* Akademik Arşiv

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Erzurum Müstakbel Planı
(1939) Erzurum Müstakbel Planı, 1939 (J. H. Lambert Arşivi, IFEA)
Görsel sergiden alındığı için sergiye referans verilmelidir. Künyesi şu şekilde: “Murat Tülek, Jean-François Pérouse, Gülşen Özaydın, Y. Barış Göğüş, Saadet Tuğçe Tezer. 1930’larda Eskişehir’i Planlamak: Fransız Plancı J.H. Lambert’in Bilinmeyen Katkısı Sergisi, Eskişehir Büyükşehir Belediyesi Kent Müzeleri Kompleksi, Eskişehir, Aralık 2021.”
1939 Lambert Erzurum Plani
(1939) Lambert
1960`lı yıllar Erzurum Krokisi
(1960) Eski Eserler ve Doğan Kuban Raporu
Citation - WoS: 8
Citation - Scopus: 9
The Effect of Laurel Leaf Extract Against Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo in Cultured Rat Hepatocytes
(Inst Medical Research & Occupational Health, 2011) Turkez, Hasan; Geyikoglu, Fatime
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a very toxic environmental pollutant that raises great public concern about its impact on human health. Recent studies indicate that laurel leaf extract exhibits antioxidant properties that can counter the toxic effects of certain compounds in the liver. The aim of this study was to assess how effective LE is against the toxicity of TCDD in a primary culture of rat hepatocytes. The extract (50 mg L-1, 100 mg L-1, and 200 mg L-1) was added to cultures alone or with TCDD (1.61 mg L-1 and 3.22 mg L-1) for 48 hours. Cell viability was measured using the [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and the lactate dehydrogenase (LDH) cytotoxicity assay, while oxidative damage was assessed by measuring total antioxidant capacity (TAC) and total oxidative stress (TOS). DNA damage was also analysed using the micronucleus (MN) assay of the cultured hepatocytes. TCDD alone lowered, and laurel extract had no effect on cell viability. TCDD also increased TOS and significantly decreased TAG. It significantly increased the frequency of micronucleated hepatocytes in a dose-dependent manner. In cultures exposed to LE alone, TOS did not change and TAG significantly increased in a dose-dependent manner. Added to TCDD, laurel countered its toxic effects and showed protective effects against TCDD-mediated DNA damage. This points to the therapeutic potential of laurel against TCDD toxicity in the liver.
Citation - WoS: 11
Citation - Scopus: 12
The in Vitro Effects of Dermotocarpon Intestiniforme (A Lichen) Extracts Against Cadmium Induced Genetic and Oxidative Damage
(Foundation Environmental Protection & Research-FEPR, 2012) Guner, Adem; Turkez, Hasan; Aslan, Ali
Cadmium (Cd) is one of the most harmful heavy metals and influences important metabolic processes causing serious damages in many organs. It is well known that Cd could provoke generation of reactive oxygen species (ROS) and DNA damage. Some antioxidants (such as vitamin C, vitamin E and N-acetyl-L-cysteine) have been used as protectors against cadmium-induced toxicity. On the other hand, lichens have long been investigated for biological activities; mainly antimicrobial and antioxidant activities. In this study, we aimed to determine whether D. intestiniforme aqueous extracts (25 and 50 ppm) conferred a protection against cadmium chloride (CdCl2) (30 ppm) induced genetic and oxidative damage in human whole blood cultures. Biochemical parameters (total antioxidant capacity [TAC] and total oxidative status [TOS]) were examined to determine oxidative effects. The micronucleus (MN) test was used for analysing genotoxic influences. In addition nucleer division indeks (NDI) was used to determine cytotoxicty. Oxidative damage by CdCl2 in erythrocytes decreased with application of lichen extracts. Similarly, the positive effect of lichen aqueous extracts in decreasing the incidence of MN in comparison with an unprotected level was attained when cultures were treated simultaneously with CdCl2 and the extracts (P<0.05). The findings of this study firstly revealed that D. intestiniforme modulated Cd-induced genetic and oxidative damage in human blood cultures due to its antioxidant and/or detoxifying nature.
