* Akademik Arşiv

Permanent URI for this communityhttps://hdl.handle.net/20.500.14901/693

Browse

Now showing 1 - 20 of 448

Citation - WoS: 3
Citation - Scopus: 4
Acute Effects of Three Different Stretching Techniques on Hamstring Flexibility in Professional Football Players
(Gdansk Univ Physical Education & Sport, 2023) Erol, Erkan; Yildiz, Ramazan; Yildiz, Aye; Dogan, Fatih Emre; Elbasan, Buelent
Introduction: Hamstring injuries are common in physically active people and athletes. Lack of hamstring flexibility is the one of the most important characteristics of hamstring injuries in athletes. The aim of this study was to investigate effects of three different stretching techniques on hamstring flexibility in professional football players. Materials and Methods: Fifty-five male football players between the ages of 18-20 years old were involved in the study. The participants were divided into 3 groups: static stretching, neuromobilization and Mulligan traction straight leg raise (TSLR), respectively. Each participant's dominant lower extremity was assessed with a straight leg raise test (SLR) using a goniometer before and after the intervention. Results: There was a significant difference in the SLR range of motion before and after the intervention in all groups. There was no difference in the range of SLR between the groups at the beginning and at the end. However, the increase in the SLR range of motion was significantly higher in the Mulligan TSLR group. Conclusions: It was concluded that all 3 stretching techniques are effective in increasing hamstring flexibility in football players. However, the Mulligan TSLR technique could provide a greater increase in the range of motion than other techniques.
Citation - WoS: 4
Citation - Scopus: 4
Adaptation of Symbolic Discrete Control Synthesis for Energy-Efficient Multi-Pocket Milling
(MDPI, 2024) Caska, Serkan; Ozbaltan, Mete
In engineering, cost minimization, especially in Computer Numerical Control (CNC) machining like pocket milling, is crucial. Existing tool path definition software often lacks optimization, particularly at critical starting and ending points. This study optimizes CNC machine tool paths for energy-efficient multi-pocket milling, utilizing the Symbolic Discrete Control Synthesis (SDCS) method for formal correctness. In our work, the tool path generation is formulated as a traveling salesman problem. We introduce a modeling framework to adapt SDCS to multi-pocket-milling processes, aiming to enhance precision and efficiency for potential cost savings, including energy and time, in engineering applications. This study reports experimental and comparative results, where comparative evaluations were made using metaheuristic algorithms. Our proposed approach improves CNC machining processes for multi-pocket milling. We experimentally evaluate our control algorithms and demonstrate and validate our approach through case studies.
Citation - WoS: 6
Citation - Scopus: 10
Affective States Classification Performance of Audio-Visual Stimuli from EEG Signals with Multiple-Instance Learning
(TÜBİTAK Scientific & Technological Research Council Turkey, 2022) Dasdemir, Yaar; Ozakar, Rustem
Throughout various disciplines, emotion recognition continues to be an essential subject of study. With the advancement of machine learning methods, accurate emotion recognition from different data modalities (facial images, brain EEG signals) has become possible. Success of EEG-based emotion recognition systems depends on efficient feature extraction and pre/postprocessing of signals. Main objective of this study is to analyze the efficacy of multiple-instance learning (MIL) on postprocessing features of EEG signals using three different domains (time, frequency, time-frequency) for human emotion classification. Methods and results are presented for single-trial classification of valence (V), arousal (A), and dominance (D) ratings from EEG signals obtained with audio (A), video (V), and audio-video (AV) stimulus using alpha, beta and gamma bands. High accuracy was observed with both binary and multiclass classification of the AV stimulus. Findings in this study suggest that MIL applied on frequency features yields efficient results on EEG emotion recognition.
