Scopus İndeksli Yayınlar Koleksiyonu

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Citation - WoS: 137
Citation - Scopus: 144
Acetylcholinesterase and Carbonic Anhydrase Inhibitory Properties of Novel Urea and Sulfamide Derivatives Incorporating Dopaminergic 2-Aminotetralin Scaffolds
(Pergamon-Elsevier Science Ltd, 2016) Ozgeris, Bunyamin; Goksu, Suleyman; Kose, Leyla Polat; Gulcin, Ilhami; Salmas, Ramin Ekhteiari; Durdagi, Serdar; Supuran, Claudiu T.
In the present study a series of urea and sulfamide compounds incorporating the tetralin scaffolds were synthesized and evaluated for their acetylcholinesterase (AChE), human carbonic anhydrase (CA, EC 4.2.1.1) isoenzyme I, and II (hCA I and hCA II) inhibitory properties. The urea and their sulfamide analogs were synthesized from the reactions of 2-aminotetralins with N,N-dimethylcarbamoyl chloride and N,N-dimethylsulfamoyl chloride, followed by conversion to the corresponding phenols via O-demethylation with BBr3. The novel urea and sulfamide derivatives were tested for inhibition of hCA I, II and AChE enzymes. These derivatives exhibited excellent inhibitory effects, in the low nanomolar range, with K-i values of 2.61-3.69 nM against hCA I, 1.64-2.80 nM against hCA II, and in the range of 0.45-1.74 nM against AChE. In silico techniques such as, atomistic molecular dynamics (MD) and molecular docking simulations, were used to understand the scenario of the inhibition mechanism upon approaching of the ligands into the active site of the target enzymes. In light of the experimental and computational results, crucial amino acids playing a role in the stabilization of the enzyme-inhibitor adducts were identified. (C) 2016 Elsevier Ltd. All rights reserved.
Citation - WoS: 31
Citation - Scopus: 32
Acute and Chronic Effects of Aerobic Exercise on Serum Irisin, Adropin, and Cholesterol Levels in the Winter Season: Indoor Training Versus Outdoor Training
(Wolters Kluwer Medknow Publications, 2020) Ozbay, Serhat; Ulupinar, Suleyman; Sebin, Engin; Altinkaynak, Konca
The aim of this study is to investigate the acute and chronic effects of aerobic training performed indoors and outdoors on irisin, adropin, and cholesterol levels in winter. Thirty-two healthy males participated in this study. Participants were divided into two groups: outdoor group (n = 16) and indoor group (n = 16). They then performed 40-min aerobic running exercises 4 days/week for 18 weeks. The outdoor group trained at -5 degrees C-5 degrees C environmental temperature, while the indoor group trained at 21 degrees C-25 degrees C. Blood samples were collected before and after the 18-week training period and immediately after the first training. The results showed that single aerobic exercise induced minimal increase in serum irisin concentrations in both groups. In addition, irisin levels did not change in the outdoor group but significantly decreased in the indoor group after the 18-week training period. Aerobic exercise had no acute or chronic effects on serum adropin levels in the indoor group. However, the aerobic training caused a decrease in adropin levels chronically, but there was no acute effect after single aerobic exercise in the outdoor group. Furthermore, there was no acute effect on high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol, and total cholesterol after single aerobic exercise in both groups. However, after the 18-week training period, there was a significant increase in HDL-C levels in both groups. Moreover, the increase in HDL-C in the outdoor group was higher than in the indoor group. Thus, this study provides evidence for the beneficial chronic effects of aerobic exercise and cold on HDL-C levels as well as the beneficial acute effects on irisin concentrations.