Citation - WoS: 22
Citation - Scopus: 22
Ameliorative Effect of Supplementation with L-Glutamine on Oxidative Stress, DNA Damage, Cell Viability and Hepatotoxicity Induced by 2,3,7,8-Tetrachlorodibenzo in Rat Hepatocyte Cultures
(Springer, 2012) Turkez, Hasan; Geyikoglu, Fatime; Yousef, Mokhtar I.; Celik, Kubra; Bakir, Tulay O.
The most potent of the dioxins, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is a persistent and ubiquitous environmental contaminant. And the health impact of exposure to TCDD is of great concern to the general public. Recent data indicate that l-glutamine (Gln) has antioxidant properties and may influence hepatotoxicity. The objective of the present study was undertaken to explore the effectiveness of Gln in alleviating the hepatotoxicity of TCDD on primary cultured rat hepatocytes. Gln (0.5, 1 and 2 mM) was added to cultures alone or simultaneously with TCDD (0.005 and 0.01 mM). The hepatocytes were treated with TCDD and Gln for 48 h. Then cell viability was detected by [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and lactate dehydrogenase (LDH) release, while total antioxidant capacity (TAC), total glutathione (TGSH) and total oxidative stress (TOS) levels were determined to evaluate the oxidative injury. The DNA damage was also analyzed by liver micronucleus assay (MN) and 8-oxo-2-deoxyguanosine (8-OH-dG). The results of MTT and LDH assays showed that TCDD decreased cell viability but not l-glutamine. TCDD also increased TOS level in rat hepatocytes and significantly decreased TAC and TGSH levels. On the basis of increasing doses, the dioxin in a dose-dependent manner caused significant increases of micronucleated hepatocytes (MNHEPs) and 8-OH-dG as compared to control culture. Whereas, in cultures exposured with Gln alone, TOS levels were not changed and TAC and TGSH together were significantly increased in dose-dependent fashion. The presence of Gln with TCDD modulated the hepatotoxic effects of TCDD on primary hepatocytes cultures. Noteworthy, Gln has a protective effect against TCDD-mediated DNA damages. As conclusion, we reported here an increased potential therapeutic significance of l-glutamine in TCDD-mediated hepatic injury for the first time.
Citation - WoS: 38
Citation - Scopus: 38
Xanthoria Elegans (Link) (Lichen) Extract Counteracts DNA Damage and Oxidative Stress of Mitomycin C in Human Lymphocytes
(Springer, 2012) Turkez, Hasan; Aydin, Elanur; Aslan, Ali
Several lichen species have been used for medicinal purposes throughout the ages, and they are reported to be effective in the treatment of different disorders including ulcer and cancer. It is revealed that lichens may be easily accessible sources of natural drugs and possible food supplements after their safety evaluations. The main objective in this study was to evaluate the roles of aqueous extracts of Xanthoria elegans (at 25, 50 and 100 mu g/ml) upon mitomycin C (MMC; at 10(-7) M) induced genotoxic and oxidative damages in cultured human lymphocytes. X. elegans were collected from the Erzurum and Artvin provinces (in Turkey) during August 2010. After the application of MMC and X. elegans extract (XEE), separate and together, human whole blood cultures were assessed by four genotoxicity end-points including chromosomal aberration, micronucleus, sister chromatid exchange (SCE) and 8-oxo-2-deoxyguanosine (8-OH-dG) assays. In addition, biochemical parameters [total antioxidant capacity (TAC) and total oxidative stress (TOS)] were examined to determine oxidative effects. According to our results, the frequencies of cytogenetic endpoints and 8-OH-dG levels were significantly increased by MMC compared with controls in human peripheral lymphocytes. MMC caused oxidative stress by altering TAC and TOS levels. On the contrary, XEE led to increases of TAC level without changing TOS level. XEE had no genotoxic effect. Furthermore, our findings revealed that MMC induced increases in the mean frequencies of four genotoxic indices were diminished by XEE in dose dependent manner, indicating its protective role towards cells from MMC exerted injury. In conclusion, the results obtained in the present study indicate for the first time that XEE is a potential source of natural antigenotoxicants.
