Scopus İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14901/696

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Citation - WoS: 3
Citation - Scopus: 4
Acute Effects of Three Different Stretching Techniques on Hamstring Flexibility in Professional Football Players
(Gdansk Univ Physical Education & Sport, 2023) Erol, Erkan; Yildiz, Ramazan; Yildiz, Aye; Dogan, Fatih Emre; Elbasan, Buelent
Introduction: Hamstring injuries are common in physically active people and athletes. Lack of hamstring flexibility is the one of the most important characteristics of hamstring injuries in athletes. The aim of this study was to investigate effects of three different stretching techniques on hamstring flexibility in professional football players. Materials and Methods: Fifty-five male football players between the ages of 18-20 years old were involved in the study. The participants were divided into 3 groups: static stretching, neuromobilization and Mulligan traction straight leg raise (TSLR), respectively. Each participant's dominant lower extremity was assessed with a straight leg raise test (SLR) using a goniometer before and after the intervention. Results: There was a significant difference in the SLR range of motion before and after the intervention in all groups. There was no difference in the range of SLR between the groups at the beginning and at the end. However, the increase in the SLR range of motion was significantly higher in the Mulligan TSLR group. Conclusions: It was concluded that all 3 stretching techniques are effective in increasing hamstring flexibility in football players. However, the Mulligan TSLR technique could provide a greater increase in the range of motion than other techniques.
Citation - WoS: 4
Citation - Scopus: 4
Adaptation of Symbolic Discrete Control Synthesis for Energy-Efficient Multi-Pocket Milling
(MDPI, 2024) Caska, Serkan; Ozbaltan, Mete
In engineering, cost minimization, especially in Computer Numerical Control (CNC) machining like pocket milling, is crucial. Existing tool path definition software often lacks optimization, particularly at critical starting and ending points. This study optimizes CNC machine tool paths for energy-efficient multi-pocket milling, utilizing the Symbolic Discrete Control Synthesis (SDCS) method for formal correctness. In our work, the tool path generation is formulated as a traveling salesman problem. We introduce a modeling framework to adapt SDCS to multi-pocket-milling processes, aiming to enhance precision and efficiency for potential cost savings, including energy and time, in engineering applications. This study reports experimental and comparative results, where comparative evaluations were made using metaheuristic algorithms. Our proposed approach improves CNC machining processes for multi-pocket milling. We experimentally evaluate our control algorithms and demonstrate and validate our approach through case studies.
Citation - WoS: 1
Citation - Scopus: 1
Adaptive Event Triggered Control of Nonholonomic Mobile Robots
(Springer International Publishing AG, 2021) Guzey, Mehmet
In this paper, the design of adaptive regulation control of mobile robots in the presence of uncertain robot dynamics and with event-based feedback is presented. Two-layer neural networks (NN) are utilized to represent the uncertain nonlinear dynamics of the mobile robots, which is subsequently employed to generate the control torque with event-sampled measurement update. Relaxing the perfect velocity tracking assumption, control torque is designed to minimize the velocity tracking error, by explicitly taking into account the dynamics of the robot. The Lyapunov's stability method is utilized to develop an event-sampling condition and to demonstrate the regulation performance of the mobile robot. Finally, simulation results are presented to verify theoretical claims and to demonstrate the reduction in the computations with event-sampled control execution.
Citation - Scopus: 1
Adaptive Event-Triggered Regulation Control of Nonholonomic Mobile Robots
(Springer, 2020) Guzey, M.
In this chapter, the design of adaptive-regulation control of mobile robots (MR) in the presence of uncertain MR dynamics with event-based feedback is provided. Two layer neural-networks (NN) are utilized to represent the uncertain dynamics of the MR which is subsequently employed to generate the control torque with event-sampled measurement update. By relaxing the perfect velocity tracking assumption, control torque is developed to minimize the velocity tracking errors, by explicitly taking into account the dynamics of the MR. The Lyapunov’s method is utilized to develop an event-sampling condition and to demonstrate the regulation error performance of the MR. At the end of the chapter, simulation results are given to verify our theoretical claims. © Springer Nature Singapore Pte Ltd. 2020.
