to the GenBank database under the accession numbers MK156319, MK156320, and MK156321, respectively. The WST-8 and hemolysis tests were performed to examine the exacerbating effect of these strains on normal cells. The apoptosis-inducing ability of the isolated strains was characterized based on a combination of several techniques: determination of caspase-3 activity, JC-1 mitochondrial assay, and flow cytometry-based FITC-Annexin-V/PI double staining. Moreover, the expression profiles of four candidate genes (APC, SMAD, KRAS, TP53), which play important roles in the development of colorectal cancer, were analyzed by the qRT-PCR method. Cell biology experiments demonstrated that the culture filtrate and proteins of the related strains clearly cause cell death in normal human fibroblasts due to increased inflammatory response and necrosis. Furthermore, the culture filtrates and proteins led to a decrease in the expression of tumor suppressor genes TP53, SMAD, and APC and an increase in the expression of the KRAS oncogene, emphasizing the tumorigenicity of the strains in colorectal cancer. These results revealed that K. pneumoniae strains are capable of triggering cytotoxicity in normal human fibroblast cells.
Chemical Composition and Antibacterial Activity of Essential Oils of Two Species of Lamiaceae Against Phytopathogenic Bacteria
(Polskie Towarzystwo Mikrobiologow-Polish Society of Microbiologists, 2015) Gormez, Arzu; Bozari, Sedat; Yanmis, Derya; Gulluce, Medine; Sahin, Fikrettin; Agar, Guleray
In this study, we aimed to determine chemical composition and antibacterial activities of Satureja hortensis and Calamintha nepeta against to 20 phytopathogenic bacteria causing serious crop loss. The essential oils of S. hortensis and C. nepeta were isolated by the hydrodistillation method and the chemical composition of the essential oils were analyzed by GC-MS. The antibacterial properties of the essential oils were evaluated against 20 phytopathogenic bacteria through Disc diffusion assay and micro dilution assay. The results revealed that the essential oils of S. hortensis and C. nepeta have significant antibacterial activity. Furthermore, the findings of the study are valuable for future investigations focusing on the alternative natural compounds to control plant diseases.
Composition and Bioactivity of Essential Oil from Stachys Macrostachya (Wend.) Briq
(Acad Brasileira de Ciencias, 2021) Karaoglan, Esen Sezen; Gormez, Arzu; Yilmaz, Bilal; Kaci, Fatma Necmiye; Ozgen, Ufuk
Stachys species belonging to Lamiaceae family have been used for medicinal purposes since ancient times. The aim of the present study was to investigate the chemical compositions and antibacterial, anti-tyrosinase activities of the essential oil of Stachys macrostachya. The essential oil was prepared by hydrodistillation method using a Clevenger-type apparatus and chemical composition was determined by gas chromatography (GC). The antibacterial activity of essential oil was performed by the disc diffusion and microdilution broth method against five Gram-positive and two Gram-negative bacteria. The tyrosinase inhibitory activity was evaluated by minor modifications of Masuda's method. According to the results of GC analyses, twenty-three compounds were identified representing 91.9% of the total volatile composition. The main compounds were germacrene D (12.2%), globulol (10.9%), alpha-pinene (9.7%), and valencene (7.6%). The present study showed that the tested essential oil of S. macrostachya exhibited antibacterial activity against Acinetobacter baumannii (MIC 62.50 mu g/mL) and tyrosinase inhibition activity (IC50 22.86 +/- 0.82 mu g/mL). These results suggest that the essential oil could be exploited as a potential source of natural antimicrobial agents of this bacterium as well as tyrosinase inhibitors.