Citation - WoS: 77
Citation - Scopus: 91
Investigation of Neutron Shielding Properties Depending on Number of Boron Atoms for Colemanite, Ulexite and Tincal Ores by Experiments and Fluka Monte Carlo Simulations
(Pergamon-Elsevier Science Ltd, 2012) Korkut, Turgay; Karabulut, Abdulhalik; Budak, Gokhan; Aygun, Bunyamin; Gencel, Osman; Hancerliogullari, Aybaba
Am-241-Be source and three samples including different amounts of boron atoms per unit volume called colemanite, ulexite and tincal were used in total macroscopic cross section experiments. Also FLUKA Monte Carlo code was used to simulate total macroscopic cross sections, absorbed doses and deposited energies by low energy neutron interactions. Besides half value layers of samples were calculated and compared to paraffin. As a result, ascending concentration of boron atoms can enhance neutron shielding property of samples. (C) 2011 Elsevier Ltd. All rights reserved.
Sağlık Hizmetleri Talebi: Erzurum İlinde Bir Araştırma
(2012) Kaynak, Selahattin; Yaylalı, Muammer; Karaca, Zeynep
Sağlık hizmetleri sektörü gelişme potansiyeli geniş olan bir sektördür. Her bireyin sağlık hizmetleri ile karşılaşma ihtimalinin yüksek olması, sağlık hizmetlerine ayrılan kaynakların toplumsal refah için kullanılan kaynakların önemli bir bölümünü teşkil etmesi ve dışsallıkları çok yüksek olan bir sektör olması nedeniyle bu sektöre olan talebin araştırılması önem arz etmektedir. Sektörün bu önemine istinaden hazırlanan çalışmanın amacı sağlık hizmetlerinin talebinde etkili olan faktörleri belirlemektir. Bu amaç doğrultusunda anket verileri kullanılarak sağlık hizmetleri talebini içeren bir logit modeli SPSS ve LIMDEP paket programları ile tahmin edilmiştir. Analiz sonucunda tüketicilerin sağlık hizmeti talep etme olasılıklarının gelir, yaş ve ailedeki birey sayısı ile doğru orantılı olduğu tespit edilmiştir. Ayrıca ekonomik faktörler, sağlık personelinin tutum ve davranış ile hastanelerin fiziksel ortamı gibi grup değişkenlerinin ortalaması 0,05 anlamlılık düzeyinde genel ortalamadan istatistiki açıdan anlamlı derecede farklı olduğu tespit edilmiştir.