Citation - WoS: 5
Citation - Scopus: 3
Alleviation of LPS-Induced Acute Lung Injury by Propolis-Based Nanocomposites Through the TLR4/NFκB and P2X7/Akt Pathways: Randomized-Controlled Experimental Study
(Pergamon-Elsevier Science Ltd, 2025) Ustundag, Hilal; Kara, Adem; Tas, Necip Gokhan; Kalindemirtas, Ferdane Danisman; Kurt, Nezahat; Erbas, Elif; Kariper, Ishak Afsin
Sepsis-associated acute lung injury continues to pose a significant medical challenge with substantial morbidity and mortality rates. In this study, we investigated the therapeutic potential of propolis-based treatments and their nanocomposites in modulating inflammation and apoptosis using a lipopolysaccharide (LPS)-induced rat model of sepsis. Forty-two Sprague-Dawley rats were divided into seven groups (n = 6): control, LPS (5 mg/kg, i. p.), LPS + Propolis (100 mg/kg, i.p.), LPS + NanoPropolis (100 mg/kg, i.p.), LPS + silver nanoparticles propolis (AgNPsPro) (50 mg/kg), and a negative propolis group (100 mg/kg, i.p.). The rats were assessed for inflammatory, oxidative stress, and apoptotic markers through Western blot, histopathological analyses, and biochemical measurements. The LPS group exhibited significantly higher levels of pro-inflammatory cytokines (IL-1 beta, TNF-alpha) and the systemic infection marker presepsin (PRSN) in blood, as well as the oxidative stress marker malondialdehyde (MDA) in lung tissue. The treatment groups, particularly LPS + AgNPsPro, showed significant reductions in these markers, with decreased levels of MDA, IL-1 beta, TNF-alpha, NF-kappa B, and TLR4, and increased GSH content in lung tissue (p < 0.05). The anti-apoptotic protein BCL-2 was upregulated, while proapoptotic BAX expression was reduced, indicating enhanced cell survival. The P2X7 receptor, a key inflammation regulator, and the AKT signaling pathway, involved in cell survival, were positively modulated by the treatments. Histopathological findings corroborated these results, showing less lung tissue damage. In conclusion, propolisbased treatments, especially in combination with nanoparticles, demonstrate therapeutic potential in reducing inflammation, oxidative stress, and apoptosis in sepsis-induced lung injury.
Alleviation of LPS-Induced Acute Lung Injury by Propolis-Based Nanocomposites Through the TLR4/NFκB and P2X7/Akt Pathways: Randomized-Controlled Experimental Study (Vol 258, 108330, 2025)
(Pergamon-Elsevier Science Ltd, 2025) Ustundag, Hilal; Kara, Adem; Tas, Necip Gokhan; Kalindemirtas, Ferdane Danisman; Kurt, Nezahat; Erbas, Elif; Kariper, Ishakshak Afsin
Almost Ultrafilters and Compactifications
(Chiang Mai Univ, 2014) Elmali, Ceren Sultan
If Xis a discrete topological space, the points of its Stone-Cech compactification beta X can be regarded as ultrafilters on X, and this fact is a useful tool in analysing the properties of beta X The purpose of this paper is to describe the compactification. of X of a uniform space in terms of the concept of almost ultrafilters. We describe the topological space X* and we investigate conditions under which S* will be a semigroup compactification if S is a semigroup which has a uniform structure. These conditions will always hold if S is a topological group, and in this case our compactification S* coincides with S-LUC.
Citation - WoS: 41
Citation - Scopus: 47
The Altered Promoter Methylation of Oxytocin Receptor Gene in Autism
(Taylor & Francis Ltd, 2016) Yuksel, Mine Elagoz; Yuceturk, Betul; Karatas, Omer Faruk; Ozen, Mustafa; Dogangung, Burak
Autism spectrum disorder (ASD) is one of the lifelong existing disorders. Abnormal methylation status of gene promoters of oxytonergic system has been implicated as among the etiologic factors of ASDs. We, therefore, investigated the methylation frequency of oxytocin receptor gene (OXTR) promoter from peripheral blood samples of children with autistic features. Our sample includes 66 children in total (22-94 months); 27 children with ASDs according to the DSM-IV-TR and the Childhood Autism Rating Scale (CARS) and 39 children who do not have any autistic like symptoms as the healthy control group. We investigated the DNA methylation status of OXTR promoter by methylation specific enzymatic digestion of genomic DNA and polymerase chain reaction. A significant relationship has been found between ASDs and healthy controls for the reduction of methylation frequency of the regions MT1 and MT3 of OXTR. We could not find any association in the methylation frequency of MT2 and MT4 regions of OXTR. Although our findings indicate high frequency of OXTR promoter hypomethylation in ASDs, there is need for independent replication of the results for a bigger sample set. We expect that future studies with the inclusion of larger, more homogeneous samples will attempt to disentangle the causes of ASDs.