Determination of Genotoxic and Antigenotoxic Effects of Peltigera Canica by the Bacterial Reverse Mutation Assays
(Elsevier Sci Ltd, 2013) Gormez, Arzu; Karadayi, Mehmet; Gulluce, Medine; Baris, Ozlem; Orhan, Furkan; Sahin, Fikrettin
Determination of Total Xylanase Activities of Various Thermophilic Bacteria
(2021) Gormez, Arzu; Ulucay, Orhan; Ozic, Cem
Enzymes, which have important metabolic functions in living organisms, make important contributions to human beings by using them for different purposes in many areas such as economy, food, agriculture, and industry as part of the daily lives. Today, enzymes, whose production and usage purposes, are increasing, were generally obtained from plant, animal, and microorganisms. In this study; the enzyme producing isolates and their total xylanase activities of various thermophilic bacteria (Bacillus coagulans, Bacillus licheniformis, Bacillus subtilis, Bacillus sp. and Geobacillus kaustophilus) isolated from previously hot springs in Eastern and Southeastern Anatolia regions were determined. In the enzyme activity of the isolates, 47 isolates showed xylanase activity less than 0.1 U/ml, 31 isolates were between 0.1 U/ml and 0.2 U/ml. However, 5 isolates of B. subtilis [BTX3 (0.206 U/ml), BTX6 (0.286 U/ml), BTX22 (0.2 U/ml), BTX27 (0.203 U/ml) and BTX32 (0.206 U/ml)] were exhibited highest activity for xylanase enzyme production.
The Effect of Novel Β-Lactam Derivatives Synthesized from Substituted Phenethylamines on Resistance Genes of MRSA Isolates
(Springer Nature, 2024) Yildirim, Merve; Ozgeris, Bunyamin; Gormez, Arzu
This study focuses on the activity of previously reported imine and beta-lactam derivatives against methicillin-resistant Staphylococcus aureus (MRSA) isolates. The presence of mecA and blaZ genes in the isolates was determined, and the minimum inhibitory concentration (MIC) values were determined based on the antibacterial activity against these isolates. Active compounds were selected and their ability to act against resistant isolates in vitro was determined. Concurrently, biochemical (nitrocefin) and molecular (qRT-PCR) tests were used to investigate the ability of the compounds to induce resistance genes in MRSA isolates. The cytotoxicity of the compounds on human dermal fibroblasts (HDF) was investigated. The MIC values of compounds (10) and (12) against MSSA and MRSA isolates were 7.81 and 15.62 mu g ml-1, respectively. The most active compounds were identified as (10) and (12), and it was observed that the isolates did not develop resistance to these compounds in vitro. These compounds were found to inhibit beta-lactamase, reduce the expression of resistance genes, and exhibit reduced HDF cell toxicity in a dose-dependent manner. According to the findings of the study, it can be concluded that these compounds show promise as hits with an interesting mechanism of action for further chemical modifications to develop new MRSA inhibitors.
Effect of Punica Granatum L. Peel Extract on Phytopathogenic Bacteria
(National Inst Science Communication & Information Resources-niscair, 2022) Ruzgar, Damla; Efe, Derya; Gormez, Arzu
Pathogens, animals and weeds, among the biotic factors, cause yield losses between 20 to 40% in agricultural production. Therefore, much research has focused on minimizing these product losses and improving product yield. Mainly, synthetic agents and antibiotics have been used for a long time against plant pathogenic bacteria. But there is a search for alternative natural compounds because of undesirable effects on the environment. For this purpose, in this study, the pomegranate peels extract was prepared in different concentrations (600-100 mu g/mL) and evaluated against plant pathogenic bacteria (Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. carotovorum, Pseudomonas corrugate, Pseudomonas mediterranea, Rhizobium radiobacter, and Xanthomonas campestris). According to the results, the antibacterial effect of pomegranate peel extract was observed for P. mediterranea, P. carotovorum subsp. carotovorum, and R. radiobacter. The largest zone diameter (10.4 mm) and lowest MIC value (100 mu g/mL) were observed against P. mediterranea. Therefore, it is possible to conclude that pomegranate peel extracts is effective against some phytopathogenic bacteria. Also, according to the study results, it is deduced that waste materials can be used as an alternative to synthetic drugs and thus, prevent toxic substance pollution with completely organic waste.
