Antioxidant Biochemical and Larvicidal Activity of Cyclamen Hederifolium Extracts

Abstract

This study aimed to determine the total phenolic and flavonoid contents of Cyclamen hederifolium ethanolic, methanolic and acetone extracts, and antioxidant activities of them, to investigate the potential safety/toxicity risks of the different parts and doses of the C. hederifolium ethanolic extracts using enzymes (ALT. ALP, and CRE), and the larvicidal effect of water extract against houseflies (Musca domestica) and mosquitoes (Culex pipiens). In vitro antioxidant activity of the extracts determined using DPPH and. ABTS assays. Total phenolic and flavonoid contents of the extract represented as mg GAE/g-extract and mg QE/g-extract, respectively. For enzyme assay, the blood of five different groups of rats were analysed on days 0, 15, and 30. The highest value with the lowest IC50 value in the DPPH test was obtained from the ethanolic extract of the above ground (AG) (0.83 0.00 ug/mL. The highest value with the lowest IC50 value in the ABTS test was obtained from the methanolic extract of the underground (UG) (0.16 0.00.tg/mL), Statistically significant increases in AG 1% group and significant decreases in UG 1% group were observed in ALT and ALP values (p < 0.05), The AG extract was found to be more toxic against M. domestica (30,45 + 2.78%) and Cr. pipiens (83.33 + 0.00%). Considering the ALT and ALP measurements, the increase in the values in AG 1% group over time indicates liver damage. It seems that UG 1% extract reduces liver enzyme levels, and it has increased larvicidal effect over time.