Citation - WoS: 15
Citation - Scopus: 16
Aluminum Phosphide-Induced Genetic and Oxidative Damages in Rats: Attenuation by Laurus Nobilis Leaf Extract
(Sage Publications Inc, 2013) Turkez, Hasan; Togar, Basak
Aluminum phosphide (AlP) is a colorless, flammable, liquefied pesticide that is commonly used to control insects, nematodes, weeds, and pathogens in crops, forests, ornamental nurseries, and wood products. Early investigations of AlP-poisoned mammalian cells led to the proposed involvement of oxidative damage in its toxicity mechanism. Therefore, this study was aimed to evaluate the effect of Laurus nobilis (L) leaf extract (LNE) against AlP-induced genetic and oxidative damages in rats. Selected animals were assigned to four groups (n = 6), namely, group A: control (only distilled water is injected); group B: AlP (4 mg kg(-1) injected intraperitoneally (i.p.)); group C: LNE (200 mg kg(-1) injected i.p.), and group D: AlP plus LNE, respectively. The experimental period lasted for 14 successive days. Chromosomal aberrations (CAs) and micronucleus (MN) assay were used for monitoring genotoxic damage. In addition, biochemical parameters such as total antioxidant capacity (TAC) and total oxidative status (TOS) were examined in serum samples to determine oxidative damage. Our results indicated that AlP caused increase in CA and MN assay rates and alterations in TAC and TOS levels when compared with control group. On the contrary, LNE did not change the rates of both the analyzed cytogenetic end points and led to increase in TAC level. Moreover, we observed that LNE suppressed the genetic damage by AlP to bone marrow cells in vivo. Interestingly AlP-induced oxidative stress was also strongly reduced by LNE. The results of the present study indicated that the protective effect of LNE might be ascribable to its antioxidant and free radical scavenging properties.
Citation - WoS: 8
Citation - Scopus: 10
Ameliorative Effects of Docosahexaenoic Acid on the Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo in Cultured Rat Hepatocytes
(Sage Publications Inc, 2016) Turkez, Hasan; Geyikoglu, Fatime; Yousef, Mokhtar I.
The 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is an environmental contaminant toxicant that mediates carcinogenic effects associated with oxidative DNA damage. Docosahexaenoic acid (DHA) with antioxidant functions has many biochemical, cellular, and physiological functions for cells. The present study assessed, for the first time, the ameliorative effect of DHA in alleviating the toxicity of TCDD on primary cultured rat hepatocytes (HEPs). In vitro, isolated HEPs were incubated with TCDD (5 and 10 M) in the presence and absence of DHA (5, 10, and 20 M) for 48 h. The cell viability was detected by 3-(4,5-dimethylthiazol-2-yl) 2,5diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) release. DNA damage was analyzed by liver micronucleus assay and 8-oxo-2-deoxyguanosine (8-OH-dG) level. In addition, total antioxidant capacity (TAC) and total oxidative stress (TOS) were assessed to determine the oxidative injury in HEPs. The results of MTT and LDH assays showed that TCDD decreased cell viability but not DHA. On the basis of increasing treatment concentrations, the dioxin caused significant increases of micronucleated HEPs and 8-OH-dG as compared to control culture. TCDD also led to significant increases in TOS content. On the contrary, in cultures treated with DHA, the level of TAC was significantly increased during treatment in a concentration-dependent fashion. DHA showed therapeutic potential against TCDD-mediated cell viability and DNA damages. As conclusion, this study provides the first evidence that DHA has protective effects against TCDD toxicity on primary cultured rat hepatocytes.