For Biotechnological Applications: Purification and Characterization of Recombinant and Nanoconjugated Xylanase Enzyme from Thermophilic Bacillus Subtilis
(Elsevier, 2022) Ulucay, Orhan; Gormez, Arzu; Ozic, Cem
This study aimed to produce and nanoconjugated the xylanase enzyme of Bacillus subtilis BTX6 (MH101286) isolated from geothermal hot springs in Diyadin, Agri (Davud). Within the scope of this study, the 1,4-beta-endo xylanase (GH11 family) free enzyme from B. subtilis was cloned, recom-binantly expressed in E. coli after that nanoconjugated by encapsulation with the Anadoluca method. Then, the recombinantly produced enzyme and the nanoconjugated enzyme were char-acterized. According to the results, the optimum activity of both recombinant enzyme and nanoconjugated xylanase enzyme were determined as pH 7.0. Considering the optimal tempera-ture values, it was determined that the recombinant enzyme display optimum activity at 68 degrees C, while the nanoconjugated enzyme shows the best activity at 75 degrees C. The molecular weight of the recombinantly produced enzyme was measured as 71 kDa. In the enzyme activity measurements, the recombinant enzyme was determined as 1803 U/mg., and the activity of the nanoconjugated enzyme was determined as 1898 U/mg. Some metal ions such as MgSO4, CuSO4, CaCl2, ZnSO4, and FeSO4 increased the activity of recombinant and nanoconjugated enzymes. The Km value for the recombinant enzyme was 2.298 (mM), the Vmax value was 5.691 (U/mg.). Nanoconjugated enzyme the enzyme increased Km (2.298-2.402 mM) and Vmax (5.691-6.195 U/mg.) values. As a result, it was concluded that the cloning and nanoconjugated xylanase enzyme methods preferred in this study can be used effectively to improve enzyme activity in industrial processes.
Identification and Characterization of Novel Thermophilic Bacteria from Hot Springs, Erzurum, Turkey
(Springer, 2020) Oztas Gulmus, Ebru; Gormez, Arzu
The aim of present study was the isolation and characterization of thermophilic bacteria from three different hot springs in Erzurum, Turkey. For this purpose, 85 bacteria were isolated and characterized by ERIC-PCR genomic fingerprinting and classical identification methods such as morphological, physiological and biochemical characteristics. According to the results, 29 bacterial isolates with different band profiles were analyzed by 16S rRNA gene sequencing and identified as belonging to the genus of Bacillus, Pseudomonas, Silanimonas, Thermomonas and Thauera. This is the first report on the isolation of Silanimonas lenta, Thauera sp. and Thermomonas haemolytica from Turkey Hot Springs. The amylase, lipase and protease enzyme production potentials of the isolates were 80%, 91.25% and 81.25%, respectively. Moreover, 56.47% of the isolates (48) were able to produce all of these enzymes. Therefore, the results of the study indicated that these bacteria and their enzymes can be used as a source of industrial enzymes.
Identification of Bacteria Producing Red Pigments and Their Application in the Textile Industry
(Springer Heidelberg, 2025) Geyik, Merve Simsek; Efe, Derya; Gormez, Arzu
In this study, bacterial strains previously isolated and maintained in a culture collection were evaluated for their pigment production capabilities. The pigments produced by the bacteria were using the methanol extraction method and characterized by thin-layer chromatography, UV-Vis, and Fourier transform infrared spectroscopy (FTIR). In addition, the potential use of these pigments as textile dyes in silk fabrics was investigated. Four of the 270 isolates screened (AS-54, AS-55, AS-67, and AS-75) showed superior pigment production. These isolates were further identified using both classical and molecular methods. Based on the analysis of the 16S rRNA gene region, AS-54, AS-55, and AS-67 were identified as Serratia plymuthica and, AS-75 as Serratia marcescens. Characterization results showed that the pigments exhibited a red coloration, indicating prodigiosin. The dyeing of silk fabrics with these pigments resulted in various shades of pink, highlighting their potential as textile dyes. These results suggest that pigments derived from these bacterial isolates hold great promise for applications in the textile industry.