An Advanced Lifetime Measurement Method by Square Wave Excitation and Lock-In Amplifier
(IOP Publishing Ltd, 2021) Çodur, M.M.; Yerci˙, S.
Achieving the radiation decay rate with high accuracy is crucial for all light emitters and absorbers. Therefore, a wide variety of measurement approaches have been developed for the measurement of radiation decay rate. Herein, we introduce a method to measure the decay rate with a common photoluminescence intensity setup without any modification. The square wave created by simply turning the output of the laser on and off is used as the excitation waveform, and a lock-in amplifier is used to gather the detected signal, making it a cost-effective way. Moreover, the constructed method can be used in a wide range of frequencies, detection voltages, and lifetimes. Finally, we applied the derived method to measure the photoluminescence lifetime of the first excited state of erbium ions in upconverting erbium-ytterbium silicate samples. © Published under licence by IOP Publishing Ltd.
Citation - WoS: 5
Citation - Scopus: 3
Alleviation of LPS-Induced Acute Lung Injury by Propolis-Based Nanocomposites Through the TLR4/NFκB and P2X7/Akt Pathways: Randomized-Controlled Experimental Study
(Pergamon-Elsevier Science Ltd, 2025) Ustundag, Hilal; Kara, Adem; Tas, Necip Gokhan; Kalindemirtas, Ferdane Danisman; Kurt, Nezahat; Erbas, Elif; Kariper, Ishak Afsin
Sepsis-associated acute lung injury continues to pose a significant medical challenge with substantial morbidity and mortality rates. In this study, we investigated the therapeutic potential of propolis-based treatments and their nanocomposites in modulating inflammation and apoptosis using a lipopolysaccharide (LPS)-induced rat model of sepsis. Forty-two Sprague-Dawley rats were divided into seven groups (n = 6): control, LPS (5 mg/kg, i. p.), LPS + Propolis (100 mg/kg, i.p.), LPS + NanoPropolis (100 mg/kg, i.p.), LPS + silver nanoparticles propolis (AgNPsPro) (50 mg/kg), and a negative propolis group (100 mg/kg, i.p.). The rats were assessed for inflammatory, oxidative stress, and apoptotic markers through Western blot, histopathological analyses, and biochemical measurements. The LPS group exhibited significantly higher levels of pro-inflammatory cytokines (IL-1 beta, TNF-alpha) and the systemic infection marker presepsin (PRSN) in blood, as well as the oxidative stress marker malondialdehyde (MDA) in lung tissue. The treatment groups, particularly LPS + AgNPsPro, showed significant reductions in these markers, with decreased levels of MDA, IL-1 beta, TNF-alpha, NF-kappa B, and TLR4, and increased GSH content in lung tissue (p < 0.05). The anti-apoptotic protein BCL-2 was upregulated, while proapoptotic BAX expression was reduced, indicating enhanced cell survival. The P2X7 receptor, a key inflammation regulator, and the AKT signaling pathway, involved in cell survival, were positively modulated by the treatments. Histopathological findings corroborated these results, showing less lung tissue damage. In conclusion, propolisbased treatments, especially in combination with nanoparticles, demonstrate therapeutic potential in reducing inflammation, oxidative stress, and apoptosis in sepsis-induced lung injury.