Citation - WoS: 13
Citation - Scopus: 14
An Analysis of Mandibular Symphyseal Graft Sufficiency for Alveolar Cleft Bone Grafting
(Lippincott Williams & Wilkins, 2017) Kilinc, Adnan; Saruhan, Nesrin; Ertas, Umit; Korkmaz, Ismail Hakki; Kaymaz, Irfan
The purpose of this study was to evaluate the sufficiency of the mandibular symphysis as a donor site for unilateral and bilateral alveolar grafting, measuring both the alveolar cleft volume and maximum bone graft volume that can be harvested from the mandibular symphysis using 3-dimensional computed tomography (CT) and software in children and adults. Computed tomography data obtained from 20 unilateral and bilateral cleft lip palates patients in the preoperative period were used in this study. The patients were divided into 2 groups: children (female, n = 5; male, n = 5) and adults (female, n = 5; male, n = 5). The required bone graft volume for grafting and the maximum bone graft volume that can be harvested from the mandibular symphysis were measured based on cone beam CT data and software. The average required bone graft volume (cleft volume) for unilateral alveolar grafting was 963.51 +/- 172.31 mm(3) in the children and 1001.21 +/- 268.16 mm(3) in the adults. The average required bone graft volume for bilateral alveolar grafting was 1457.82 +/- 148.18 mm(3) in the children and 2189.59 +/- 600.97 mm(3) in the adults. The average the mandibular symphysis bone graft volume was 819.29 +/- 330.85 mm(3) in the children and 2164.9 +/- 1095.86 mm(3) in the adults. The results demonstrated that the mandibular symphysis region provided an adequate bone volume for alveolar grafting in adults with unilateral alveolar clefts. However, it is difficult to standardize these results, due to cleft volume and graft volume that could be harvested from the mandibular symphysis are highly variable among individuals.
Citation - WoS: 8
Citation - Scopus: 10
Analysis of Mixed Convection Heat Transfer of Nanofluids in a Minichannel for Aiding and Opposing Flow Conditions
(Springer, 2019) Manay, Eyuphan; Mandev, Emre; Temiz, Resul Onder
In this study, mixed convection heat transfer characteristics of nanofluid flow in a circular minichannel were investigated experimentally. The effects of the particle volume ratio (0, 0.25, and 0.75%) and Reynolds number (200-60) on heat transfer by mixed convection were investigated for aiding and opposing flow conditions. Water and water based SiO2 nanofluids were used as working fluid in a minichannel with a diameter of 1.9 mm, and constant heat flux was applied to the outer surface of the minichannel. Temperature-dependent thermophysical properties such as thermal conductivity and viscosity, obtained by experimental measurements, were used in heat transfer calculations. Pressure based numerical computations for all experimental cases were also made by using single phase approach. The results were analyzed separately for aiding flow condition in which secondary flows originating from natural convection are in the same direction with the forced flow, and for opposing flow condition in which secondary flows are in the opposite direction with forced flow. For detailed analysis of mixed convection heat transfer, temperature contours and velocity profiles were obtained by the numerical computations which were compared and validated with the experimental results. According to the data obtained, it was determined that the addition of nanoparticles into the pure water increased the Nusselt number by around 21-64% for the aiding flow, and 18-58% for the opposing flow. The heat transfer in the aiding flow was observed to be minimum 4% and maximum 16% higher in comparison with the opposing flow condition. It was concluded that the direction of secondary flows significantly affects heat transfer.
Citation - WoS: 3
Citation - Scopus: 2
Analysis of the Descriptive Characteristics of Female Victims of Violence Applying to the Domestic Violence Office of a Court of Law
(Elsevier Sci Ltd, 2023) Yildirim, Nilufer; Toraman, Aynur Uysal
Introduction and Purpose: Intimate partner violence is a worldwide problem violating fundamental human rights. The aim of this study was to analyse the sociodemographic characteristics of women who have experienced intimate partner violence, the type and prevalence of violence, the mechanisms of injury as determined by forensic reports, the characteristics of the perpetrator, and the women's statements.Materials and methods: This was a single-site descriptive study conducted at the Office of Domestic Violence and Violence Against Women of a Court of Law located in the city of Izmir in western Turkey. The researchers reviewed forensic medicine case reports and prosecutorial writs in this office's files for women over 18 who had experienced violence over the period 2016-2019. The study sample consisted of the judicial application files of women who had experienced intimate partner violence and met the inclusion criteria (n = 350). The data in the files were entered into a standard form prepared by the researchers according to the file content. Written permission was obtained from the Ministry of Justice and Ege University Ethics Committee, and the verbal consent of the Prosecuting Officer was also obtained for the research.Results: The ages of the women ranged from 19 to 80 years (mean age 35, SD 9.6), with 43.1% ranging from 30 to 39 years old. Of the women, 46.6% had a maximum primary school education, and 65.4% were homemakers. Incidents of intimate partner violence mainly occurred at home for 89.1% of women. A combination of verbal and physical violence was the most frequent form of violence affecting 303 women (83.4% of cases). The facial area was predominantly the target of attack for 59 (16.9%) victims, only the upper extremity for 55 (15.7%) and the face and upper extremity for 36 (10.2%) women. The statements of the victims of violence who described their experience were evaluated, and it was determined that the reasons for the emergence of violence were frequently alcohol and substance use, financial problems, jealousy, sexual problems, communication problems, and cheating.Conclusion and Suggestions: Most of the women in the study who had applied to law enforcement due to intimate partner violence were victims of physical violence. The descriptive information obtained from these files con-stitutes essential data for health professionals in their efforts to deliver primary healthcare to women who are victims of intimate partner violence. Health professionals can provide immediate protection by identifying women at high risk of violence, monitoring them more frequently, and activating the support mechanisms they need.
