The Antioxidant and Genotoxic Activities of Na2b4o7•10h2o in Vitro

Abstract

Boron is known as a trace element for animals and humans. Sodium borates are used in some cosmetics, detergents, and chemicals of photography, adhesives and textile compounds. It was aimed to evaluate the antioxidant potentials and genotoxic risk of sodium borate (Na(2)B4O(7)center dot 10H(2)O) on human whole blood cultures in the extent of this study (n=5). Na(2)B4O(7)center dot 10H(2)O was administrated at wide range concentrations (0-1280 mu g/ml) to cultured human whole blood samples. The sister chromatid exchange (SCE), micronucleus (MN) and 8-oxo-2-deoxyguanosine (8-OH-dG) assays were performed to determine genotoxic effect. In addition, total antioxidant capacity (TAC) and total oxidative stress (TOS) levels were measured as biochemical parameters. Our results clearly demonstrated that all tested concentrations of Na(2)B4O(7)center dot 10H(2)O were not mutagenic. Moreover, Na(2)B4O(7)center dot 10H(2)O showed antioxidant activities especially at low concentrations (<40 mg/L) and the determined TOS levels did not change at all the concentrations of Na(2)B4O(7)center dot 10H(2)O. As a result, our data clearly demonstrated that Na(2)B4O(7)center dot 10H(2)O is non-genotoxic and it has a remarkable antioxidant potential in vitro.