Guler, Mustafa CanTanyeli, AyhanErdogan, Derya GuzelEraslan, ErsenComakli, SelimPolat, ElifDoganay, Songul2026-03-262026-03-2620212008-38662008-387410.22038/ijbms.2021.57196.127362-s2.0-85109321812https://doi.org/10.22038/ijbms.2021.57196.12736https://hdl.handle.net/20.500.14901/1651Doganay, Songul/0000-0002-1730-1331; Guler, Mustafa Can/0000-0001-8588-1035; Çomakli, Selim/0000-0002-8744-7686; Güzel Erdoğan, Derya/0000-0002-7618-5043Objective(s): This study aimed to determine anti-inflammatory, antioxidant, and antiapoptotic properties of urapidil (Ura) against ovarian torsion detorsion (T/D) injury in rats. Materials and Methods: 40 female Wistar albino rats were grouped as sham, T/D, T/D+dimethyl sulfoxide (DMSO), T/D+Urapidil (Ura) 0.5 mg/kg (low dose), and T/D+Urapidil (Ura) 5 mg/kg (high dose) groups. In treatment groups, Ura was administered intraperitoneally just before detorsion. Biochemical parameters (TAS, TOS, MDA, MPO, and SOD) and immunohistochemical (IL-1 beta, TNF-alpha, NF-kappa B, LC3B, and Caspase-3) analyzes were performed. Results: In the T/D group, OSI and MPO levels were elevated significantly while TAS values decreased compared with the sham group. A significant difference occurred in the low dose treatment group in TAS and OSI levels compared with the T/D group. In the high dose treatment group, significant elevation in TAS but reduction in OSI and MDA levels were observed compared with the T/D group. Immunohistochemical staining resulted in IL-1 beta, TNF-alpha, NF-kappa B, LC3B, and caspase-3 immunopositivity in the T/D group, while Ura treatment decreased those parameters. Intensive congestion and hemorrhage were observed in the T/D group, but contrary to this, treatment groups had alleviated congestion and hemorrhage. Conclusion: These results suggest that Ura demonstrated protective effects against ovarian T/D injury via anti-oxidative, anti-inflammatory, and anti-apoptotic features.eninfo:eu-repo/semantics/closedAccessAutophagyCaspase-3DetorsionNF-Kappa BOvarianTorsionUrapidilArticle