Citation - WoS: 12
Citation - Scopus: 12
Analysis of the Effect of Rock Salt on Grout’s Freeze-Thaw Strength with the Taguchi Method
(Elsevier, 2022) Zaimoglu, Ahmet Sahin; Kilic, Elif Ayik; Artuk, Fatih
Grouting is the injection of mixtures with high pressure into the voids, cracks, or pores that may exist in the soil. In the case of rock salt being present in the formations where the grouting is performed, studies on the effects of this salt presence on the freeze-thaw strength of the grout are rare in the literature. The Taguchi Method was used to assess the effect of rock salt on the freeze-thaw strength of grouts in this research (at 0, 50, and 100 freeze -thaw cycles). For the experimental design, a 3-parameter and a 4-level experimental design table were employed following the Taguchi Method. In the Taguchi Method, water/cement ratio (W/C), sand percentage (SP), and saturation percentage with rock salt (SPRS) were chosen as experimental parameters. The levels of these pa-rameters were determined as 0.8-1.0-1.2-1.4; 0%-25%-50%-100%; and 0%-25%-50%-100%, respectively. The freeze-thaw strength of the prepared grout samples at 0, 50, and 100 cycles were determined. As a result of the experiments, it was determined that the strength of 50 cycles of grout samples increased by up to the 2nd level (25%) of SPRS. The 0 and 100 freeze-thaw strength decreased with the increase in SPRS. Pertaining to all prepared grout samples, freeze-thaw strength decreased when W/C increased and rose as SP increased in general.
Analysis of the Relationship Between Self-Neglect and Spiritual Well-Being in Older Adults
(Routledge Journals, Taylor & Francis Ltd, 2025) Ozturk, Zeynep; Turan, Gulcan Bahcecioglu; Donmez, Orhun
This study was conducted to examine the relationship between self-neglect and spiritual well-being in older adults. This descriptive, cross-sectional and correlational study was conducted with 232 older adults. Data were collected using the "Elder Self-Neglect Scale (ESNS)" and "Spiritual Well-Being Scale (FACIT Sp-12)." It was found that 54% of the participants were female, 86% were married, 35% were primary school graduates, 66% lived in the district, 27% had hypertension, 54% had income equal to expenses, and 76% were retired. Mean ESNS total score of older adults was 102.83 +/- 24.82 and mean FACIT Sp-12 total score was 33.73 +/- 9.70. FACIT Sp-12 total score was found to be a significant negative predictor of the dependent variable ESNS total score (p < .05). In this study, it was determined that the spiritual well-being levels of older adults were above average and their self-neglect levels were low. It was determined that as the spiritual well-being levels of older adults increased, their self-neglect levels decreased.