İthal Ham Petrol Fiyatlarının Türkiye’deki Makroekonomik Aktiviteler Üzerindeki Etkisi
(2012) Lebe, Fuat; Yaylalı, Muammer
Ekonomik krizlerin giderek daha sık yaşandığı ve bir ekonomiden diğerine hızlı bir biçimde yayılır hale geldiği günümüzde, krizlerin olumsuz etkilerinden asgari düzeyde etkilenmek için ülkelerin makroekonomilerinin sağlam temellere oturtulması büyük önem taşımaktadır. Ekonomik istikrarsızlığın büyüme, istihdam ve kalkınma gibi iktisadi hedefler üzerindeki olumsuz etkileri azımsanmayacak derecede yüksek olabilmektedir. Ekonomik büyüme ve kalkınma için yurtiçi kaynak sıkıntısı çeken, dolayısıyla dış kaynağa ihtiyaç duyan gelişmekte olan ülkeler için ekonomik ve siyasi istikrar önemli bir yer teşkil etmektedir. Dışsal değişkenlerin makroekonomik göstergeler üzerindeki etkilerinin ve makroekonomik göstergeler arasındaki ilişkileri iktisadi politika yapıcıların doğru teşhis edebilmeleri, söz konusu istikrarın sağlanmasına önemli katkıda bulunmaktadır. Bu çerçevede ithal ham petrol fiyatlarında meydana gelen değişimlerin makroekonomik aktiviteleri nasıl ve ne ölçüde etkilediği araştırılmak istenmiştir. Ayrıca, ithal ham petrol fiyatlarının fiyatlar genel düzeyindeki yeri ve önemi irdelenmeye çalışılmıştır. Bu olguyu Türkiye için ortaya koymak amacıyla, araştırmada 1986Q2-2010Q2 dönemini kapsayan üçer aylık veriler kullanılarak VAR yöntemiyle analiz yapılmıştır. Analiz sonucunda, ithal ham petrol fiyatlarının Türkiye’nin para politikası -özellikle para arzı- üzerinde daha fazla etkili olduğu tespit edilmiştir. Ayrıca, enflasyondaki değişimin kaynağının ne olduğu ile ilgili yapılan analizde ise özellikle ithal ham petrol fiyatları ve para politikasının fiyatlar genel düzeyindeki değişime kaynaklık ettiği görülmektedir. Teorik ve ampirik analiz sonucunda, ithal ham petrol fiyatları enflasyonun önemli bir kaynağı olduğu söylenebilir.
Citation - WoS: 10
Citation - Scopus: 10
Sonophotocatalytic Degradation Kinetics of an Azo Dye Amaranth
(Chem Soc Pakistan, 2012) Yetim, Tuba; Tekin, Taner
The effect of parameters such as initial concentration of dyestuff, light intensity, presence and the amplitude of ultrasound energy and temperature on sonophotocatalytic degradation kinetics of a monoazo dye amaranth (acid red 27-AR 27) was studied. The sonophotocatalytic degradation rate followed pseudo-first order kinetics with respect to amaranth concentrations. The ultrasound energy did not influence the activation energy. It was observed that the reaction rate accelerated in the presence of ultrasound energy during the experiments. A general equation was obtained for sonophotocatalytic degradation kinetics of amaranth which included the effect of ultrasound energy: (-dC(Dye)/dt)= 3,28 .10 (7) (1 + 0,0471 W (U) ) 0, 151 .exp( -7965 /T). I (alpha)(4,38/1 + 4,38 (C (D) ) (O))
Citation - WoS: 9
Citation - Scopus: 9
Effects of Lichenic Extracts (Hypogymnia Physodes, Ramalina Polymorpha and Usnea Florida) on Human Blood Cells: Cytogenetic and Biochemical Study
(Shaheed Beheshti Univ, Sch Pharmacy, 2012) Turkez, Hasan; Aydin, Elanur; Aslan, Ali
Several lichen species have been used for medicinal purposes throughout the ages, and they were reported to be effective in the treatment of different disorders including tuberculosis, hemorrhoids, ulcer, dysentery and cancer. It is revealed that they may be easily accessible sources of natural drugs that could be used as a possible food supplement or in pharmaceutical industry after their safety evaluations. However, so far, the nature and/or biological roles of plenty of lichenes have not been elucidated exactly. The aim of this study was to investigate the genetic and oxidative effects of water extracts of three different lichen species; Hypogymnia physodes, Ramalina polymorpha and Usnea florida in cultured human blood cells (n = 5) for the first time. All lichen species were collected from the Erzurum and Artvin provinces (in Turkey) during August 2010. The lichen extracts were added into culture tubes at various concentrations (0 to 2000 mg/L). Chromosome aberrations (CA) and micronucleus (MN) tests were used for genotoxic influences estimation. In addition, biochemical parameters (total antioxidant capacity (TAC) and total oxidative stress (TOS)) were examined to determine oxidative effects. In our in-vitro test systems, it was observed that all tested lichen extracts had no mutagenic effects on human lymphocytes. Furthermore, these extracts exhibited antioxidant properties due to the type of lichen species added to the cultures. In conclusion, these lichens can be a new resource of therapeutics as recognized in this study with their non-mutagenic and antioxidant features.