Alleviation of LPS-Induced Acute Lung Injury by Propolis-Based Nanocomposites Through the TLR4/NFκB and P2X7/Akt Pathways: Randomized-Controlled Experimental Study (Vol 258, 108330, 2025)
(Pergamon-Elsevier Science Ltd, 2025) Ustundag, Hilal; Kara, Adem; Tas, Necip Gokhan; Kalindemirtas, Ferdane Danisman; Kurt, Nezahat; Erbas, Elif; Kariper, Ishakshak Afsin
Citation - WoS: 41
Citation - Scopus: 47
The Altered Promoter Methylation of Oxytocin Receptor Gene in Autism
(Taylor & Francis Ltd, 2016) Yuksel, Mine Elagoz; Yuceturk, Betul; Karatas, Omer Faruk; Ozen, Mustafa; Dogangung, Burak
Autism spectrum disorder (ASD) is one of the lifelong existing disorders. Abnormal methylation status of gene promoters of oxytonergic system has been implicated as among the etiologic factors of ASDs. We, therefore, investigated the methylation frequency of oxytocin receptor gene (OXTR) promoter from peripheral blood samples of children with autistic features. Our sample includes 66 children in total (22-94 months); 27 children with ASDs according to the DSM-IV-TR and the Childhood Autism Rating Scale (CARS) and 39 children who do not have any autistic like symptoms as the healthy control group. We investigated the DNA methylation status of OXTR promoter by methylation specific enzymatic digestion of genomic DNA and polymerase chain reaction. A significant relationship has been found between ASDs and healthy controls for the reduction of methylation frequency of the regions MT1 and MT3 of OXTR. We could not find any association in the methylation frequency of MT2 and MT4 regions of OXTR. Although our findings indicate high frequency of OXTR promoter hypomethylation in ASDs, there is need for independent replication of the results for a bigger sample set. We expect that future studies with the inclusion of larger, more homogeneous samples will attempt to disentangle the causes of ASDs.
Citation - WoS: 4
Citation - Scopus: 7
Aluminium Phosphide-Induced Genetic and Oxidative Damages in Vitro: Attenuation by Laurus Nobilis L. Leaf Extract
(Wolters Kluwer Medknow Publications, 2013) Turkez, Hasan; Togar, Basak
Objective: The present investigation was undertaken to assess the protective effect of Laurus nobilis leaf extract (LNE) against aluminum phosphide (AIP)-induced genotoxic and oxidative damages stress in cultured human blood cells in the presence of a metabolic activator (S9 mix). Materials and Methods: Sister chromatid exchange (SCE) and chromosome aberration (CA) assays were used to assess AlP-induced genotoxicity and to establish the protective effects of LNE. In addition, we determined total antioxidant capacity (TAC) and total oxidative status (TOS) levels in AlP and LNE treated cultures for biomonitoring the oxidative alterations. Results: There was significant increases (P < 0.05) in both SCE and CA frequencies of cultures treated with AlP as compared to controls. Our results also showed that AlP (58 mg/l) caused oxidative stress by altering TAC and TOS levels. However, co-application of LNE (25, 50, 100 and 200 mg/l) and AlP resulted in decreases of SCE, CA rates and TOS level and increases of TAC level as compared to the group treated with AlP alone. Conclusion: The preventive role of LNE in alleviating AlP-induced DNA and oxidative damages was indicated for the first time in the present study.
Citation - WoS: 15
Citation - Scopus: 16
Aluminum Phosphide-Induced Genetic and Oxidative Damages in Rats: Attenuation by Laurus Nobilis Leaf Extract
(Sage Publications Inc, 2013) Turkez, Hasan; Togar, Basak
Aluminum phosphide (AlP) is a colorless, flammable, liquefied pesticide that is commonly used to control insects, nematodes, weeds, and pathogens in crops, forests, ornamental nurseries, and wood products. Early investigations of AlP-poisoned mammalian cells led to the proposed involvement of oxidative damage in its toxicity mechanism. Therefore, this study was aimed to evaluate the effect of Laurus nobilis (L) leaf extract (LNE) against AlP-induced genetic and oxidative damages in rats. Selected animals were assigned to four groups (n = 6), namely, group A: control (only distilled water is injected); group B: AlP (4 mg kg(-1) injected intraperitoneally (i.p.)); group C: LNE (200 mg kg(-1) injected i.p.), and group D: AlP plus LNE, respectively. The experimental period lasted for 14 successive days. Chromosomal aberrations (CAs) and micronucleus (MN) assay were used for monitoring genotoxic damage. In addition, biochemical parameters such as total antioxidant capacity (TAC) and total oxidative status (TOS) were examined in serum samples to determine oxidative damage. Our results indicated that AlP caused increase in CA and MN assay rates and alterations in TAC and TOS levels when compared with control group. On the contrary, LNE did not change the rates of both the analyzed cytogenetic end points and led to increase in TAC level. Moreover, we observed that LNE suppressed the genetic damage by AlP to bone marrow cells in vivo. Interestingly AlP-induced oxidative stress was also strongly reduced by LNE. The results of the present study indicated that the protective effect of LNE might be ascribable to its antioxidant and free radical scavenging properties.
