Genc, HayriyeKalin, RamazanKoksal, ZeynepSadeghian, NastaranKocyigit, Umit M.Zengin, MustafaOzdemir, Hasan2026-03-262026-03-2620161422-006710.3390/ijms171017362-s2.0-84992092066https://doi.org/10.3390/ijms17101736https://hdl.handle.net/20.500.14901/2181Gulcin, Ilhami/0000-0001-5993-1668; Zengin, Mustafa/0000-0002-0243-1432; Sadeghian, Nastaran/0009-0004-2966-9231; Kocyigit, Umit Muhammet/0000-0001-8710-2912; Köksal, Zeynep/0000-0003-4800-9461; Genç Bilgiçli, Hayriye/0000-0001-6909-316Xbeta-Lactams are pharmacologically important compounds because of their various biological uses, including antibiotic and so on. beta-Lactams were synthesized from benzylidene-inden derivatives and acetoxyacetyl chloride. The inhibitory effect of these compounds was examined for human carbonic anhydrase I and II (hCA I, and II) and acetylcholinesterase (AChE). The results reveal that beta-lactams are inhibitors of hCA I, II and AChE. The Ki values of beta-lactams (2a-k) were 0.44-6.29 nM against hCA I, 0.93-8.34 nM against hCA II, and 0.25-1.13 nM against AChE. Our findings indicate that beta-lactams (2a-k) inhibit both carbonic anhydrases (CA) isoenzymes and AChE at low nanomolar concentrations.eninfo:eu-repo/semantics/openAccessCarbonic AnhydraseAcetylcholinesteraseBeta-Lactam2-AzetidinoneEnzyme InhibitionEnzyme PurificationArticle