Citation - Scopus: 3
Experimantal and Theoretical Investigation of Fabricated Long Wavelength Tapered Semiconductor Lasers
(2012) Çakmak, B.B.; Duman, C.
In this study, fabrication and light output-current and longitudinal optical spectral characteristics of semiconductor lasers operating in the wavelengths of 1300 nm and 1550 nm are investigated. In addition, modelling of P-I and longitudinal optical spectrum characteristics of the lasers is obtained using rate equations. We observed that experimental results are well agreed with simulation. Threshold current is measured to be 30 mA while it is 27 mA in simulation for the lasers with 1300 nm and 1550 nm wavelengths. It is also found that the threshold current increases with increasing cavity length due to an increase in the losses. Furthermore, central wavelength of the optical spectra is obtained to be close to the design parameters. Finally, 3-dB spectral width is obtained experimentally and theoretically. 3-dB spectral width difference between experiment and simulation in 1550 nm lasers is obtained to be 1.6 nm while it is 10 nm in 1300 nm lasers. Because this difference obtained for 1550 nm lasers is much lower than that of 1330 nm lasers, it can be concluded that 1550 nm lasers have got better spectral stability than that of 1300 nm lasers.
Citation - WoS: 19
Citation - Scopus: 20
Modulatory Effects of Thymbra Spicata L. Different Extracts Against the Mercury Induced Genotoxicity in Human Lymphocytes in Vitro
(Springer, 2012) Dirican, Ebubekir; Turkez, Hasan; Togar, Basak
Mercury, a xenobiotic metal, is a highly deleterious environmental pollutant. Moreover, in any form mercury is reported to be toxic. On the other hand, Thymbra spicata L., a member of the Lamiaceae family, has long been investigated popularly of biological roles; mainly antimicrobial and antioxidant activities. However, there are very scarce data on the cytogenetic effects of thyme species. The purpose of this study was to investigate the genetic safety of different extracts from T. spicata (water extract, methanol extract, and ethanol extract) and the effects of T. spicata on mercury (as HgCl2) induced genotoxicity. Sister chromatid exchange (SCE) and micronucleus (MN) assays were performed to assess DNA damages in cultured human lymphocytes (n = 5). Our results clearly revealed that, the SCE and MN rates induced by HgCl2 were alleviated by the presence of T. spicata. As conclusion, this study demonstrated for the first time that the T. spicata provided increased resistance of DNA against HgCl2 induced genetic damage in human lymphocytes. Based on the results of this study, it may be concluded that the T. spicata is a nontoxic material that could be used as a suppressor of heavy metal-induced genotoxicity.
Citation - WoS: 30
Citation - Scopus: 30
In Vitro Studies on Chemoprotective Effect of Borax Against Aflatoxin B1-Induced Genetic Damage in Human Lymphocytes
(Springer, 2012) Turkez, Hasan; Geyikoglu, Fatime; Dirican, Ebubekir; Tatar, Abdulgani
A common dietary contaminant, aflatoxin B1 (AFB1), has been shown to be a potent mutagen and carcinogen in humans and many animal species. Since the eradication of AFB1 contamination in agricultural products has been rare, the use of natural or synthetic free radical scavengers could be a potential chemopreventive strategy. Boron compounds like borax (BX) and boric acid are the major components of industry and their antioxidant role has recently been reported. In the present report, we evaluated the capability of BX to inhibit the rate of micronucleus (MN) and sister chromatid exchange (SCE) formations induced by AFB1. There were significant increases (P < 0.05) in both SCE and MN frequencies of cultures treated with AFB1 (3.12 ppm) as compared to controls. However, co-application of BX (1, 2 and 5 ppm) and AFB1 resulted in decreases of SCE and MN rates as compared to the group treated with AFB1 alone. Borax gave 30-50 % protection against AFB1 induced SCEs and MNs. In conclusion, the support of borax was especially useful in aflatoxin-toxicated blood tissue. Thus, the risk on target tissues of AFB1 could be reduced and ensured early recovery from its toxicity.