Citation - WoS: 7
Citation - Scopus: 7
The Acute Effects of Hip Thrust and Glute Bridge Exercises with Different Loads on Sprint Performance and Horizontal Force-Velocity Profile in Adolescent Soccer Players: A Post-Activation Performance Enhancement Approach
(Wiley, 2025) Cabuk, Salih; Ince, Izzet
This study examines the acute effects of post-activation performance enhancement (PAPE) with different loads (84% and 60% 1-RM) and exercises (hip thrust; HT and glute bridge; GB) on sprint performance (SP) and horizontal force-velocity (HF-V) profile components in adolescent male soccer players. The participants were randomly assigned to three groups: hip thrust group (HTG; n = 13), glute bridge group (GBG; n = 13), and control group (CG; n = 14). Sprint tests at distances of 10, 20, and 30 m were conducted pre-PAPE and post-PAPE protocols with a 7 min rest period. HTG and GBG executed HT and GB exercises at 84% and 60% of their 1-RM loads. Hedge's g was computed to assess within-group (pre-PAPE vs. post-PAPE) comparisons (Within-ES) and between-group (post-PAPE protocols) comparisons (Between-ES). PAPE protocols at 84% and 60% 1-RM loads demonstrated moderate effects on F0rel, Pmaxrel, FVslope, and DRF and small effects on V0, RFmax, and S20m in both HTG and GBG. Conversely, the CG exhibited trivial effects across parameters. Compared to the HTG 84% 1-RM protocol, the GBG 84% 1-RM protocol showed small effects on V0 and S10m. The HTG 60% 1-RM protocol had a small effect on RFmax compared to both GBG 84% and 60% 1-RM protocols. Both HTG and GBG 84% and 60% 1-RM protocols demonstrated small effects on S30m compared to the CG. These findings suggest that GB exercises may offer a viable alternative to HT exercises for eliciting PAPE effects, particularly in enhancing SP and related mechanics in adolescent soccer players.
Citation - WoS: 1
Citation - Scopus: 1
Acute Effects of Nordic Hamstring Exercise on Hamstring Stiffness: A Randomised Controlled Trial
(MDPI, 2025) Yagiz, Gokhan; Monleon, Cristina; Akaras, Esedullah; Adanir, Sena; Liebana, Encarnacion
Background: This study aimed to examine the immediate effects of the Nordic Hamstring Exercise (NHE) on the stiffness of the biceps femoris (BF) and semitendinosus (ST) muscles. Methods: This parallel-group randomised controlled trial followed CONSORT 2025 guidelines. Twenty-four physically active adults (16 females, 8 males) were randomly assigned to an NHE group (n = 12) or a control group (n = 12) using 1:1 gender-stratified randomisation. The NHE group performed 3 sets of 10 repetitions of the NHE, while the control group remained inactive. Muscle stiffness of the BF and ST was assessed pre- and post-intervention using the MyotonPro device. Results: No significant overall changes in hamstring stiffness were observed within or between groups (p > 0.05). Within the NHE group, the ST tended to increase in stiffness (11.25 N/m, p = 0.057), while the BF showed a small, non-significant reduction (-12.00 N/m, p = 0.696). The difference in changes between BF and ST was significant (p = 0.039). Independent of group allocation, males demonstrated significantly higher baseline stiffness than females for BF (258.13 vs. 195.81 N/m, p < 0.001) and for ST (247.88 vs. 174.00 N/m, p = 0.003). Regression analysis showed that only height predicted the change in ST stiffness after NHE (R = 0.625, R-2 = 0.39, p = 0.030). Conclusions: A single NHE session did not alter overall hamstring stiffness but produced opposite, muscle-specific effects. More research with larger, uniform samples is needed to confirm these findings.
Adherence to the Mediterranean Diet in Adults: The Role of Environmental and Sustainable Food Literacy
(BMC, 2025) Ozata-Uyar, Gizem; Aslan, Serkan; Camli, Ayse; Arslan, Merve; Bozkurt, Osman; Kocaadam-Bozkurt, Betul
AimThis study aimed to evaluate the relationship between environmental literacy, sustainable food literacy, and adherence to the Mediterranean diet (MD) in adults.MethodData were collected via a web-based questionnaire created using Google Forms between April and August 2024 in Erzurum, Turkey. A total of 394 adults participated through snowball sampling. The questionnaire included sections on demographic information, anthropometric measurements, the Environmental Literacy Scale for Adults (ELSA), the Sustainable Food Literacy Scale (SFLS), and the Mediterranean Diet Adherence Screener (MEDAS).Results38.6% of the participants were found to adhere to the Mediterranean Diet (MD). Married participants were more likely to have adhered to the MD than single ones (odds ratio [OR]:0.41, p = 0.006). Adherence to the MD was associated with higher education duration (OR = 1.05, p = 0.011), a higher SFLS total score (OR = 1.01, p = 0.025), and a higher ELSA score (OR = 1.04, p = 0.004).ConclusionThe MD is a sustainable dietary model that enhances health and is environmentally friendly. For this reason, it may be possible to direct society towards healthier and less environmentally damaging food choices through training to increase individuals' sustainable literacy and environmental literacy levels.