Identification, Characterization and Hydrolase Producing Performance of Thermophilic Bacteria: Geothermal Hot Springs in the Eastern and Southeastern Anatolia Regions of Turkey
(Springer, 2022) Ulucay, Orhan; Gormez, Arzu; Ozic, Cem
In the last two decades, researchers have increasingly focused on the rich microorganism-based diversity of natural hot spring sources to explore the benefits of thermophiles in industrial and biotechnological fields. Within the scope of this study, a total of 83 thermophilic Bacilli strains were isolated from 7 different geothermal hot springs (at temperatures ranging between 40 and 85 degrees C) located in the Eastern and Southeastern Anatolia Regions of Turkey. The physiological, morphological, biochemical and molecular properties of the isolates were determined. As a result of the 16S rRNA gene sequence analysis, 5 different species (Bacillus licheniformis, Bacillus sp., Bacillus subtilis, Geobacillus kaustophilus, and Weizmannia coagulans,) were identified. B. licheniformis and B. subtilis were the most frequently encountered species among those obtained from the researched hot spring sources. Phylogenetic analysis was conducted to evaluate the phylogenetic relationships of the isolated species. The results showed that there was no significant difference between the groups and the bacteria in terms of the locations or optimum temperatures of the isolates. The bacterial isolates were screened for amylase, cellulase, lipase and protease hydrolytic enzyme activities. The hydrolytic enzyme production potentials among the isolates were identified in 68 (82%) isolates for amylase, 34 (41%) for cellulase, 69 (83%) for lipase and 73 (88%) for protease. All isolates were found to have at least one or more extracellular enzyme activities. Additionally, it was determined that 27 of the existing isolates (32.8%) were able to produce all of the aforementioned hydrolytic enzymes.
Investigation of the Antibacterial Activity, Cytotoxicity, and Effects on Enamel and Composite Resin of Mouthwash Formulations Prepared with Three Different Boron Compounds
(Springer Heidelberg, 2025) Mehmethanoglu, Iklima; Ozakar, Emrah; Ozakar, Rukiye Sevinc; Ozakar, Nurcan; Ruzgar, Damla; Gormez, Arzu
Objective: This study investigated the use of oral rinse solutions formulated with boron-containing compounds, known for their antibacterial activity, as an alternative to chlorhexidine (CHX). Material and method: Boron nitride (BN), boric acid (BA) and sodium borate (SB) were used in the study. BN was used in nanosuspension (BN-NS) due to its low solubility in water. BA and SB were also prepared and used in solution form (BA-S, SB-S). Antibacterial activity against cariogenic bacteria was evaluated using agar well diffusion and microdilution techniques, and antibiofilm activity was evaluated using the crystal violet method. Cytotoxicity in human gingival cell lines (HGF-1) was evaluated using MTT and LDH assays. Surface hardness was measured with Vicker's Microhardness tester and surface roughness was measured with a profilometer. CHX was used as the control group. Results: BN-NS, BA-S, and SB-S showed antibacterial and antibiofilm activity against all cariogenic bacteria. BN-NS and SB-S demonstrated much lower cytotoxicity compared to CHX, while BA-S exhibited similar cytotoxicity. SB-S caused an increase in enamel surface hardness (p = 0.017), whereas no significant changes were observed in surface roughness or hardness in the other groups (p > 0.05). Conclusion: BN-NS and SB-S showed antibacterial effects and antibiofilm activity on all tested cariogenic bacteria. BN-NS and SB-S showed lower cytotoxicity than CHX, and their effects on enamel and composite resin surfaces were similar to the control group. Further studies are needed to support the use of BN-NS and SB-S as alternatives to CHX.
Isolation and Characterization of Novel Chitinolytic Bacteria
(Amer Inst Physics, 2016) Gurkok, Sumeyra; Gormez, Arzu
Chitin, a linear polymer of beta-1,4-N-acetylglucosamine units, is one of the most abundant biopolymers widely distributed in the marine and terrestrial environments. It is found as a structural component of insects, crustaceans and the cell walls of fungi. Chitinases, the enzymes degrading chitin by cleaving the beta-(1-4) bond, have gained increased attention due to their wide range of biotechnological applications, especially for biocontrol of harmful insects and phytopathogenic fungi in agriculture. In the present study, 200 bacterial isolates from Western Anatolia Region of Turkey were screened for chitinolytic activity on agar media amended with colloidal chitin. Based on the chitin hydrolysis zone, 13 isolates were selected for further study. Bacterial isolates with the highest chitinase activity were identified as Acinetobacter calcoaceticus, Arthrobacter oxydans, Bacillus cereus, Bacillus megaterium, Brevibacillus reuszeri, Kocuria erythromyxa, Kocuria rosea, Novosphingobium capsulatum, Rhodococcus bratislaviensis, Rhodococcus fascians and Staphylococcus cohnii by MIS and BIOLOG systems. The next aims of the study are to compare the productivity of these bacteria quantitatively, to purify the enzyme from the most potent producer and to apply the pure enzyme for the fight against the phytopathogenic fungi and harmful insects.
