Koksal, ZeynepKalin, RamazanGulcin, IlhamiOzdemir, HasanAtasever, Ali2026-03-262026-03-2620161094-29121532-238610.1080/10942912.2015.10764572-s2.0-84960539825https://doi.org/10.1080/10942912.2015.1076457https://hdl.handle.net/20.500.14901/2160Many macrocyclic lactones, including avermectins, are known to be used as a veterinary drug, agricultural pesticides, and insecticides. Lactoperoxidase (EC 1.11.1.7) is one of the peroxidases found in milk. Lactoperoxidase has a natural host defense system against micro-organisms and a natural antimicrobial system. In this study, some macrocyclic lactones, including emamectin-benzoate, doramectin, eprinomectin, abamectin, moxidectin-vetranal, and ivermectin were investigated for in vitro inhibitory effects on the bovine lactoperoxidase enzyme, which was purified using amberlite CG-50 H+ resin and sepharose 4B-L-tyrosine-sulphanamide affinity chromatography 344.6-fold, with a yield of 61.1% and a specific activity of 39.11 EU/mg protein. Emamectin-benzoate, doramectin, eprinomectin, abamectin, moxidectin-vetranal, and ivermectin are also known strong antiparasitary properties. In this study, we demonstrated that avermectins have strong lactoperoxidase inhibitory effects. Of these, the emamectin-benzoate was shown to have the most inhibiting effect against lactoperoxidase with Ki value of 6.82 +/- 2.60 mu M.eninfo:eu-repo/semantics/closedAccessLactoperoxidaseEnzyme PurificationEnzyme InhibitionAvermectinsAffinity ChromatographyArticle