Citation - WoS: 1
Citation - Scopus: 1
Advancing Pulmonary Embolism Detection with Integrated Deep Learning Architectures
(Springer, 2025) Biret, Can Berk; Gurbuz, Sukru; Akbal, Erhan; Baygin, Mehmet; Ekingen, Evren; Derya, Serdar; Tuncer, Turker
The main aim of this study is to introduce a new hybrid deep learning model for biomedical image classification. We propose a novel convolutional neural network (CNN), named HybridNeXt, for detecting pulmonary embolism (PE) from computed tomography (CT) images. To evaluate the HybridNeXt model, we created a new dataset consisting of two classes: (1) PE and (2) control. The HybridNeXt architecture combines different advanced CNN blocks, including MobileNet, ResNet, ConvNeXt, and Swin Transformer. We specifically designed this model to combine the strengths of these well-known CNNs. The architecture also includes stem, downsampling, and output stages. By adjusting the parameters, we developed a lightweight version of HybridNeXt, suitable for clinical use. To further improve the classification performance and demonstrate transfer learning capability, we proposed a deep feature engineering (DFE) method using a multilevel discrete wavelet transform (MDWT). This DFE model has three main phases: (i) feature extraction from raw images and wavelet bands, (ii) feature selection using iterative neighborhood component analysis (INCA), and (iii) classification using a k-nearest neighbors (kNN) classifier. We first trained HybridNeXt on the training images, creating a pretrained HybridNeXt model. Then, using this pretrained model, we extracted features and applied the proposed DFE method for classification. The HybridNeXt model achieved a test accuracy of 90.14%, while our DFE model improved accuracy to 96.35%. Overall, the results confirm that our HybridNeXt architecture is highly accurate and effective for biomedical image classification. The presented HybridNeXt and HybridNeXt-based DFE methods can potentially be applied to other image classification tasks.
Citation - WoS: 62
Citation - Scopus: 70
The Advent of Indium Selenide: Synthesis, Electronic Properties, Ambient Stability and Applications
(MDPI AG, 2017) Boukhvalov, Danil W.; Gurbulak, Bekir; Duman, Songul; Wang, Lin; Politano, Antonio; Caputi, Lorenzo S.; Cupolillo, Anna
Among the various two-dimensional semiconductors, indium selenide has recently triggered the interest of scientific community, due to its band gap matching the visible region of the electromagnetic spectrum, with subsequent potential applications in optoelectronics and especially in photodetection. In this feature article, we discuss the main issues in the synthesis, the ambient stability and the application capabilities of this novel class of two-dimensional semiconductors, by evidencing open challenges and pitfalls. In particular, we evidence how the growth of single crystals with reduced amount of Se vacancies is crucial in the road map for the exploitation of indium selenide in technology through ambient-stable nanodevices with outstanding values of both mobility of charge carriers and ON/OFF ratio. The surface chemical reactivity of the InSe surface, as well as applications in the fields of broadband photodetection, flexible electronics and solar energy conversion are also discussed.
Citation - Scopus: 1
Aerobic Capacity and Respiratory Indices of Junior Cross-Country Skiers and Biathletes during Incremental Exercise Testing
(Nature Portfolio, 2024) Tortu, Erkan; Ouergui, Ibrahim; Deliceoglu, Gokhan; Keles, Ali; Ulupinar, Suleyman; Ozbay, Serhat; Ardigo, Luca Paolo
The present study compared the isocapnic buffering phase (ICB), hypocapnic hyperventilation phase, ventilatory threshold (VT), respiratory compensation point (RCP), and maximum oxygen uptake (VO2max) among biathlon and cross-country ski athletes during an incremental exercise test. 37 male and 33 female Turkish National Team athletes volunteered to participate in the research. Body fat percentage, lean mass, and fat mass values of athletes were measured using the bioelectrical impedance analysis method, and oxygen consumption (VO2) was measured with a portable cardiopulmonary exercise test system with a ramp protocol on the treadmill. In VT, RCP, and VO2max phases, male athletes had higher VO2 and speed values than female athletes (p 0.05). Additionally, XC skiers and males had higher absolute (Abs) VO2 and mass-normalized (Rel) VO2 values than biathletes and females in exhaustion times and ICBs (p 0.05). In addition, XC skiers and males had higher absolute (Abs) VO(2 )and relative (Rel) VO2 values than biathletes and females in exhaustion times and ICBs (p 0.05). These results indicate significant differences in physiological profiles between male and female athletes and between XC skiers and biathletes.
