Browsing by Author "Karatas, Omer"

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Expression and Prognostic Value of Ing3 in Advanced Laryngeal Squamous Cell Carcinoma
(2023) Gündoğdu, Betül; Karatas, Omer; Tatar, Arzu; Şahin, Abdulkadir; Barlak, Neslisah; Kuşdemir, Gülnur; Gümüş, Rasim
Objectives: Laryngeal squamous cell carcinomas (LSCC) is one of the most common aggressive neoplasms of the head and neck region. There is a significant need for identification of successful and accurate prognostic markers to better estimate the clinical outcomes for LSCC patients. In this study, we aimed at analyzing the differential expressions of inhibitor growth (ING) family members and to evaluate the prognostic values of deregulated ING genes in LSCC. Methods: We investigated the relative expressions of ING genes in laryngeal tumor-normal tissue pairs in the mRNA level using quantitative real-time polymerase chain reaction and relative expression of ING3 in the protein level using Western Blot analysis. Results: The rate of genetic alterations of ING3 was relatively higher in head and neck cancer including LSCC. ING3 expression was significantly upregulated in LSCC tissue samples in both mRNA and protein level. Higher expression of ING3 was also correlated with poor disease-free survival of patients with head and neck cancer. Conclusions: Our findings assigned an oncogenic feature for ING3 in laryngeal cancer with a significant upregulation detected in advanced cases and suggested a vital prognostic potential for ING3.
Larenks Kanseri ve Mikrorna’lar Arasındaki İlişki
(2020) Kaya, Murat; Karatas, Omer
Larenks kanseri (LKa) en sık görülen baş boyun kanseridir ve bu kanser türünün tüm kanserler içerisindeki oranı yaklaşık %1-%2,5 civarındadır. LKa’nın %95 kadarlık bir kısmı skuamöz hücrelerden köken almaktadır. LKa oluşumunun birçok nedeni olmakla birlikte sigara ve alkol kullanımı en büyük risk faktörleri arasında yer almaktadır. Son yirmi yılda birçok alandaki gelişmelere paralel olarak LKa tedavisinde de önemli ilerlemeler olmasına rağmen, ileri LKa’lı olgularda klinik sonuç istenilen seviyelere ulaşamamıştır. MikroRNA (miRNA)’lar endojen olarak oluşturulan, yaklaşık 18-24 nükleotid uzunluğunda tek zincirli ve kodlamayan kısa RNA’lardır. Birçok hastalığın oluşmasında ve ilerlemesinde kritik rol oynayan miRNA’ların, LKa’da da önemli mekanizmaları etkileyerek hastalığın oluşması ve ilerlemesine neden olduğu yapılan çok sayıdaki çalışma ile ortaya çıkarılmıştır. Birçok tümör baskılayıcı ve onkogen özellikli miRNA’nın ifadelerinin değişerek LKa kanser sürecini etkilediği yapılan çeşitli çalışmalarla anlaşılmıştır. Bu derlemede LKa ve miRNA arasındaki ilişkiyi ortaya çıkaran güncel araştırmaların sonuçları bir araya getirilerek irdelenmiş ve miRNA’ların LKa üzerindeki etkisi tartışılmıştır.
Mex3d Is an Oncogenic Driver in Prostate Cancer
(Wiley, 2021) Shao, Longjiang; Wang, Jianghua; Karatas, Omer; Ittmann, Michael
Background Prostate cancer (PCa) is the most common visceral malignancy and the second leading cause of cancer deaths in US men. The two most common genetic alterations in PCa are expression of the TMPRSS2/ERG (TE) fusion gene and loss of the PTEN tumor suppressor. These genetic alterations act cooperatively to transform prostatic epithelium but the exact mechanisms involved are unclear. Methods Microarray expression analysis of immortalized prostate epithelial cells transformed by loss of PTEN and expression of the TE fusion revealed MEX3D as one of the most highly upregulated genes. MEX3D expression in prostate cancer was examined in patient samples and in silico. In vitro and in vivo studies to characterize the biological impact of MEX3D were carried out. Analysis of the TCGA PanCancer database revealed TCF3 as a major target of MEX3D. The induction of TCF3 by MEX3D was confirmed and the biological impact of TCF3 examined by in vitro studies. Results MEX3D is expressed at increased levels in prostate cancer and is increased by decreased PTEN and/or expression of the TE fusion gene and drives soft agar colony formation, invasion and tumor formation in vivo. The known oncogenic transcription factor TCF3 is highly correlated with MEX3D in prostate cancer. MEX3D expression strongly induces TCF3, which promotes soft agar colony formation and invasion in vitro. Conclusions Loss of PTEN and expression of the TE fusion gene in prostate cancer strongly upregulates expression of MEX3D and its target TCF3 and promotes transformation associated phenotypes via this pathway.