A Mini Report on Palynological and Antibacterial Tests of Four Propolis Samples from Different Regional Origins
(2020) Bayram, Sinan; Kaci, Fatma Necmiye; Bayram, Nesrin Ecem; Gormez, Arzu
In this study, palynological and antibacterial tests of propolis samples from Iran-Turan (IP1 and IP2),Mediterranean (MP), and Europe-Siberia (EP) phytogeographical regions were performed. The pollens ofApiaceae, Asteraceae, Betulaceae, Boraginaceae, Brassicaceae, Campanulaceae, Caryophyllaceae,Fabaceae, Fagacee, Lamiaceae, Poaceae, Pinaceae, Ranunculaceae, Rosaceae, Salicaceae andScrophulariaceae taxa were found in the palynological analysis. Gram negative and Gram positivebacteria were used to determine in vitro antibacterial activities of the propolis samples. The most potentinhibitory effect against the target microorganisms was obtained from IP1. The most resistant strains wereBurkholderia cepacia, Citrobacter freundii and Streptococcus pneumoniae for all the propolis samples.But, the antibacterial activity levels of the samples were different from each other. These results indicatethat propolis can be assessed in different areas such as cosmetic, medicine and food as an antimicrobialagent.
Punica Granatum L. Kabuklarının Etanol Ekstraktlarının Sitotoksik ve Antibakteriyel Aktivitelerinin Değerlendirilmesi
(2021) Kaci, Fatma Necmiye; Gormez, Arzu; Rüzgar, Damla; Efe, Derya
Çalışmada, nar kabuğu ekstraktlarının antibakteriyel aktivitesi ve güvenilirliğini kanıtlamak amacıyla insan dermalfibroblast primer hücreleri üzerindeki sitotoksisitesinin araştırılması amaçlanmıştır. Nar kabuklarının etanol özütlerininantibakteriyel etkinliği Acinetobacter baumannii, Escherichia coli, Staphylococcus aureus MRSA ATCC 67101,Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus pneumoniae, Enterococcusfaecalis, Staphylococcus epidermidis, Burkholderia cepacia, Bacillus cereus, Citrobacter freundii ve Cedecea neteri’ye karşı disk difüzyon ve mikrodilüsyon yöntemleriyle test edildi. Aynı zamanda, ekstraktın insan dermal fibroblast hücreleriüzerindeki etkileri de WST-8 deneyi ile belirlendi. Çalışmanın sonuçlarına göre; nar kabuklarının etanol özütü, A. baumannii, E. coli, S. aureus MRSA ATCC 67101, P. aeruginosa, E. faecalis, S. epidermidis, B. cepacia, B. cereus, C. freundii ve C.neteri’ye karşı 100 ila 500 μg $mL^{-1}$ arasında değişen oranlarda MİK değerleri ile potansiyel olarak etkiliydi. Çalışmadaekstraktın K. pneumoniae, S. aureus and S. pneumoniae’ye karşı inhibisyon aktivitesi gözlenmedi. Aynı zamanda Punicagranatum L. kabuğunun etanol özütü, dermal fibroblast hücrelerine karşı da herhangi bir sitotoksisiteye sahip değildi.Çalışmanın sonuçlarına göre, ekstraktın antibakteriyel özelliğe sahip olduğu ve toksik etkisi olmaması nedeniyle de birçokendüstriyel üründe doğal koruyucu bir bileşen olarak kullanılabileceği önerilmektedir.