Citation - WoS: 8
Citation - Scopus: 10
Ameliorative Effects of Docosahexaenoic Acid on the Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo in Cultured Rat Hepatocytes
(Sage Publications Inc, 2016) Turkez, Hasan; Geyikoglu, Fatime; Yousef, Mokhtar I.
The 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is an environmental contaminant toxicant that mediates carcinogenic effects associated with oxidative DNA damage. Docosahexaenoic acid (DHA) with antioxidant functions has many biochemical, cellular, and physiological functions for cells. The present study assessed, for the first time, the ameliorative effect of DHA in alleviating the toxicity of TCDD on primary cultured rat hepatocytes (HEPs). In vitro, isolated HEPs were incubated with TCDD (5 and 10 M) in the presence and absence of DHA (5, 10, and 20 M) for 48 h. The cell viability was detected by 3-(4,5-dimethylthiazol-2-yl) 2,5diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) release. DNA damage was analyzed by liver micronucleus assay and 8-oxo-2-deoxyguanosine (8-OH-dG) level. In addition, total antioxidant capacity (TAC) and total oxidative stress (TOS) were assessed to determine the oxidative injury in HEPs. The results of MTT and LDH assays showed that TCDD decreased cell viability but not DHA. On the basis of increasing treatment concentrations, the dioxin caused significant increases of micronucleated HEPs and 8-OH-dG as compared to control culture. TCDD also led to significant increases in TOS content. On the contrary, in cultures treated with DHA, the level of TAC was significantly increased during treatment in a concentration-dependent fashion. DHA showed therapeutic potential against TCDD-mediated cell viability and DNA damages. As conclusion, this study provides the first evidence that DHA has protective effects against TCDD toxicity on primary cultured rat hepatocytes.
Citation - WoS: 8
Citation - Scopus: 9
Analysis of Light Scattering from Anisotropic Particles Using FDTD Method
(Taylor & Francis Ltd, 2019) Kaburcuk, Fatih; Duman, Caglar
Light scattering from small anisotropic particles has important interests in the applications of meteorology, oceanography, communication, and nanotechnology. In this paper, the light scattering from uniaxial and biaxial small particles is investigated using the anisotropy formulation based on the finite-difference time-domain (FDTD) method, although this method has been used to analyse the isotropic particles. The obtained light scattering solutions from the isotropic small particle using the FDTD method are compared with the exact solutions obtained by Mie theory. The effects of the anisotropy and the rotation of optical axes on the light scattering are also investigated. Numerical results show that the distribution of scattered light is strongly affected by the anisotropy of the particles. Therefore, the anisotropy of the scattering particles is an important factor in several applications such as free-space optical communications.
Citation - WoS: 13
Citation - Scopus: 14
An Analysis of Mandibular Symphyseal Graft Sufficiency for Alveolar Cleft Bone Grafting
(Lippincott Williams & Wilkins, 2017) Kilinc, Adnan; Saruhan, Nesrin; Ertas, Umit; Korkmaz, Ismail Hakki; Kaymaz, Irfan
The purpose of this study was to evaluate the sufficiency of the mandibular symphysis as a donor site for unilateral and bilateral alveolar grafting, measuring both the alveolar cleft volume and maximum bone graft volume that can be harvested from the mandibular symphysis using 3-dimensional computed tomography (CT) and software in children and adults. Computed tomography data obtained from 20 unilateral and bilateral cleft lip palates patients in the preoperative period were used in this study. The patients were divided into 2 groups: children (female, n = 5; male, n = 5) and adults (female, n = 5; male, n = 5). The required bone graft volume for grafting and the maximum bone graft volume that can be harvested from the mandibular symphysis were measured based on cone beam CT data and software. The average required bone graft volume (cleft volume) for unilateral alveolar grafting was 963.51 +/- 172.31 mm(3) in the children and 1001.21 +/- 268.16 mm(3) in the adults. The average required bone graft volume for bilateral alveolar grafting was 1457.82 +/- 148.18 mm(3) in the children and 2189.59 +/- 600.97 mm(3) in the adults. The average the mandibular symphysis bone graft volume was 819.29 +/- 330.85 mm(3) in the children and 2164.9 +/- 1095.86 mm(3) in the adults. The results demonstrated that the mandibular symphysis region provided an adequate bone volume for alveolar grafting in adults with unilateral alveolar clefts. However, it is difficult to standardize these results, due to cleft volume and graft volume that could be harvested from the mandibular symphysis are highly variable among individuals.