Citation - WoS: 41
Citation - Scopus: 43
Aerobic Exercise Increases Irisin Serum Levels and Improves Depression and Fatigue in Patients with Relapsing Remitting Multiple Sclerosis: A Randomized Controlled Trial
(Elsevier Sci Ltd, 2022) Bilek, Furkan; Cetisli-Korkmaz, Nilufer; Ercan, Zubeyde; Deniz, Gulnihal; Demir, Caner Feyzi
Background: Multiple sclerosis (MS) is a chronic progressive neurodegenerative disease of the central nervous system. Although there is increasing evidence that aerobic exercise has a positive effect on both cognitive and psychological functioning, there is limited evidence for Relapsing-Remitting MS (RRMS) patients. Moreover, it is unclear at what exercise modality and intensity the irisin, the cleaved and circulating form of the exerciseassociated membrane protein Fibronectin Type III Domain Containing 5, is induced in patients with MS. This study aimed to investigate the effect of a regular aerobic exercise program on irisin serum level, depression, fatigue, and cognitive performance in patients with MS. Methods: Thirty-two individuals with RRMS were randomized into 2 groups as control and study groups (mean EDSS score 1.69 and 1.97, respectively). While the Study Group received a combined exercise training consisting of three sessions of aerobic exercise and Frenkel Coordination Exercises per week for 6 weeks, the Control Group received only Frenkel Coordination Exercise training. Before and after the study, the cognitive performance of the participants were evaluated with the Paced Auditory Serial Addition Test with 3-second stimulus (PASAT-3), their maximum aerobic capacity with the Fitmate Pro (R) (VO2max), their fatigue status with the Fatigue Impact Scale (FIS), and their depression status with the Beck Depression Inventory (BDI). Irisin serum levels were analyzed with Enzyme-Linked ImmunoSorbent Assay (ELISA) test from the serum samples of individuals. Results: Our results revealed that the irisin serum level significantly increased in the Study Group. Significant improvement in aerobic capacity, PASAT-3, FIS, and BDI values was observed in the Study Group compared to the Control Group. When the Delta Irisin, Delta VO2max, Delta FIS, and Delta BDI values between the groups were compared, a significant difference was found in favor of the study group. Conclusion: The aerobic exercise revealed significant changes in depression, fatigue and irisin serum levels in MS patients. We think that this study, in which a significant increase in irisin serum level, significant improvement in depression, cognitive performance and fatigue states were obtained in the Study Group, will be a pioneering study for the future studies aiming to investigate the effects of irisin serum level on these symptoms in detail.
Citation - WoS: 11
Citation - Scopus: 13
Affinity-Based and In a Single Step Purification of Recombinant Horseradish Peroxidase A2a Isoenzyme Produced by Pichia Pastoris
(Springer, 2023) Acar, Melek; Abul, Nurgul; Yildiz, Seyda; Taskesenligil, Ezgi Dag; Gerni, Serpil; Unver, Yagmur; Ozdemir, Hasan
Horseradish peroxidase (HRP) is an oxidoreductase enzyme and oxidizes various inorganic and organic compounds. It has wide application areas such as immunological tests, probe-based test techniques, removal of phenolic pollutants from wastewater and organic synthesis. HRP is found in the root of the horseradish plant as a mixture of different isoenzymes, and it is very difficult to separate these enzymes from each other. In this regard, recombinant production is a very advantageous method in terms of producing the desired isoenzyme. This study was performed to produce HRP A2A isoenzyme extracellularly in Pichia pastoris and to purify this enzyme in a single step using a 3-amino-4-chloro benzohydrazide affinity column. First, codon-optimized HRP A2A gene was amplified and inserted into pPICZ alpha C. So, obtained pPICZ alpha C-HRPA2A was cloned in E. coli cells. Then, P. pastoris X-33 cells were transformed with linearized recombinant DNA and a yeast clone was cultivated for extracellular recombinant HRP A2A (rHRP A2A) enzyme production. Then, the purification of this enzyme was performed in a single step by affinity chromatography. The molecular mass of purified rHRP A2A enzyme was found to be about 40 kDa. According to characterization studies of the purified enzyme, the optimum pH and ionic strength for the rHRP A2A isoenzyme were determined to be 6.0 and 0.04 M, respectively, and o-dianisidine had the highest specificity with the lowest Km and Vmax values. Thus, this is an economical procedure to purify HRP A2A isoenzyme without time-consuming and laborious isolation from an isoenzyme mixture.
