Tanyeli, AyhanErdogan, Derya GuzelComakli, SelimPolat, ElifGuler, Mustafa CanEraslan, ErsenDoganay, Songul2026-03-262026-03-2620221052-02951473-776010.1080/10520295.2022.20363682-s2.0-85125263844https://doi.org/10.1080/10520295.2022.2036368https://hdl.handle.net/20.500.14901/2017Guler, Mustafa Can/0000-0001-8588-1035; Güzel Erdoğan, Derya/0000-0002-7618-5043; Doganay, Songul/0000-0002-1730-1331Ovarian ischemia-reperfusion (I-R) injury may damage remote organs, including the lungs. We investigated whether apocynin, a NADPH oxidase inhibitor, might protect against ovarian I-R induced apoptosis in the lungs of rats. Bilateral ovarian I-R was induced for 3 h, then apocynin was applied at two concentrations. Lung tissue was evaluated using spectrophotometric and immunohistochemical methods. We found that I-R increased total oxidant status (TOS), oxidative stress index (OSI) and myeloperoxidase (MPO) levels, and immunostaining of nuclear factor kappa-B (NF-kappa B), light chain 3B (LC3B), interleukin 1-beta (IL-1 beta), caspase-3 and tumor necrosis factor-alpha (TNF-alpha), but decreased superoxide dismutase (SOD) values. Apocynin application to I-R injured rats enhanced recovery of lung tissue oxidants and improved both histology and frequency of apoptosis.eninfo:eu-repo/semantics/closedAccessApocyninApoptosisIschemiaLung InjuryOxidative DamageRatsReperfusionArticle