Citation - Scopus: 1
Analysis of miRNA in Plants by Next Generation Sequencing Technology
(Centenary University, 2016) Eren, A.H.; İlhan, E.; Inal, B.
miRNAs (microRNAs) find in the intron sites of the genes. miRNAs are produced from their gene region and non-coding RNAs. In plants, the production of miRNAs that consist of 21–24 nucleotides in length starts in nucleus and continues coordinately in cytoplasm. miRNAs help to protect the integrity of genome duo to define expression levels of genes related to development and stress in plant. In this way, miRNAs are considered to defend 30% of eukaryotic genomes. There are a few types of small RNAs such as miRNAs, siRNAs, phasiRNAs and NAT – siRNAs in plants. Various methods such as Northern Blotting, Flow cytometer, cloning, qRT – PCR, Microarray, sequencing and RNA-seq (transcriptome profiling) were developed to detect and discover functions of these small RNAs. Species and tissue specific miRNAs can be easily identified with this method called as miRNA-seq, one of the Next Generation Sequencing methods. In miRNA studies, the algorithms such as miRDeep-Seq, miRanalyzer, miRCat, miRExpress, miRTRAP can be used. This review summarizes about up to date applications of next generation seqeuncing technologies in miRNA-seq studies. © 2016, Centenary University. All rights reserved.
Citation - WoS: 3
Citation - Scopus: 2
Analysis of the Descriptive Characteristics of Female Victims of Violence Applying to the Domestic Violence Office of a Court of Law
(Elsevier Sci Ltd, 2023) Yildirim, Nilufer; Toraman, Aynur Uysal
Introduction and Purpose: Intimate partner violence is a worldwide problem violating fundamental human rights. The aim of this study was to analyse the sociodemographic characteristics of women who have experienced intimate partner violence, the type and prevalence of violence, the mechanisms of injury as determined by forensic reports, the characteristics of the perpetrator, and the women's statements.Materials and methods: This was a single-site descriptive study conducted at the Office of Domestic Violence and Violence Against Women of a Court of Law located in the city of Izmir in western Turkey. The researchers reviewed forensic medicine case reports and prosecutorial writs in this office's files for women over 18 who had experienced violence over the period 2016-2019. The study sample consisted of the judicial application files of women who had experienced intimate partner violence and met the inclusion criteria (n = 350). The data in the files were entered into a standard form prepared by the researchers according to the file content. Written permission was obtained from the Ministry of Justice and Ege University Ethics Committee, and the verbal consent of the Prosecuting Officer was also obtained for the research.Results: The ages of the women ranged from 19 to 80 years (mean age 35, SD 9.6), with 43.1% ranging from 30 to 39 years old. Of the women, 46.6% had a maximum primary school education, and 65.4% were homemakers. Incidents of intimate partner violence mainly occurred at home for 89.1% of women. A combination of verbal and physical violence was the most frequent form of violence affecting 303 women (83.4% of cases). The facial area was predominantly the target of attack for 59 (16.9%) victims, only the upper extremity for 55 (15.7%) and the face and upper extremity for 36 (10.2%) women. The statements of the victims of violence who described their experience were evaluated, and it was determined that the reasons for the emergence of violence were frequently alcohol and substance use, financial problems, jealousy, sexual problems, communication problems, and cheating.Conclusion and Suggestions: Most of the women in the study who had applied to law enforcement due to intimate partner violence were victims of physical violence. The descriptive information obtained from these files con-stitutes essential data for health professionals in their efforts to deliver primary healthcare to women who are victims of intimate partner violence. Health professionals can provide immediate protection by identifying women at high risk of violence, monitoring them more frequently, and activating the support mechanisms they need.
