Koksal, ZeynepKalin, RamazanGulcin, IlhamiOzdemir, Hasan2026-03-262026-03-2620181094-29121532-238610.1080/10942912.2018.14241972-s2.0-85052545656https://doi.org/10.1080/10942912.2018.1424197https://hdl.handle.net/20.500.14901/2557Gulcin, Ilhami/0000-0001-5993-1668The objective of this study was to determine the in vitro inhibition effects of seven commonly used pesticides including 2,4-d-acid dimethylamine, fenoxaprop-p-ethyl, glyphosate isopropylamine, haloxyfop-p-methyl, cypermethrin, -cyhalothrin, and dichlorvos on the peroxidase purified from turnip (Brassica rapa L.) and black radish (Raphanus sativus L.) using 4-amino benzohydrazide affinity column chromatography. The purification factors for the turnip and black radish peroxidases were found to be 263.29-fold (with a yield of 12.89%) and 36.20-fold (with a yield of 6.90%), respectively. Among these compounds, -cyhalothrin showed the strongest inhibitory effect against turnip peroxidase (K-i: 1.23 x 10(-2)+/- 0.21 x 10(-2)mM) as noncompetitive inhibition. On the other hand, cypermethrin demonstrated the highest inhibition effect against black radish peroxidase (K-i: 2.14 x 10(-2)+/- 0.08 x 10(-2)mM) as competitive inhibition.eninfo:eu-repo/semantics/openAccessPlant PeroxidaseRadish (Raphanus Sativus)Turnip (Brassica Rapa)PesticidesEnzyme InhibitionArticle