Citation - WoS: 5
Citation - Scopus: 3
Alleviation of LPS-Induced Acute Lung Injury by Propolis-Based Nanocomposites Through the TLR4/NFκB and P2X7/Akt Pathways: Randomized-Controlled Experimental Study
(Pergamon-Elsevier Science Ltd, 2025) Ustundag, Hilal; Kara, Adem; Tas, Necip Gokhan; Kalindemirtas, Ferdane Danisman; Kurt, Nezahat; Erbas, Elif; Kariper, Ishak Afsin
Sepsis-associated acute lung injury continues to pose a significant medical challenge with substantial morbidity and mortality rates. In this study, we investigated the therapeutic potential of propolis-based treatments and their nanocomposites in modulating inflammation and apoptosis using a lipopolysaccharide (LPS)-induced rat model of sepsis. Forty-two Sprague-Dawley rats were divided into seven groups (n = 6): control, LPS (5 mg/kg, i. p.), LPS + Propolis (100 mg/kg, i.p.), LPS + NanoPropolis (100 mg/kg, i.p.), LPS + silver nanoparticles propolis (AgNPsPro) (50 mg/kg), and a negative propolis group (100 mg/kg, i.p.). The rats were assessed for inflammatory, oxidative stress, and apoptotic markers through Western blot, histopathological analyses, and biochemical measurements. The LPS group exhibited significantly higher levels of pro-inflammatory cytokines (IL-1 beta, TNF-alpha) and the systemic infection marker presepsin (PRSN) in blood, as well as the oxidative stress marker malondialdehyde (MDA) in lung tissue. The treatment groups, particularly LPS + AgNPsPro, showed significant reductions in these markers, with decreased levels of MDA, IL-1 beta, TNF-alpha, NF-kappa B, and TLR4, and increased GSH content in lung tissue (p < 0.05). The anti-apoptotic protein BCL-2 was upregulated, while proapoptotic BAX expression was reduced, indicating enhanced cell survival. The P2X7 receptor, a key inflammation regulator, and the AKT signaling pathway, involved in cell survival, were positively modulated by the treatments. Histopathological findings corroborated these results, showing less lung tissue damage. In conclusion, propolisbased treatments, especially in combination with nanoparticles, demonstrate therapeutic potential in reducing inflammation, oxidative stress, and apoptosis in sepsis-induced lung injury.
Alleviation of LPS-Induced Acute Lung Injury by Propolis-Based Nanocomposites Through the TLR4/NFκB and P2X7/Akt Pathways: Randomized-Controlled Experimental Study (Vol 258, 108330, 2025)
(Pergamon-Elsevier Science Ltd, 2025) Ustundag, Hilal; Kara, Adem; Tas, Necip Gokhan; Kalindemirtas, Ferdane Danisman; Kurt, Nezahat; Erbas, Elif; Kariper, Ishakshak Afsin
Citation - WoS: 21
Citation - Scopus: 19
Alpha-B Expression in Human Laryngeal Squamous Cell Carcinoma Tissues
(Wiley, 2015) Yilmaz, Mehmet; Karatas, Omer Faruk; Yuceturk, Betul; Dag, Huseyin; Yener, Murat; Ozen, Mustafa
BackgroundLaryngeal squamous cell carcinoma (SCC), being an aggressive malignancy, is one of the most commonly diagnosed malignant types of head and neck SCC worldwide. The recent studies suggested that B-crystallin might play an important role in tumorigenesis. The purpose of this study was to investigate the B-crystallin expression level in metastatic and nonmetastatic laryngeal SCC tissues and to determine its prognostic significance. MethodsAlpha-B-crystallin expression status in metastatic, nonmetastatic laryngeal SCC, and normal tissue samples was investigated by quantitative real-time polymerase chain reaction (qRT-PCR). ResultsWe demonstrated that the expression of B-crystallin was significantly upregulated in laryngeal SCC tumor tissue samples in comparison with the corresponding normal tissues (p<.001), although no significant association has been found between B-crystallin expression and either the metastatic potential or the T classification of the specimens. ConclusionAlthough expression of B-crystallin is not statistically correlated with neck metastases, we demonstrated that B-crystallin is significantly overexpressed in laryngeal cancer. (c) 2014 Wiley Periodicals, Inc. Head Neck 37: 1344-1348, 2015
Citation - WoS: 41
Citation - Scopus: 47
The Altered Promoter Methylation of Oxytocin Receptor Gene in Autism
(Taylor & Francis Ltd, 2016) Yuksel, Mine Elagoz; Yuceturk, Betul; Karatas, Omer Faruk; Ozen, Mustafa; Dogangung, Burak