Citation - Scopus: 2
Analysis of the Factors Affecting Credit Card Use and Online Shopping Attitudes of Households in Turkey with the Bivariate Probit Model
(Inderscience Publishers, 2022) Çebi-Karaaslan, K.
This study investigated the factors that affect households’ credit card usage and online shopping attitudes. The dataset of the study was provided by the Turkey Statistical Institute. The bivariate probit regression model was used in the work. As a result, it was determined that demographic, economic and environmental factors have significant effects on credit card use and online shopping attitudes. Knowing the characteristics of credit card and internet shopping users in light of the results, helps banks and shopping sites to determine advertising strategies. It will enable policy makers to raise awareness and protect the consumer and to implement them to stimulate the economy. © © 2022 Inderscience Enterprises Ltd.
Analysis of the Relationship Between Self-Neglect and Spiritual Well-Being in Older Adults
(Routledge Journals, Taylor & Francis Ltd, 2025) Ozturk, Zeynep; Turan, Gulcan Bahcecioglu; Donmez, Orhun
This study was conducted to examine the relationship between self-neglect and spiritual well-being in older adults. This descriptive, cross-sectional and correlational study was conducted with 232 older adults. Data were collected using the "Elder Self-Neglect Scale (ESNS)" and "Spiritual Well-Being Scale (FACIT Sp-12)." It was found that 54% of the participants were female, 86% were married, 35% were primary school graduates, 66% lived in the district, 27% had hypertension, 54% had income equal to expenses, and 76% were retired. Mean ESNS total score of older adults was 102.83 +/- 24.82 and mean FACIT Sp-12 total score was 33.73 +/- 9.70. FACIT Sp-12 total score was found to be a significant negative predictor of the dependent variable ESNS total score (p < .05). In this study, it was determined that the spiritual well-being levels of older adults were above average and their self-neglect levels were low. It was determined that as the spiritual well-being levels of older adults increased, their self-neglect levels decreased.
An Ancient Mourning Ritual: The Biological Functions of Mourning and Its Place in Social Memory
(Milli Folklor Dergisi, 2025) Alan, S.
The practice of lamentation is a form of social expression that enables personal grief to be transferred into collective memory while supporting the continuity of oral tradition. By facilitating the communal sharing of loss, this tradition reinforces emotional solidarity and contributes to cultural sustainability. Lament narratives transcend private sorrow and construct a shared space of empathy, transforming subjective experiences of tragedy into elements of public memory. This study focuses on the biological dimensions of lament, analyzing the interaction between bodily gestures and verbal expression within its socio-cultural functions through an interdisciplinary approach that incorporates folklore, psychology, medicine (specially neurophysiology and immunology), cultural anthropology, and performance theory. The ritual performance structure, physiological reflections, and social functions of lament have been systematically examined. The first section discusses the biological mechanisms activated during lamentation. Bodily movements such as chest-beating, hair-pulling, and bowing stimulate the thymus gland and enhance immune response, thereby increasing physical resilience. These gestures regulate cortisol levels via the parasympathetic nervous system, mitigating the physiological impact of stress. Intense emotional expression enables the release of accumulated negative energy and has been observed to play a key role in preventing chronic stress-related disorders. The findings show that lament serves not merely as a psychological outlet but also as a multifaceted process contributing to somatic healing. By aiding the reconstruction of mental and physiological balance after traumatic loss, lament activates the body’s natural defense systems and supports holistic well-being. The second section explores the socio-cultural significance of lament. It has been observed that these expressions embed historical traumas into shared memory and transform personal recollection into collective narrative, forming the structural foundation of oral cultural texts. In the Anatolian tradition, laments document not only mourning but also broader historical experiences, thus contributing to the preservation of communal identity and oral historiography. They serve as tools for intergenerational transmission of values, belief systems, and cultural norms, reinforcing collective continuity. The third section analyzes the interplay of language and embodiment within the lament performance. Rhythmic verbal repetitions combine with codified physical gestures to form a coherent ritual narrative. The ceremonial texture enables personal sorrow to be aesthetically and symbolically reconfigured in public space, turning mourning into a shared social enactment. This performative structure integrates private emotional disruption into the communal flow of affect. Examined in relation to its biological regulation and neural impact, the discourse of mourning is shown to contribute significantly to both psychosomatic restoration and memory-making. Findings affirm that lamentation functions not solely as a traditional ritual, but as a cultural mechanism that generates healing and shapes collective remembrance. © 2025, Milli Folklor Dergisi. All rights reserved.