Citation - WoS: 3
Citation - Scopus: 3
An Analytical Method to Visualize Higher Mode Effects on Yielding Cantilever Walls
(Wiley, 2021) Kazaz, Ilker
A nonlinear flexural plastic hinge developing at the base of the cantilever wall changes its dynamic properties. In such systems, contribution of higher modes to the total shear force response after yielding may be underestimated when they are assumed to prone same or some nonlinearity as the first mode. In order to calculate the modal properties of a cantilever walls developing a plastic hinge at the base after yielding, the fundamental analytical beam formulation is revisited, and by applying special boundary condition reflecting the nonlinear deformation at the fixed base, that is, plastic hinge rotation, a novel formulation that is able to track changes in the modal properties of yielding cantilever shear wall is derived. The modal properties are effectively calculated depending on the level of nonlinear plastic rotation demand. Derived formulation can be very useful in the analysis of higher mode effects on shear wall systems, where the essential understanding of dynamic shear force amplification in cantilever wall system lays in the variation of the systems dynamic modal properties extending from linear to nonlinear range. A mode superposition method using equivalent nonlinear modal single-degree-of-freedom (SDOF) systems is presented for the estimation of maximum shear force.
Citation - WoS: 16
Citation - Scopus: 20
Anti-Genotoxic Role of Eicosapentaenoic Acid Against Imazalil-Induced DNA Damage in Vitro
(Sage Publications Inc, 2013) Turkez, Hasan; Aydin, Elanur
Eicosapentaenoic acid (EPA) is a polyunsaturated n-3 fatty acid and is essential to the health of mammals. Recent data show that EPA can act as anti-mutagenic agent. On the other hand, pesticides comprise a new and important class of environmental pollutants nowadays. Imazalil (IMA), a commonly used fungicide in both agricultural and clinical domains is suspected to produce very serious toxic effects in vertebrates. The present study investigated the anti-genotoxic effect of EPA against the genotoxic damage induced by IMA on cultured human lymphocytes using chromosomal aberration (CA) and micronucleus (MN) tests as cytogenetic endpoints. Peripheral blood cells were treated in vitro with varying concentrations of EPA (2.5, 5, 10, 20 and 40 g/ml), tested in combination with IMA (336 g/ml). Our results revealed that the rates of CAs and MNs in lymphocytes were significantly (p < 0.05) increased by IMA as compared to the controls. The results also showed that EPA alone was not genotoxic. Moreover, when combined with IMA treatment, EPA reduced the frequencies of CAs and MNs. A clear dose-dependent decrease in the genotoxic damage of IMA was observed, suggesting a genoprotective role of EPA. In conclusion, our data may have an important application for the protection of cultured human lymphocyte from the genetic damage and repercussions induced by agricultural and industrial chemicals hazardous in people.
Citation - WoS: 29
Citation - Scopus: 30
Antibacterial Activity and Chemical Composition of Essential Oil Obtained from Nepeta Nuda Against Phytopathogenic Bacteria
(Taylor & Francis Inc, 2013) Gormez, Arzu; Bozari, Sedat; Yanmis, Derya; Gulluce, Medine; Agar, Guleray; Sahin, Fikrettin
The increase in agricultural production can be possible with the discovery and utilization of chemical compounds for plant disease control. However, indiscriminate use of chemical compounds against microorganisms causes many negative side effects on environment and mammalian health. Therefore, we aimed to find a new natural antimicrobial product from medicinal plants against phytopathogenic bacteria. In this study, the essential oil (EO) of Nepeta nuda was isolated by the hydrodistillation method and the chemical composition of the EO was analyzed by gas chromatography-mass spectrometry (GC-MS), as its biological activity was connected to its content. Then, the antibacterial property of EO was evaluated against twenty phytopathogenic bacteria. Nepeta contains 4a,7,7a-nepetalactone (18.10%), germacrene (15.68%), elemol (14.38%), -caryophyllene (8.81%), spathulenol (6.14%) and cubenol (5.10%) as major components. In conclusion, the present study is valuable for future investigations on controlling plant pathogenic bacteria.