Autism spectrum disorder (ASD) is one of the lifelong existing disorders. Abnormal methylation status of gene promoters of oxytonergic system has been implicated as among the etiologic factors of ASDs. We, therefore, investigated the methylation frequency of oxytocin receptor gene (OXTR) promoter from peripheral blood samples of children with autistic features. Our sample includes 66 children in total (22-94 months); 27 children with ASDs according to the DSM-IV-TR and the Childhood Autism Rating Scale (CARS) and 39 children who do not have any autistic like symptoms as the healthy control group. We investigated the DNA methylation status of OXTR promoter by methylation specific enzymatic digestion of genomic DNA and polymerase chain reaction. A significant relationship has been found between ASDs and healthy controls for the reduction of methylation frequency of the regions MT1 and MT3 of OXTR. We could not find any association in the methylation frequency of MT2 and MT4 regions of OXTR. Although our findings indicate high frequency of OXTR promoter hypomethylation in ASDs, there is need for independent replication of the results for a bigger sample set. We expect that future studies with the inclusion of larger, more homogeneous samples will attempt to disentangle the causes of ASDs.
Citation - WoS: 4
Citation - Scopus: 7
Aluminium Phosphide-Induced Genetic and Oxidative Damages in Vitro: Attenuation by Laurus Nobilis L. Leaf Extract
(Wolters Kluwer Medknow Publications, 2013) Turkez, Hasan; Togar, Basak
Objective: The present investigation was undertaken to assess the protective effect of Laurus nobilis leaf extract (LNE) against aluminum phosphide (AIP)-induced genotoxic and oxidative damages stress in cultured human blood cells in the presence of a metabolic activator (S9 mix). Materials and Methods: Sister chromatid exchange (SCE) and chromosome aberration (CA) assays were used to assess AlP-induced genotoxicity and to establish the protective effects of LNE. In addition, we determined total antioxidant capacity (TAC) and total oxidative status (TOS) levels in AlP and LNE treated cultures for biomonitoring the oxidative alterations. Results: There was significant increases (P < 0.05) in both SCE and CA frequencies of cultures treated with AlP as compared to controls. Our results also showed that AlP (58 mg/l) caused oxidative stress by altering TAC and TOS levels. However, co-application of LNE (25, 50, 100 and 200 mg/l) and AlP resulted in decreases of SCE, CA rates and TOS level and increases of TAC level as compared to the group treated with AlP alone. Conclusion: The preventive role of LNE in alleviating AlP-induced DNA and oxidative damages was indicated for the first time in the present study.
Citation - WoS: 15
Citation - Scopus: 16
Aluminum Phosphide-Induced Genetic and Oxidative Damages in Rats: Attenuation by Laurus Nobilis Leaf Extract
(Sage Publications Inc, 2013) Turkez, Hasan; Togar, Basak
Aluminum phosphide (AlP) is a colorless, flammable, liquefied pesticide that is commonly used to control insects, nematodes, weeds, and pathogens in crops, forests, ornamental nurseries, and wood products. Early investigations of AlP-poisoned mammalian cells led to the proposed involvement of oxidative damage in its toxicity mechanism. Therefore, this study was aimed to evaluate the effect of Laurus nobilis (L) leaf extract (LNE) against AlP-induced genetic and oxidative damages in rats. Selected animals were assigned to four groups (n = 6), namely, group A: control (only distilled water is injected); group B: AlP (4 mg kg(-1) injected intraperitoneally (i.p.)); group C: LNE (200 mg kg(-1) injected i.p.), and group D: AlP plus LNE, respectively. The experimental period lasted for 14 successive days. Chromosomal aberrations (CAs) and micronucleus (MN) assay were used for monitoring genotoxic damage. In addition, biochemical parameters such as total antioxidant capacity (TAC) and total oxidative status (TOS) were examined in serum samples to determine oxidative damage. Our results indicated that AlP caused increase in CA and MN assay rates and alterations in TAC and TOS levels when compared with control group. On the contrary, LNE did not change the rates of both the analyzed cytogenetic end points and led to increase in TAC level. Moreover, we observed that LNE suppressed the genetic damage by AlP to bone marrow cells in vivo. Interestingly AlP-induced oxidative stress was also strongly reduced by LNE. The results of the present study indicated that the protective effect of LNE might be ascribable to its antioxidant and free radical scavenging properties.