Citation - WoS: 12
Citation - Scopus: 12
Propolis Protects Against 2,3,7,8-Tetrachlorodibenzo Toxicity in Rat Hepatocytes
(Pergamon-Elsevier Science Ltd, 2012) Turkez, Hasan; Yousef, Mokhtar I.; Geyikoglu, Fatime
The present experiment was undertaken to determine the effectiveness of propolis in alleviating the toxicity of TCDD on cultured primary rat hepatocytes. Propolis (25,50 and 100 mu M) was added to plain culture or simultaneously with TCDD (5 and 10 mu M). The hepatocytes were treated with TCDD and propolis for 48 h. Then cell viability was detected by [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MIT) assay and lactate dehydrogenase (LDH) release, while total antioxidant capacity (TAG) and total oxidative stress (TOS) levels were determined to evaluate the oxidative injury. The DNA damage was also analyzed by liver micronucleus assay (LMN) and 8-oxo-2-deoxyguanosine (8-OH-dG). The results of MTT and LDH assays showed that TCDD decreased cell viability. TCDD also increased TOS level and decreased TAC level in rat hepatocytes. On the basis of increasing doses, the TCDD caused significant increases of micronucleated hepatocytes (MNHEPs) and 8-OH-dG levels as compared to control culture. In cultures treated with propolis alone, cell viability and TOS level were not affected, while the level of TAG was significantly increased in dose-dependent fashion. The presence of propolis with TCDD modulated its toxic effects on primary hepatocytes cultures. Noteworthy, propolis has a protective effect against TCDD-mediated DNA damages. (C) 2011 Published by Elsevier Ltd.
Citation - WoS: 9
Citation - Scopus: 12
Molecular Characterization of Escherichia Coli O157:h7 from Retail Beef in Erzurum, Turkey
(Dr M N Khan, 2012) Adiguzel, G.; Gulluce, M.; Bozoglu, C.; Yanmis, D.; Gormez, A.; Atasever, M.; Adiguzel, A.
Ground meat is the leading fresh meat product for microbial contamination when considered by physical properties and preparation conditions. This study was conducted to determine the phenotypic and genotypic characterization of Escherichia coli 0157 strains isolated from one hundred and forty meat samples obtained from retail markets and butchers, Erzurum, Turkey. As a result %5 of meat samples are determined to contain target microorganism. Fatty acid profiles, metabolic fingerprints (BIOLOG), ERIC-PCR, REP-PCR, BOX-PCR and (GTG)(5)-PCR profiling methods were used for the phenotypic and genotypic characterization of O157:H7 isolates. The data of fatty acid analysis showed the presence of 20 different fatty acids in the 13 bacterial strains examined. All test strains were identified up to E. coli species level with Biolog system. After evaluating several primer sets targeting the repetitive DNA elements of REP, ERIC, BOX and (GTG)(5), the ERIC and (GTG)(5) primers were found to be the most reliable technique for identification and taxonomic characterization of E. coli 0157:H7 strains. Therefore, rep-PCR fingerprinting using the ERIC and (GTG)(5) primers can be considered as a promising genotypic tool for the identification and characterization of E. coli from species to serotype level.