Citation - WoS: 67
Citation - Scopus: 69
Anticancer and Antioxidant Properties of Terpinolene in Rat Brain Cells
(Inst Medical Research & Occupational Health, 2013) Aydin, Elanur; Turkez, Hasan; Tasdemir, Sener
Terpinolene (TPO) is a natural monoterpene present in essential oils of many aromatic plant species. Although various biological activities of TPO have been demonstrated, its neurotoxicity has never been explored. In this in vitro study we investigated TPO's antiproliferative and/or cytotoxic properties using the 3-(4,5-dimethylthiazol-2-y1)-2,5 diphenyltetrazolium bromide (MTT) test, genotoxic damage potential using the single-cell gel electrophoresis (SCGE), and oxidative effects through total antioxidant capacity (TAC) and total oxidative stress (TOS) in cultured primary rat neurons and N2a neuroblastoma cells. Dose-dependent effects of TPO (at 10 mg L-1, 25 mg L-1, 50 mg L-1, 100 mg L-1, 200 mg L-1, and 400 mg L-1) were tested in both cell types. Significant (P<0.05) decrease in cell proliferation were observed in cultured primary rat neurons starting with the dose of 100 mg L-1 and in N2a neuroblastoma cells starting with 50 mg L-1. TPO was not genotoxic in either cell type. In addition, TPO treatment at 10 mg L-1, 25 mg L-1, and 50 mg L-1 increased TAC in primary rat neurons, but not in N2a cells. However, at concentrations above 50 mg L-1 it increased TOS in both cell types. Our findings clearly demonstrate that TPO is a potent antiproliferative agent for brain tumour cells and may have potential as an anticancer agent, which needs to be further studied.
Citation - WoS: 9
Citation - Scopus: 9
Anticancer Effects of Novel NSAIDs Derivatives on Cultured Human Glioblastoma Cells
(Walter de Gruyter GmbH, 2021) Ozdemir, Ozlem; Marinelli, Lisa; Cacciatore, Ivana; Ciulla, Michele; Emsen, Bugrahan; Di Stefano, Antonio; Turkez, Hasan
Several epidemiologic, clinical and experimental reports indicate that nonsteroidal anti-inflammatory drugs (NSAIDs) could have a potential as anticancer agents. The aim of this study was the evaluation of cytotoxic potential in human glioblastoma cells of novel synthesized NSAID derivatives, obtained by linking, through a spacer, alpha-lipoic acid (ALA) to anti-inflammatory drugs, such as naproxen (AL-3, 11 and 17), flurbiprofen (AL-6, 13 and 19) and ibuprofen (AL-9, 15 and 21). The effects on the level of gene expression were also determined using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. According to our results, NSAID derivatives exhibited concentration dependent cytotoxic effects on U87-MG cell line when compared with the control group. Moreover, treatment of the most active compounds (AL-3, AL-6 and AL-9) caused upregulation of tumor suppressor gene PTEN and downregulation of some oncogenes such as AKT1, RAF1 and EGFR. In conclusion, our results revealed that AL-3, AL-6 and AL-9 could be suitable candidates for further investigation to develop new pharmacological strategies for the prevention of cancer.
Citation - WoS: 86
Citation - Scopus: 100
Antioxidative, Anticancer and Genotoxic Properties of Α-Pinene on N2a Neuroblastoma Cells
(Springer, 2013) Aydin, Elanur; Turkez, Hasan; Geyikoglu, Fatime
alpha-Pinene, an organic monoterpene, is found in essential oils of pine and coniferous trees. To date, although various biological activities of alpha-pinene have been demonstrated, its neurotoxicity has never been explored. Therefore in this study, we aimed to describe in vitro antiproliferative and/or cytotoxic properties by 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, genotoxic damage potentials by single cell gel electrophoresis, and oxidative effects by total antioxidant capacity (TAC) and total oxidative stress (TOS) analysis of alpha-pinene. Statistical analysis of MTT assay results indicated significant (p < 0.05) decreases of the cell proliferation rates in healthy neurons treated with alpha-pinene at only 400 mg/L, while significant decreases were observed in N2a cells at 100, 200 and 400 mg/L. On the other hand, the mean values of the total scores of cells showing DNA damage were not found significantly different from the control values on both cells. In addition, our results indicated that 10 and 25 mg/L of alpha-pinene treatment caused increases of TAC levels in primary rat neurons without any alterations of its level in N2a cells. However, alpha-pinene treatments at higher doses led to increases of TOS levels in both cell types. Overall our results suggest that alpha-pinene is of a limited therapeutic use as an anticancer agent.

Browse

Browsing * Akademik Arşiv by WoS Q "Q3"