Citation - WoS: 7
Citation - Scopus: 10
The Ameliorative Effect of Cetraria Islandica Against Diabetes-Induced Genetic and Oxidative Damage in Human Blood
(Taylor & Francis Ltd, 2013) Colak, Suat; Geyikoglu, Fatime; Turkez, Hasan; Bakir, Tulay Ozhan; Aslan, Ali
Context: The aqueous extracts of Cetraria islandica (L.) Ach. (Parmeliaceae) is traditionally used in many countries against a number of conditions, including inflammatory conditions. Objective: The present study aimed to assess, for the first time, the effectiveness of C. islandica in cultured primary blood cells of Type 1 diabetes subjects. Materials and methods: Diabetic and control blood samples were treated with or without aqueous lichen extract (5 and 10 mu g mL(-1)) for 48 h. The activity of antioxidant enzymes in erythrocytes and also malondialdehyde levels in plasma were determined to evaluate the oxidative status. DNA damages were analyzed by SCE, MN and comet assays in cultured human lymphocytes. Additionally, proliferation index (PI) was evaluated in peripheral blood lymphocytes. Results: There were significant increases in observed total DNA damage (comet assay) (240.2%) and SCE (168.8%), but not in MN frequencies of cultures with diabetes as compared (p>0.05) to controls. Whereas, the significant reductions of total DNA damage (69.2 and 65.3%) and SCE frequencies (17.7 and 12.3%) were determined when the 5 and 10 mg mL(-1) lichen extract was added to the cell culture medium, respectively. However, lichen extract did not completely inhibit the induction of SCEs in lymphocytes of patients with diabetes. C. islandica extract was also useful on PI rates. Discussion: In conclusion, the antioxidant role of C. islandica in alleviating diabetes-induced genomic instability and for increasing cell viability was firstly indicated in the present study.
Citation - WoS: 22
Citation - Scopus: 22
Ameliorative Effect of Supplementation with L-Glutamine on Oxidative Stress, DNA Damage, Cell Viability and Hepatotoxicity Induced by 2,3,7,8-Tetrachlorodibenzo in Rat Hepatocyte Cultures
(Springer, 2012) Turkez, Hasan; Geyikoglu, Fatime; Yousef, Mokhtar I.; Celik, Kubra; Bakir, Tulay O.
The most potent of the dioxins, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is a persistent and ubiquitous environmental contaminant. And the health impact of exposure to TCDD is of great concern to the general public. Recent data indicate that l-glutamine (Gln) has antioxidant properties and may influence hepatotoxicity. The objective of the present study was undertaken to explore the effectiveness of Gln in alleviating the hepatotoxicity of TCDD on primary cultured rat hepatocytes. Gln (0.5, 1 and 2 mM) was added to cultures alone or simultaneously with TCDD (0.005 and 0.01 mM). The hepatocytes were treated with TCDD and Gln for 48 h. Then cell viability was detected by [3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide] (MTT) assay and lactate dehydrogenase (LDH) release, while total antioxidant capacity (TAC), total glutathione (TGSH) and total oxidative stress (TOS) levels were determined to evaluate the oxidative injury. The DNA damage was also analyzed by liver micronucleus assay (MN) and 8-oxo-2-deoxyguanosine (8-OH-dG). The results of MTT and LDH assays showed that TCDD decreased cell viability but not l-glutamine. TCDD also increased TOS level in rat hepatocytes and significantly decreased TAC and TGSH levels. On the basis of increasing doses, the dioxin in a dose-dependent manner caused significant increases of micronucleated hepatocytes (MNHEPs) and 8-OH-dG as compared to control culture. Whereas, in cultures exposured with Gln alone, TOS levels were not changed and TAC and TGSH together were significantly increased in dose-dependent fashion. The presence of Gln with TCDD modulated the hepatotoxic effects of TCDD on primary hepatocytes cultures. Noteworthy, Gln has a protective effect against TCDD-mediated DNA damages. As conclusion, we reported here an increased potential therapeutic significance of l-glutamine in TCDD-mediated hepatic injury for the first time.
