Browsing by Author "Celikezen, Fatih Caglar"

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The Antioxidant and Genotoxic Activities of Na2b4o7•10h2o in Vitro
(Parlar Scientific Publications (p S P), 2015) Celikezen, Fatih Caglar; Turkez, Hasan; Aydin, Elanur
Boron is known as a trace element for animals and humans. Sodium borates are used in some cosmetics, detergents, and chemicals of photography, adhesives and textile compounds. It was aimed to evaluate the antioxidant potentials and genotoxic risk of sodium borate (Na(2)B4O(7)center dot 10H(2)O) on human whole blood cultures in the extent of this study (n=5). Na(2)B4O(7)center dot 10H(2)O was administrated at wide range concentrations (0-1280 mu g/ml) to cultured human whole blood samples. The sister chromatid exchange (SCE), micronucleus (MN) and 8-oxo-2-deoxyguanosine (8-OH-dG) assays were performed to determine genotoxic effect. In addition, total antioxidant capacity (TAC) and total oxidative stress (TOS) levels were measured as biochemical parameters. Our results clearly demonstrated that all tested concentrations of Na(2)B4O(7)center dot 10H(2)O were not mutagenic. Moreover, Na(2)B4O(7)center dot 10H(2)O showed antioxidant activities especially at low concentrations (<40 mg/L) and the determined TOS levels did not change at all the concentrations of Na(2)B4O(7)center dot 10H(2)O. As a result, our data clearly demonstrated that Na(2)B4O(7)center dot 10H(2)O is non-genotoxic and it has a remarkable antioxidant potential in vitro.
Cytogenetic and Oxidative Alterations After Exposure of Cultured Human Whole Blood Cells to Lithium Metaborate Dehydrate
(Springer, 2016) Celikezen, Fatih Caglar; Togar, Basak; Ozgeris, Fatma Betul; Izgi, Mehmet Sait; Turkez, Hasan
Boron compounds have an ability of supporting antioxidant properties in human and animal tissues. Lithium metaborate dihydrate (LiBO2 center dot 2H(2)O; LMD) is commonly used in nonlinear optic materials, cellular phones and pagers. But, there are limited data on the genotoxic and antioxidant effects of LMD in cultured human whole blood cells. The aim of this study was to evaluate for the genotoxicity and antioxidant/oxidant activity of LMD on human whole blood lymphocytes (n = 5). LMD was applied at various concentrations (0-1,280 A mu g/ml) to cultured blood samples. Antioxidant/oxidant activity was evaluated by measuring the total oxidant status (TOS) and total antioxidant capacity levels. Micronuclei and chromosomal aberration tests were used in genotoxicity studies. Our results clearly revealed that all tested concentrations of LMD were found to be non-genotoxic when compared to that of the control group. In addition, LMD exhibited antioxidant activities at low concentrations. In addition the TOS levels were not changed at all concentrations of LMD. Consequently, our results clearly demonstrated that LMD is non-genotoxic and it has an important antioxidant potential in vitro.
Cytotoxic and Antioxidant Properties of Essential Oil of Centaurea Behen L. in Vitro
(Springer, 2019) Celikezen, Fatih Caglar; Hayta, Sukru; Ozdemir, Ozlem; Turkez, Hasan
Centaurea species of Asteraceae family are widely use in traditional medicine. Despite wide medicinal use of Centaurea sp., there is limited knowledge concerning Centaurea behen toxicity. Therefore, in this study, it is aimed to determine cytotoxic and oxidative effects of essential oil of C. behen on human blood cell cultures. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays were performed to determine cytotoxic effects. In addition, total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative potentials. The results indicated that all tested concentrations of essential oil of C. behen were cytotoxic and led to decreases of cell viability in both assays. Besides, C. behen led to significant increases of TOS levels and decreases of TAC levels. As a conclusion, the present study showed for the first time the cytotoxic and oxidant effects of essential oil of C. behen on cultured human whole blood cells.
Determination of the in Vitro Cytotoxic Activities of Several Coumarin Derivatives on Neuroblastoma Cell Lines with in Silico Inhibitory Effects on Cdk9, Vegfr2 and Egfr Proteins and ADME Studies
(Wiley, 2025) Celikezen, Fatih Caglar; Sarac, Kamuran; Seyhan, Ercan; Aslan, Mehmet Enes; Oner, Sena; Turkez, Hasan
Due to their stable nature and medical applicability properties, coumarin derivatives have fascinated medicinal chemists in the discovery of novel therapeutics. In this study, the cytotoxic/anticancer properties of some newly synthesized coumarin derivatives were aimed at designing, synthesizing, and examining cultured human neuroblastoma cells. Moreover, molecular docking studies were carried out to determine the potential mechanism. In addition, ADMET properties were evaluated to examine the drug-likeness of newly designed coumarin derivatives. To detect the cytotoxic action of compounds, 3-(4,5-dimethylthiazol-2-yl)-2,5 2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays were carried out. In addition, Hoechst 33258 staining was used to detect abnormal nuclear structures. In silico, the estimates for all compounds (3a-3c) used in the study revealed that they possessed desirable physicochemical properties for bioavailability. The results of our study showed that all tested compounds exhibited remarkable cytotoxic effects on human neuroblastoma cell lines (p < 0.05). Additionally, among the compounds tested, 3a and 3c showed selective effects on neuroblastoma cells effectively at all tested concentrations. However, it was found that the selective feature of 3b, unlike the others, was concentration-dependent. Our findings clearly showed that novel coumarin derivatives exerted potent and selective anticancer effects. Results of molecular docking studies were in parallel with in vitro studies. Unlike the majority of hybrid coumarin derivatives reported in anticancer research, the present study introduces minimalist, heteroatom-free coumarins bearing bulky aliphatic substituents. These compounds demonstrated selective cytotoxicity against SH-SY5Y neuroblastoma cells and a favorable multi-target binding profile, highlighting a distinct hydrophobic volume-based SAR. As a result, the obtained data exhibited that all used molecules may be good multitarget drug alternatives for the treatment of neuroblastoma.