Citation - WoS: 16
Citation - Scopus: 20
Anti-Genotoxic Role of Eicosapentaenoic Acid Against Imazalil-Induced DNA Damage in Vitro
(Sage Publications Inc, 2013) Turkez, Hasan; Aydin, Elanur
Eicosapentaenoic acid (EPA) is a polyunsaturated n-3 fatty acid and is essential to the health of mammals. Recent data show that EPA can act as anti-mutagenic agent. On the other hand, pesticides comprise a new and important class of environmental pollutants nowadays. Imazalil (IMA), a commonly used fungicide in both agricultural and clinical domains is suspected to produce very serious toxic effects in vertebrates. The present study investigated the anti-genotoxic effect of EPA against the genotoxic damage induced by IMA on cultured human lymphocytes using chromosomal aberration (CA) and micronucleus (MN) tests as cytogenetic endpoints. Peripheral blood cells were treated in vitro with varying concentrations of EPA (2.5, 5, 10, 20 and 40 g/ml), tested in combination with IMA (336 g/ml). Our results revealed that the rates of CAs and MNs in lymphocytes were significantly (p < 0.05) increased by IMA as compared to the controls. The results also showed that EPA alone was not genotoxic. Moreover, when combined with IMA treatment, EPA reduced the frequencies of CAs and MNs. A clear dose-dependent decrease in the genotoxic damage of IMA was observed, suggesting a genoprotective role of EPA. In conclusion, our data may have an important application for the protection of cultured human lymphocyte from the genetic damage and repercussions induced by agricultural and industrial chemicals hazardous in people.
Citation - WoS: 67
Citation - Scopus: 69
Anticancer and Antioxidant Properties of Terpinolene in Rat Brain Cells
(Inst Medical Research & Occupational Health, 2013) Aydin, Elanur; Turkez, Hasan; Tasdemir, Sener
Terpinolene (TPO) is a natural monoterpene present in essential oils of many aromatic plant species. Although various biological activities of TPO have been demonstrated, its neurotoxicity has never been explored. In this in vitro study we investigated TPO's antiproliferative and/or cytotoxic properties using the 3-(4,5-dimethylthiazol-2-y1)-2,5 diphenyltetrazolium bromide (MTT) test, genotoxic damage potential using the single-cell gel electrophoresis (SCGE), and oxidative effects through total antioxidant capacity (TAC) and total oxidative stress (TOS) in cultured primary rat neurons and N2a neuroblastoma cells. Dose-dependent effects of TPO (at 10 mg L-1, 25 mg L-1, 50 mg L-1, 100 mg L-1, 200 mg L-1, and 400 mg L-1) were tested in both cell types. Significant (P<0.05) decrease in cell proliferation were observed in cultured primary rat neurons starting with the dose of 100 mg L-1 and in N2a neuroblastoma cells starting with 50 mg L-1. TPO was not genotoxic in either cell type. In addition, TPO treatment at 10 mg L-1, 25 mg L-1, and 50 mg L-1 increased TAC in primary rat neurons, but not in N2a cells. However, at concentrations above 50 mg L-1 it increased TOS in both cell types. Our findings clearly demonstrate that TPO is a potent antiproliferative agent for brain tumour cells and may have potential as an anticancer agent, which needs to be further studied.

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