Citation - WoS: 12
Citation - Scopus: 14
Ameliorative Effects by Hexagonal Boron Nitride Nanoparticles Against Beta Amyloid Induced Neurotoxicity
(MDPI, 2022) Aydin, Nursah; Turkez, Hasan; Tozlu, Ozlem Ozdemir; Arslan, Mehmet Enes; Yavuz, Mehmet; Sonmez, Erdal; Mardinoglu, Adil
Alzheimer's disease (AD) is considered as the most common neurodegenerative disease. Extracellular amyloid beta (A beta) deposition is a hallmark of AD. The options based on degradation and clearance of A beta are preferred as promising therapeutic strategies for AD. Interestingly, recent findings indicate that boron nanoparticles not only act as a carrier but also play key roles in mediating biological effects. In the present study, the aim was to investigate the effects of different concentrations (0-500 mg/L) of hexagonal boron nitride nanoparticles (hBN-NPs) against neurotoxicity by beta amyloid (A beta(1-42)) in differentiated human SH-SY5Y neuroblastoma cell cultures for the first time. The synthesized hBN-NPs were characterized by X-ray diffraction (XRD) measurements, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). A beta(1-42)-induced neurotoxicity and therapeutic potential by hBN-NPs were assessed on differentiated SH-SY5Y cells using MTT and LDH release assays. Levels of total antioxidant capacity (TAC) and total oxidant status (TOS), expression levels of genes associated with AD and cellular morphologies were examined. The exposure to A beta(1-42) significantly decreased the rates of viable cells which was accompanied by elevated TOS level. A beta(1-42) induced both apoptotic and necrotic cell death. A beta exposure led to significant increases in expression levels of APOE, BACE 1, EGFR, NCTSN and TNF-alpha genes and significant decreases in expression levels of ADAM 10, APH1A, BDNF, PSEN1 and PSENEN genes (p < 0.05). All the A beta(1-42)-induced neurotoxic insults were inhibited by the applications with hBN-NPs. hBN-NPs also suppressed the remarkable elevation in the signal for A beta following exposure to A beta(1-42) for 48 h. Our results indicated that hBN-NPs could significantly prevent the neurotoxic damages by A beta. Thus, hBN-NPs could be a novel and promising anti-AD agent for effective drug development, bio-nano imaging or drug delivery strategies.
Citation - WoS: 8
Citation - Scopus: 10
Ameliorative Effects of Docosahexaenoic Acid on the Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo in Cultured Rat Hepatocytes
(Sage Publications Inc, 2016) Turkez, Hasan; Geyikoglu, Fatime; Yousef, Mokhtar I.
The 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is an environmental contaminant toxicant that mediates carcinogenic effects associated with oxidative DNA damage. Docosahexaenoic acid (DHA) with antioxidant functions has many biochemical, cellular, and physiological functions for cells. The present study assessed, for the first time, the ameliorative effect of DHA in alleviating the toxicity of TCDD on primary cultured rat hepatocytes (HEPs). In vitro, isolated HEPs were incubated with TCDD (5 and 10 M) in the presence and absence of DHA (5, 10, and 20 M) for 48 h. The cell viability was detected by 3-(4,5-dimethylthiazol-2-yl) 2,5diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) release. DNA damage was analyzed by liver micronucleus assay and 8-oxo-2-deoxyguanosine (8-OH-dG) level. In addition, total antioxidant capacity (TAC) and total oxidative stress (TOS) were assessed to determine the oxidative injury in HEPs. The results of MTT and LDH assays showed that TCDD decreased cell viability but not DHA. On the basis of increasing treatment concentrations, the dioxin caused significant increases of micronucleated HEPs and 8-OH-dG as compared to control culture. TCDD also led to significant increases in TOS content. On the contrary, in cultures treated with DHA, the level of TAC was significantly increased during treatment in a concentration-dependent fashion. DHA showed therapeutic potential against TCDD-mediated cell viability and DNA damages. As conclusion, this study provides the first evidence that DHA has protective effects against TCDD toxicity on primary cultured rat hepatocytes.

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