DNA Damaging and Biochemical Effects of Potassium Tetraborate
(Excli Journal Managing Office, 2014) Celikezen, Fatih Caglar; Turkez, Hasan; Togar, Basak; Izgi, Mehmet Sait
Potassium tetraborate (PTB) is a product resulting from the controlled reaction of potassium hydroxide, water and boric acid (BA). It is used in many areas of industry such as disinfectant, detergent and treatment of contact lenses. PTB is one of the boron compounds which is most commonly used in many areas of industry although very limited information is available concerning its toxicity. Therefore, in this study, it is aimed to determine genetic and biochemical effects of PTB in human blood cell cultures (n=4). PTB was added into culture tubes at various concentrations (0-1280 mu g/ml). Micronucleus (MN) and chromosomal aberration (CA) tests were performed for genotoxic damage influences estimation. In addition, biochemical parameters (total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine oxidative effects. The results indicated that all tested concentrations of PTB were found to be non-genotoxic. In addition, low concentrations (1.25, 2.5 and 5 mu g/ml) of PTB caused increases of TAC levels. Furthermore, all concentrations of PTB were not changed the TOS levels in cultured human blood cells. Based on these results, in this study it has been reported for the first time that PTB is not genotoxic and it increases the antioxidant capacity in human peripheral blood lymphocytes.
In Vitro Assessment of Genotoxic and Oxidative Effects of Zinc Borate
(Taylor & Francis Ltd, 2014) Celikezen, Fatih Caglar; Turkez, Hasan; Togar, Basak
Zinc borate is used as flame retardant for plastics and cellulose fibers, paper, rubber, and textiles. Despite its wide industrial use, there is limited information concerning its toxicity. The aim of the present study was to investigate the concentration dependence (0-280mg L-1) of its genotoxic activity on cultured human lymphocytes by using sister chromatid exchange and chromosomal aberration assays. Total antioxidant capacity and the extent of oxidative stress were also determined. Zinc borate was found to be non-genotoxic at all tested concentrations. It exhibited antioxidant activity at concentrations lower than 40mg L-1, and total oxidative stress levels were not changed at any applied concentration of zinc borate.
In Vitro Evaluation of Selective Cytotoxic Activity of Chaerophyllum Macropodum Boiss. on Cultured Human SH-SY5Y Neuroblastoma Cells
(Springer, 2022) Celikezen, Fatih Caglar; Turkez, Hasan; Firat, Mehmet; Arslan, Mehmet Enes; Oner, Sena
Neuroblastoma is the most common solid tumor in children. New treatment approaches are needed because of the harmful side effects and costs of the methods used in the treatment of neuroblastoma. Medicinal and aromatic plants are important for new treatment approaches due to their minimal side effects and economic advantages. Therefore, the present study was carried out to examine the cytotoxic effect of Chaerophyllum macropodum extract on human neuroblastoma (SH-SY5Y) and fibroblast (HDFa) cell lines. 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase release (LDH) assays were used to determine the cytotoxic effect of C. macropodum. The extracts were analyzed for their phenolic content by HPLC-PDA. Major components were determined as 63.600% o-coumaric acid, 15.606% catechine hydrate, 8.713% rosmarinic acid, 4.376% clorogenic acid, and 3.972% salicylic acid. The obtained results from cytotoxicity testing revealed that C. macropodum exerted a significant cytotoxic effect on human neuroblastoma cells at all tested concentrations (p < 0.05). But it did not lead to any cytotoxic potential on human fibroblasts. As a result, the obtained data clearly revealed C. macropodum exerted a selective cytotoxic action on neuroblastoma cells for the first time.
Synthesis of the 3,5-Diphenyl and Cytogenetic and Oxidative Alterations After Exposure of Cultured Human Whole Blood Cells
(Taylor & Francis AS, 2017) Akbas, Esvet; Celikezen, Fatih Caglar; Turkez, Hasan; Ozdemir, Ozlem; Ruzgar, Adem; Ergan, Erdem; Sahin, Ertan
The 3,5-diphenyl-1H-pyrazole was obtained by condensation reaction of dibenzoylmethane and thiosemicarbazide in acetic acid under conventional heating and microwave irradiation method. The structure of the 3,5-diphenyl-1H-pyrazole confirmed by IR, H-1, and C-13 NMR and X-ray diffraction and the geometry optimization was carried out using density functional theory (DFT) methods at B3LYP/6-31G, 6-31G(d), 6-31G(d, p), 6-311G(d, p), 6-311G(2d, 2p), 6-31+G(d, p), 6-311++G(d, p) levels. In addition, cytotoxic and oxidative effects were investigated in cultured human peripheral blood cells.