Citation - WoS: 1
Thymbra Spicata L. Modulates Chromium (III) Chloride-Induced Genetic and Oxidative Damage in Vitro
(Parlar Scientific Publications (p S P), 2012) Dirican, Ebubekir; Turkez, Hasan
Chromium is a toxic heavy metal used in various industries and leads to environmental pollution due to improper handling. Cr exhibits oxidative stress mediated genotoxic damage although the mechanism of cellular damage caused by Cr has not been fully elucidated. On the other hand, Thymbra spicata L., a member of the Lamiaceae family, was recently investigated popularly of biological roles; mainly antioxidant and antimicrobial activities. However, there is very scarce data on the cytogenetic effects of Thyme species. Therefore, in this study, we aimed to determine whether T. spicata extracts conferred a protection against Chromium (III) chloride (CrCl3)-induced genotoxic and oxidative damage in vitro. For this aim, we determined sister chromatid exchange (SCE) rates and main antioxidant enzyme activities including catalase (CAT) superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in CrC3l (25 mu M) and T. spicata extracts (TSE) (25, 50, 100 mu M) treated human whole blood cultures (n=3) for 72h. T. spicata extracts at tested concentrations did not exhibit any negative effects on above studied parameters in culture tubes. Besides, the results of the present study indicated that the increases of SCE frequencies and the decreases of antioxidant enzyme activities by CrCl3 were minimized by the application of the T. spicata extracts. Our results firstly suggest that T. spicata augments the antioxidants defense against CrCl3 induced toxicity. Based on the results of this study, it may be concluded that dose controlled T. spicata diet may play a protective role in the process of Cr mutagenesis and/or carcinogenesis.
Citation - WoS: 6
Citation - Scopus: 5
Phenotyping and Genotyping Characterization of Salmonella Strains Isolated from Retail Beef in Erzurum, Turkey
(Dr M N Khan, 2012) Adiguzel, G.; Bozoglu, C.; Yanmis, D.; Gormez, A.; Gulluce, M.; Adiguzel, A.
In this study, phenotypic and genotypic characterization of Salmonella strains was carried out. Isolates were obtained from 140 different minced beef samples, taken from slaughterhouses and butcher shops in the vicinity of Erzurum, by using chromogenic media. For this purpose, fatty acid profiles, BIOLOG, ERIC-PCR, REP-PCR and (GTG)(5)-PCR methods were used. As a result of isolation study, Salmonella spp. was detected in 4 samples out of 140 minced beef samples. Depending on the data of fatty acid analysis, it was detected that there were 14 different fatty acids in the examined 15 bacterial strains. In the results of analyses by using BIOLOG system, all of the test organisms gave identification result as Salmonella group 1 and it was determined that this method is inefficient for serotype level detection. Several primer sets, targeting the repetitive DNA elements of REP, ERIC and (GTG)(5),were evaluated and it was detected that for identification and taxonomic characterization of S. typhimurium strains, the ERIC primers were the most reliable technique. Hence, as a promising genotypic tool, rep-PCR fingerprinting using the ERIC and REP primers can be used for the identification and characterization of Salmonella isolates species to serotype level.
Citation - WoS: 45
Citation - Scopus: 48
Efficient Production of L-Lactic Acid from Chicken Feather Protein Hydrolysate and Sugar Beet Molasses by the Newly Isolated Rhizopus Oryzae Ts-61
(Elsevier, 2012) Taskin, Mesut; Esim, Nevzat; Ortucu, Serkan
The aim of this study was to investigate production of L-lactic acid from molasses and chicken feather protein hydrolysate (CFP) by the newly isolated Rhizopus oryzae TS-61. R. oryzae TS-61 was capable of utilizing molasses sucrose and CFP as carbon and nitrogen sources, respectively. In contrast to yeast extract and ammonium sulfate, CFP had potential not only to prevent excessive pH changes and foaming but also to provide smaller uniform pellet formation in during fermentation. Thanks to these properties, it was concluded that CFP might have resulted in higher L-lactic acid production than the other two nitrogen sources (yeast extract and ammonium sulfate). At the end of 42-h optimal cultivation period, the highest (38.5 g/L) and lowest (28.8 g/L) concentrations of L-lactic acid were obtained with CFP and ammonium sulfate, respectively. This is the first report on use of waste chicken feather as a lactic acid production substrate. In addition, a new R. oryzae strain, being capable of using molasses sucrose as carbon source in order to produce L-lactic acid, was isolated. (C) 2012 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

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