Gelen, VolkanYesildag, AliAkarsu, Serkan AliKara, HulyaTekiner, DenizKara, Adem2026-03-262026-03-2620252048-717710.1002/fsn3.708932-s2.0-105022628904https://doi.org/10.1002/fsn3.70893https://hdl.handle.net/20.500.14901/2895Diabetes, a metabolic disease characterized by high blood sugar, causes damage to many organs in the body. Accordingly, the present study investigates the effects of silver nanoparticle-loaded 18 beta-glycyrrhetinic acid (AgNP+18 beta-GA) on P2X7 receptor and ER stress-mediated NLRP3 inflammasome activation in testicular tissue. 42 Wistar-Albino rats aged 6 months were used. The rats were divided into the following seven groups: Control, AgNP+18 beta-GA100, DM, DM-18 beta-GA100, DM-AgNP+18 beta-GA50, DM-AgNP+18 beta-GA100, and DM-AgNP. After the experiment, sperm samples obtained from the rats were evaluated. Oxidative stress markers, serum IL-1 beta, and TNF-alpha levels were determined in the testicular tissue samples taken separately. In addition, Caspase-1, NLRP3, P2X7, Caspase-3, NF-kappa B, IRE1, ATF6, and CHOP protein expression levels were evaluated using Western blot analysis of histopathological examinations. Specifically, the most significant decrease was observed in NLRP3, Caspase-1, P2X7, and NF-kappa B levels in the DM-AgNP+18 beta-GA100 group (p < 0.05). About sperm parameters, while sperm motility and viability significantly decreased in diabetic groups (p < 0.05), a significant improvement in these values was observed in the AgNP+18 beta-GA applied groups (p < 0.05). AgNP+18 beta-GA improved sperm morphology and histopathological damage in diabetes-induced testicular damage, as well as inhibited P2X7 receptor and endoplasmic reticulum stress-mediated NLRP3 inflammasome activation.eninfo:eu-repo/semantics/openAccess18 Beta-Glycyrrhetinic AcidAgNPsDiabetesER-StressNLRPRatTestisArticle